US5230997A
Expired / Lapsed
US
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Methods of detecting the presence of human herpesvirus-7 infection
Assignee: United States Department of Health and Human Services
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Filing: 1990-07-19
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Granted: 1993-07-27
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Expires: 2010-07-19
Abstract
Abstract The present invention relates to a new human herpesvirus-7, proteins encoded in the genome of the virus, and antibodies specific for the virus and proteins. The virus was isolated from human peripheral blood mononuclear cells following incubation of the cells under conditions promoting T cell activation. Cultures of lymphocytes infected with the virus exhibited a cytopathic effect and electron microscopic analyses revealed a characteristic herpesvirus structure. The new virus is distinct from previously characterized human herpesviruses. The invention also relates to bioassays for the diagnosis of human herpesvirus-7 and the detection of human herpesvirus-7 in a biological sample. It further relates to a vaccine for humans against human herpesvirus-7.
Inventors
- 👤 Niza Frenkel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 20:12
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C07K16/00; C07K16/08; C07K16/081; C07K16/085; C07K16/087; C12; C12N; C12N7/00; C12Q; C12Q1/00; C12Q1/70; C12Q1/701; C12Q1/705; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; G01N33/56994; A; A61; A61K; A61K39/00; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16511; C12N2710/16522; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
80 words
A method for the diagnosis of human herpesvirus-7 infection in a human patient, comprising the steps of: ii) coating a substrate surface with a human herpesvirus-7 structural antigen ii) contacting said surface with serum suspected of containing human herpesvirus-7 specific antibodies from said human patient and iii) detecting the presence or absence of a complex formed between said antigen and antibodies specific thereof, if present in said serum, wherein the presence of said complex indicates the presence of said infection.
Claim 2
Independent Claim
80 words
A method for the diagnosis of human herpesvirus-7 infection in a human patient, comprising the steps of: ii) coating a substrate surface with a human herpesvirus-7 structural antigen ii) contacting said surface with serum suspected of containing human herpesvirus-7 specific antibodies from said human patient and iii) detecting the presence or absence of a complex formed between said antigen and antibodies specific thereof, if present in said serum, wherein the presence of said complex indicates the presence of said infection.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein said complex is detected by biotinylated antibodies.
Claim 4
Dependent Claim
13 words
The method of claim 2 wherein said biotinylated antibodies are detected by streptavidin.
Claim 5
Dependent Claim
16 words
The method of claim 1 wherein said complex is detected with fluorescein conjugated anti-human immunoglobulin G.
Claim 6
Dependent Claim
21 words
The method of claim 1 wherein said substrate surface is a gel, a slide, membrane, a microtitration plate, or column material.
Claim 7
Dependent Claim
16 words
The method of claim 1, wherein said antigen is displayed on the surface of human herpesvirus-7.
Claim 8
Independent Claim
79 words
A method for the diagnosis of human herpesvirus-7 infection in a human patient, comprising the steps of: i) coating a surface with antibodies a human herpesvirus-7 structural antigen; ii) obtaining a biological sample from said patient; iii) contacting said surface with said sample; and iv) detecting the presence or absence of a complex formed between said antibodies and virus, if present in said sample, wherein the presence of said complex indicates the presence of said infection in said patient.
Claim 9
Independent Claim
79 words
A method for the diagnosis of human herpesvirus-7 infection in a human patient, comprising the steps of: i) coating a surface with antibodies a human herpesvirus-7 structural antigen; ii) obtaining a biological sample from said patient; iii) contacting said surface with said sample; and iv) detecting the presence or absence of a complex formed between said antibodies and virus, if present in said sample, wherein the presence of said complex indicates the presence of said infection in said patient.
Claim 10
Dependent Claim
16 words
The method of claim 7, wherein said antigen is displayed on the surface of human herpesvirus-7.
Claim 11
Independent Claim
78 words
A method of detecting the presence of human herpesvirus-7 infection in a patient, comprising the steps of: obtaining a sample of serum suspected of containing herpesvirus-7 specific antibodies from said patient; contacting said sample of serum with a human herpesvirus-7 structural antigen; and determining if specific binding has occurred between said antigen and the antibodies, if contained in said serum sample, wherein the presence of said specific binding indicates the presence of human herpesvirus-7 infection in said patient.
