US5229282A
Expired / Lapsed
US
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Preparation of biosensor having a layer containing an enzyme, electron acceptor and hydrophilic polymer on an electrode system
Assignee: Panasonic Holdings Corp
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Filing: 1990-11-26
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Granted: 1993-07-20
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Expires: 2010-11-26
Abstract
Abstract The present invention relates to a process of preparation of a biosensor comprising forming an electrode system mainly containing carbon on an insulating base plate, treating the surface of electrode system with an organic solvent, and then arranging a reaction layer on the electrode system to give a unified element. The reaction layer contains an enzyme, electron acceptor and a hydrophilic polymer. Treatment with the organic solvent improves adhesion of the reaction layer to the electrode system. The electrode system contains a working electrode and a counter electrode. The electrode system is formed from a carbon paste containing a resin binder. Treatment is preferably carried out by wiping the surface of the electrodes with a material impregnated by the organic solvent.
Inventors
- 👤 Toshihiko Yoshioka
- 👤 Mariko Kawaguri
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 09:02
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
144 words
A process for preparation of a biosensor for determining electrochemically a change of substance concentration caused by reaction of an enzyme, an electron acceptor and a sample liquid, which consists essentially of arranging an electrode system made of carbon as a main component, wherein the carbon is provided by a carbon paste containing a resin binder and said electrode system consisting essentially of at least a working electrode and a counter electrode on an insulating base plate, contacting the surface of the working electrode and counter electrode with an organic solvent by wiping the surface of the electrodes with a material impregnated by an organic solvent, and arranging a reaction layer consisting of at least an enzyme, an electron acceptor and a hydrophilic polymer on the electrode system, said contacting with an organic solvent improving adhesion of the reaction layer to the electrode system.
Claim 2
Dependent Claim
144 words
A process for preparation of a biosensor for determining electrochemically a change of substance concentration caused by reaction of an enzyme, an electron acceptor and a sample liquid, which consists essentially of arranging an electrode system made of carbon as a main component, wherein the carbon is provided by a carbon paste containing a resin binder and said electrode system consisting essentially of at least a working electrode and a counter electrode on an insulating base plate, contacting the surface of the working electrode and counter electrode with an organic solvent by wiping the surface of the electrodes with a material impregnated by an organic solvent, and arranging a reaction layer consisting of at least an enzyme, an electron acceptor and a hydrophilic polymer on the electrode system, said contacting with an organic solvent improving adhesion of the reaction layer to the electrode system.
Claim 3
Dependent Claim
17 words
The process for preparation of a biosensor of claim 1, in which said enzyme is an oxidoreductase.
Claim 4
Dependent Claim
29 words
The process for preparation of a biosensor of claim 1, in which the electrode system consisting essentially of carbon is applied on an insulating base plate by screen printing.
Claim 5
Dependent Claim
32 words
The process for preparation of a biosensor of claim 1, in which the organic solvent is selected from the group consisting of methanol, ethanol, isopropyl alcohol, diethyl ether, n-hexane, heptane, and cyclohexane.
Claim 6
Dependent Claim
83 words
The process for preparation of a biosensor of claim 2, in which there is formed a first layer containing a hydrophilic polymer and an oxidoreductase on the electrodes contacted with an organic solvent, formed a second layer of a hydrophilic polymer by applying a solution of a hydrophilic polymer in an organic solvent on the first layer, and then formed a third layer containing an electron acceptor by applying a dispersion of the electron acceptor in an organic solvent on the second layer.
Claim 7
Dependent Claim
39 words
The process for preparation of a biosensor of claim 2, in which the reaction layer is formed by applying a solution comprising a hydrophilic polymer, an oxidoreductase, and an electron acceptor on the electrodes contacted with an organic solvent.
Claim 8
Dependent Claim
51 words
The process for preparation of a biosensor of claim 2, in which the reaction layer is formed by forming a layer containing a hydrophilic polymer on the electrodes contacted with an organic solvent, and applying a solution containing an oxidoreductase and an electron acceptor to said layer containing a hydrophilic polymer.
Claim 9
Dependent Claim
71 words
The process for preparation of a biosensor of claim 5, 6 or 7, in which the hydrophilic polymer of the reaction layer is selected from the group consisting of cellulose derivatives, gelatine or its derivatives, starch or its derivatives, homo or copolymer containing a residue derived from an unsaturated monomer selected from the group consisting of vinyl pyrrolidones, (meth)acrylic acid, salts thereof, maleic anhydride, salts thereof, (meth)acryl amide and salts thereof.
References Cited (Backward Citations)
US4418148A
Cited
US4897173A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-07-19 | 1997-01-18 | 1997-07-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-07-19 | 2001-01-18 | 2001-07-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-07-19 | 2005-01-18 | 2005-07-20 | $7700.00 | Window Closed / Lapsed |