US5227470A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for forming proteoliposome and method for forming giant proteoliposome
Assignee: Canon Inc
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Filing: 1992-08-19
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Granted: 1993-07-13
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Expires: 2012-08-19
Abstract
Abstract A method for forming a proteoliposome comprises incorporating into a liposome a membrane protein combined with a carrier. Further, a method for preparing a giant proteoliposome, comprises freezing and thawing an alkali metal salt solution containing a membrane protein and a lipid, and subsequently dialyzing against a second salt solution or a buffer solution having a lower osmotic pressure than that of said alkali metal salt solution. Further, a method for forming a proteoliposome, comprises freezing and thawing an alkali metal salt solution containing a membrane protein combined with a carrier and a lipid.
Inventors
- 👤 Tsunehiro Kanno
- 👤 Junji Ohyama
- 👤 Masanori Sakuranaga
- 👤 Hiroyoshi Kishi
- 👤 Nobuko Yamamoto
- 👤 Kinya Kato
- 👤 Harumi Iwashita
- 👤 Yasuko Tomida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 16:27
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1277; A61K9/1271; Y; Y10; Y10S; Y10S436/00; Y10S436/829; Y10S530/00; Y10S530/81; Y10S530/812
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
86 words
A method for forming a proteoliposome, comprising the steps of: (i) preparing a liposome; and (ii) selecting a carrier that will not enter a lipid layer of said liposome; (iii) attaching said carrier to a membrane protein; wherein said membrane protein is selected from a group consisting of metabolism-catalyzing enzymes, channel protein or peptides, pump protein or peptides, photoreceptive chromoproteins, neurotransmitter receptors, and hormone receptors; (iv) incorporating said membrane protein attached to said carrier into said liposome; and (v) disassociating said carrier from said membrane protein.
Claim 2
Independent Claim
86 words
A method for forming a proteoliposome, comprising the steps of: (i) preparing a liposome; and (ii) selecting a carrier that will not enter a lipid layer of said liposome; (iii) attaching said carrier to a membrane protein; wherein said membrane protein is selected from a group consisting of metabolism-catalyzing enzymes, channel protein or peptides, pump protein or peptides, photoreceptive chromoproteins, neurotransmitter receptors, and hormone receptors; (iv) incorporating said membrane protein attached to said carrier into said liposome; and (v) disassociating said carrier from said membrane protein.
Claim 3
Dependent Claim
22 words
The method of claim 1, wherein the volume of said carrier is equal to or more than the volume of said liposome.
Claim 4
Dependent Claim
28 words
The method of claim 1, wherein the shape of said carrier is spheric and the size thereof is equal to or larger than the diameter of said liposome.
Claim 5
Dependent Claim
32 words
The method of claim 1, wherein the shape of said carrier is planar or curved, and the radius of curvature thereof is equal to or larger than the radius of said liposome.
Claim 6
Dependent Claim
18 words
The method of claim 1, wherein said carrier is combined with hydrophilic side chains of said membrane protein.
Claim 7
Dependent Claim
13 words
The method of claim 1, wherein said membrane protein is a metabolism-catalyzing enzyme.
Claim 8
Dependent Claim
13 words
The method of claim 1, wherein said membrane protein is a photoreceptive chromoprotein.
Claim 9
Dependent Claim
13 words
The method of claim 1, wherein said membrane protein is a neurotransmitter receptor.
Claim 10
Dependent Claim
13 words
The method of claim 1, wherein said membrane protein is a hormone receptor.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1996-07-12 | 1997-01-11 | 1997-07-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-07-12 | 2001-01-11 | 2001-07-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-07-12 | 2005-01-11 | 2005-07-13 | $7700.00 | Window Closed / Lapsed |