Claim 12
Independent Claim
78 words
A method of detecting the presence of human herpesvirus-7 infection in a patient, comprising the steps of: obtaining a sample of serum suspected of containing herpesvirus-7 specific antibodies from said patient; contacting said sample of serum with a human herpesvirus-7 structural antigen; and determining if specific binding has occurred between said antigen and the antibodies, if contained in said serum sample, wherein the presence of said specific binding indicates the presence of human herpesvirus-7 infection in said patient.
Claim 13
Dependent Claim
26 words
The method of claim 9, wherein the determining step comprises a test selected from the group consisting of IFA, immunoblots, RIA, RIST, ELISA, agglutination and hemagglutination.
Claim 14
Dependent Claim
23 words
The method of claim 9, wherein said antigen comprises an immunogenic protein of human herpesvirus-7 having a molecular weight of approximately 83 kilodaltons.
Claim 15
Dependent Claim
20 words
The method of claim 9, wherein the contacting step comprises contacting said serum with proteins from human herpesvirus-7 infected cells.
Claim 16
Dependent Claim
20 words
The method of claim 12, additionally comprising electrophoresing said proteins from human herpesvirus-7 infected cells prior to the contacting step.
Claim 17
Dependent Claim
41 words
The method of claim 13, wherein the determining step comprises determining whether any antibodies in said serum component have specifically bound to protein having an apparent molecular weight of approximately 83 kilodaltons in the electrophoresis system used for the electrophoresis step.
Claim 18
Dependent Claim
35 words
The method of claim 13, wherein the electrophoresing step comprises subjecting said proteins to polyacrylamide gel electrophoresis, resulting in a gel with separated proteins, additionally comprising blotting the proteins in said gel to another material.
Claim 19
Dependent Claim
22 words
The method of claim 15, wherein the contacting step comprises a first contacting step wherein said serum is contacted with said material.
Claim 20
Dependent Claim
36 words
The method of claim 16, additionally comprising a second contacting step wherein antibodies specific for human herpesvirus-7 specific antibodies are contacted with said material, said second contacting step being performed subsequently to said first contacting step.
Claim 21
Dependent Claim
26 words
The method of claim 17, wherein the determining step comprises determining whether said antibodies specific for human herpesvirus-7 specific antibodies are specifically bound to said material.
Claim 22
Dependent Claim
17 words
The method of claim 17, wherein said antibodies specific for human herpesvirus-7 specific antibodies carry a label.
Claim 23
Dependent Claim
10 words
The method of claim 19, wherein said label comprises biotin.
Claim 24
Independent Claim
71 words
A method of detecting the presence of human herpesvirus-7 infection in a patient, comprising the steps of: obtaining a biological sample from said patient; contacting said biological sample with an antibody specific to a human herpesvirus-7 structural antigen; determining if specific binding has occurred between said antibody and said antigen present in said sample, wherein the presence of said specific binding indicates the presence of human herpesvirus-7 infection in said patient.
Claim 25
Independent Claim
71 words
A method of detecting the presence of human herpesvirus-7 infection in a patient, comprising the steps of: obtaining a biological sample from said patient; contacting said biological sample with an antibody specific to a human herpesvirus-7 structural antigen; determining if specific binding has occurred between said antibody and said antigen present in said sample, wherein the presence of said specific binding indicates the presence of human herpesvirus-7 infection in said patient.
Claim 26
Dependent Claim
16 words
A monoclonal antibody specific for a human herpesvirus-7 structural antigen, but not for other human herpesviruses.
Claim 27
Dependent Claim
16 words
A monoclonal antibody specific for a human herpesvirus-7 structural antigen, but not for other human herpesviruses.
Claim 28
Dependent Claim
18 words
An isolated antibody that specifically binds to a human herpesvirus-7 structural antigen, but not to any other herpesviruses.
Claim 29
Dependent Claim
18 words
An isolated antibody that specifically binds to a human herpesvirus-7 structural antigen, but not to any other herpesviruses.
Claim 30
Dependent Claim
20 words
An antibody according to claim 23, wherein said antibody does not specifically bind to any exposed epitope of human herpesvirus-6.
Claim 31
Dependent Claim
25 words
An antibody according to either claim 22 or claim 23, wherein said antibody is specific for an antigen displayed on the surface of human herpesvirus-7.
Claim 32
Dependent Claim
22 words
An antibody according to either claim 22 or claim 23, wherein said antigen is a protein encoded by human herpesvirus-7 DNA .
References Cited (Backward Citations)
No citation records indexed.
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