US5215887A
Expired / Lapsed
US
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Glucose sensor measurement
Assignee: NEC Corp
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Filing: 1991-12-02
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Granted: 1993-06-01
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Expires: 2011-12-02
Abstract
Abstract Disclosed is a method of measuring glucose concentration of a sample solution using a glucose sensor having an ion-sensitive field-effect transistor which can detect pH change in the sample solution provided with a glucose oxidase-immobilized film formed on the ion-sensing section thereof, which comprise a step of measuring the output of the glucose sensor under a low level of dissolved oxygen in the enzyme-immobilized film; a step of measuring the output of the glucose sensor under a high level of dissolved oxygen in the enzyme-immobilized film; and a step of determining the glucose concentration of the sample solution from the difference between said two output values. The measuring system can be simplified and working efficiency can be improved since the present measuring method requires neither a buffer nor correct measuring of the sample solution or buffer.
Inventors
- 👤 Atsushi Saito
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 10:28
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; C12Q1/006
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
110 words
A method of measuring glucose concentration of a sample solution using a glucose sensor having an ion-sensitive field-effect transistor which can detect pH change in the sample solution and is provided with a glucose oxidase-immobilized film formed on an ion-sensing section thereof, which comprises: a step of measuring the output of the glucose sensor under a low level of dissolved oxygen in the glucose oxidase immobilized film; a step of measuring the output of the glucose sensor under a high level of dissolved oxygen in the glucose oxidase immobilized film; and a step of determining the glucose concentration of the sample solution from the difference between the two measured outputs.
Claim 2
Independent Claim
110 words
A method of measuring glucose concentration of a sample solution using a glucose sensor having an ion-sensitive field-effect transistor which can detect pH change in the sample solution and is provided with a glucose oxidase-immobilized film formed on an ion-sensing section thereof, which comprises: a step of measuring the output of the glucose sensor under a low level of dissolved oxygen in the glucose oxidase immobilized film; a step of measuring the output of the glucose sensor under a high level of dissolved oxygen in the glucose oxidase immobilized film; and a step of determining the glucose concentration of the sample solution from the difference between the two measured outputs.
Claim 3
Independent Claim
133 words
A method of measuring glucose concentration of a sample solution using a glucose sensor having an ion-sensitive field-effect transistor which can detect pH change in the sample solution and is provided with a glucose oxidase-immobilized film formed on an ion-sensing section thereof, which comprises; a step of measuring the output of the glucose sensor under a low level of dissolved oxygen in the glucose oxidase immobilized film by exposing the sample solution to a gaseous atmosphere having an oxygen content lower than in air; a step of measuring the output of the glucose sensor under a high level of dissolved oxygen in the glucose enzyme-immobilized film by exposing the sample solution to air; and a step of determining the glucose concentration of the sample solution from the difference between the two measured outputs.
Claim 4
Independent Claim
133 words
A method of measuring glucose concentration of a sample solution using a glucose sensor having an ion-sensitive field-effect transistor which can detect pH change in the sample solution and is provided with a glucose oxidase-immobilized film formed on an ion-sensing section thereof, which comprises; a step of measuring the output of the glucose sensor under a low level of dissolved oxygen in the glucose oxidase immobilized film by exposing the sample solution to a gaseous atmosphere having an oxygen content lower than in air; a step of measuring the output of the glucose sensor under a high level of dissolved oxygen in the glucose enzyme-immobilized film by exposing the sample solution to air; and a step of determining the glucose concentration of the sample solution from the difference between the two measured outputs.
Claim 5
Independent Claim
134 words
A method of measuring glucose concentration of a sample solution using a glucose sensor having an ion-sensitive field-effect transistor which can detect pH change in the sample solution and is provided with a glucose oxidase-immobilized film formed on an ion-sensing section thereof, which comprises: a step of measuring the output of the glucose sensor under a low level of dissolved oxygen in the glucose oxidase immobilized film by exposing the sample solution to air; a step of measuring the output of the glucose sensor under a high level of dissolved oxygen in the glucose oxidase immobilized film by exposing the sample solution to a gaseous atmosphere having an oxygen content higher than in air; and a step of determining the glucose concentration of the sample solution from the difference between the two measured outputs.
Claim 6
Independent Claim
134 words
A method of measuring glucose concentration of a sample solution using a glucose sensor having an ion-sensitive field-effect transistor which can detect pH change in the sample solution and is provided with a glucose oxidase-immobilized film formed on an ion-sensing section thereof, which comprises: a step of measuring the output of the glucose sensor under a low level of dissolved oxygen in the glucose oxidase immobilized film by exposing the sample solution to air; a step of measuring the output of the glucose sensor under a high level of dissolved oxygen in the glucose oxidase immobilized film by exposing the sample solution to a gaseous atmosphere having an oxygen content higher than in air; and a step of determining the glucose concentration of the sample solution from the difference between the two measured outputs.
Claim 7
Independent Claim
129 words
A method of measuring glucose concentration of a sample solution using a glucose sensor having an ion-sensitive field-effect transistor which can detect pH change in the sample solution and is provided with a glucose oxidase-immobilized film formed on an ion-sensing section thereof, which comprises: a step of measuring the output of the glucose sensor by dipping the glucose sensor in the sample solution under a low level of dissolved oxygen in the glucose oxidase immobilized film; a step of measuring the output of the glucose under a high level of dissolved oxygen in the glucose oxidase immobilized film by exposing the glucose sensor to a gaseous atmosphere containing oxygen; and a step of determining the glucose concentration of the sample solution from the difference between the two measured outputs.
Claim 8
Independent Claim
129 words
A method of measuring glucose concentration of a sample solution using a glucose sensor having an ion-sensitive field-effect transistor which can detect pH change in the sample solution and is provided with a glucose oxidase-immobilized film formed on an ion-sensing section thereof, which comprises: a step of measuring the output of the glucose sensor by dipping the glucose sensor in the sample solution under a low level of dissolved oxygen in the glucose oxidase immobilized film; a step of measuring the output of the glucose under a high level of dissolved oxygen in the glucose oxidase immobilized film by exposing the glucose sensor to a gaseous atmosphere containing oxygen; and a step of determining the glucose concentration of the sample solution from the difference between the two measured outputs.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-05-31 | 1996-11-30 | 1997-06-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-05-31 | 2000-11-30 | 2001-06-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-05-31 | 2004-11-30 | 2005-06-01 | $7700.00 | Window Closed / Lapsed |