Fluorescence measurement apparatus with an automatic recorder full-scale setting function

Claim 2

A fluorescence measurement apparatus for analyzing a spectrum curve of fluorescent light emitted from a sample irradiated by an excitation light, comprising: an excitation light generator; an emitted light detector; scanning means for holding one of the emitted light detector and the excitation light generator at a fixed wavelength and scanning the other of the emitted light detector and excitation light generator over a range of wavelengths; a memory for storing data of the spectrum curve; and analyzing means for analyzing the stored data to determine data corresponding to peaks of the spectrum curve about multiples of the fixed wavelength and data corresponding to minima of the spectrum curve adjacent the determined peaks, and for generating a corrected spectrum curve by disregarding data between the determined minima corresponding to the determined peaks; scale setting means for detecting a maximum peak value of the corrected spectrum curve and setting a scale based on the detected maximum peak value; and a recorder for recording the corrected spectrum curve, wherein a full scale of the recorder is set to the scale set by the scale setting means.

Claim 3

The fluorescence measurement apparatus of claim 1, wherein the analyzing means further determines data corresponding to a Raman effect peak of the spectrum curve and minima adjacent the Raman effect peak, and further generates the corrected spectrum curve by removing from the memory data between the determined minima corresponding to the Raman effect peak.

Claim 4

A fluorescence measurement apparatus for analyzing a spectrum curve of fluorescent light emitted from a sample irradiated by an excitation light, comprising: an excitation light generator; an emitted light detector; scanning means for holding one of the emitted light detector and the excitation light generator at a fixed wavelength and scanning the other of the emitted light detector and excitation light generator over a range of wavelengths; a memory for storing data of the spectrum curve; and analyzing means for analyzing the stored data to determine data corresponding to peaks of the spectrum curve about multiples of the fixed wavelength and data corresponding to minima of the spectrum curve adjacent the determined peaks, and for generating a corrected spectrum curve by removing from the memory data between the determined minima corresponding to the determined peaks; scale setting means for detecting a maximum peak value of the corrected spectrum curve and setting a scale based on the detected maximum peak value; and a recorder for recording the corrected spectrum curve, wherein a full scale of the recorder is set to the scale set by the scale setting means.

Claim 5

A fluorescence measurement apparatus for analyzing a spectrum curve of fluorescent light emitted from a sample irradiated by an excitation light, comprising: an excitation light generator; an emitted light detector; scanning means for holding one of the emitted light detector and the excitation light generator at a fixed wavelength and scanning the other of the emitted light detector and excitation light generator over a range of wavelengths; a memory for storing data of the spectrum curve; and analyzing means for analyzing the stored data to determine data corresponding to peaks of the spectrum curve about multiples of the fixed wavelength and data corresponding to minima of the spectrum curve adjacent the determined peaks, and for generating a corrected spectrum curve by removing from the memory data between the determined minima corresponding to the determined peaks; scale setting means for detecting a maximum peak value of the corrected spectrum curve and setting a scale based on the detected maximum peak value; and a recorder for recording the corrected spectrum curve, wherein a full scale of the recorder is set to the scale set by the scale setting means.

Claim 6

The fluorescence measurement apparatus of claim 3, wherein the analyzing means removes data from the memory corresponding to a Rayleigh scattered light peak having a wavelength substantially equal to the fixed wavelength.

Claim 7

The fluorescence measurement apparatus of claim 4, wherein the data corresponding to peaks of the spectrum curve about integer multiples of the fixed wavelength are generated by diffractions of Rayleigh scattered light.

Claim 8

The fluorescence measurement apparatus of claim 3, wherein the scanning means is in an excitation spectrum measurement mode such that the emitted light detector is held at the fixed wavelength and the excitation light generator is varied.

Claim 9

The fluorescence measurement apparatus of claim 6, wherein the analyzing means analyzes the stored data to determine peaks corresponding to integer multiples of the fixed wavelength.

Claim 10

The fluorescence measurement apparatus of claim 3, wherein the scanning means is in an emission spectrum measurement mode such that the excitation light generator is held at the fixed wavelength and the emitted light detector is varied.

Claim 11

The fluorescence measurement apparatus of claim 8, wherein the analyzing means analyzes the stored data to determine peaks corresponding to integer submultiples of the fixed wavelength.

Claim 12

The fluorescence measurement apparatus of claim 3, wherein the analyzing means further determines data corresponding to a Raman effect peak of the spectrum curve and minima adjacent the Raman effect peak, and further generates the corrected spectrum curve by removing from the memory data between the determined minima corresponding to the Raman effect peak.

Claim 13

A fluorescence measurement apparatus for analyzing a spectrum curve of fluorescent light emitted from a sample irradiated by an excitation light, comprising: an excitation light generator; an emitted light detector; scanning means for holding one of the emitted light detector and the excitation light generator at a fixed wavelength and scanning the other of the emitted light detector and excitation light generator over a range of wavelengths; a memory for storing data of the spectrum curve; and analyzing means for analyzing the stored data to determine data corresponding to peaks of the spectrum curve about multiples of the fixed wavelength and data corresponding to minima of the spectrum curve adjacent the determined peaks, and for generating a corrected spectrum curve by marking the data between the determined minima corresponding to the determined peaks; scale setting means for detecting a maximum peak value of unmarked data of the corrected spectrum curve and setting a scale based on the detected maximum peak value; and a recorder for recording the corrected spectrum curve, wherein a full scale of the recorder is set to the scale set by the scale setting means.

Claim 14

A fluorescence measurement apparatus for analyzing a spectrum curve of fluorescent light emitted from a sample irradiated by an excitation light, comprising: an excitation light generator; an emitted light detector; scanning means for holding one of the emitted light detector and the excitation light generator at a fixed wavelength and scanning the other of the emitted light detector and excitation light generator over a range of wavelengths; a memory for storing data of the spectrum curve; and analyzing means for analyzing the stored data to determine data corresponding to peaks of the spectrum curve about multiples of the fixed wavelength and data corresponding to minima of the spectrum curve adjacent the determined peaks, and for generating a corrected spectrum curve by marking the data between the determined minima corresponding to the determined peaks; scale setting means for detecting a maximum peak value of unmarked data of the corrected spectrum curve and setting a scale based on the detected maximum peak value; and a recorder for recording the corrected spectrum curve, wherein a full scale of the recorder is set to the scale set by the scale setting means.

Claim 15

The fluorescence measurement apparatus of claim 11, wherein the analyzing means removes data from the memory corresponding to a Rayleigh scattered light peak having a wavelength substantially equal to the fixed wavelength.

Claim 16

The fluorescence measurement apparatus of claim 12, wherein the data corresponding to peaks of the spectrum curve about integer multiples of the fixed wavelength are generated by diffractions of Rayleigh scattered light.

Claim 17

The fluorescence measurement apparatus of claim 11, wherein the scanning means is in an excitation spectrum measurement mode such that the emitted light detector is held at the fixed wavelength and the excitation light generator is varied.

Claim 18

The fluorescence measurement apparatus of claim 14, wherein the analyzing means analyzes the stored data to determine peaks corresponding to integer multiples of the fixed wavelength.

Claim 19

The fluorescence measurement apparatus of claim 11, wherein the scanning means is in an emission spectrum measurement mode such that the excitation light generator is held at the fixed wavelength and the emitted light detector is varied.

Claim 20

The fluorescence measurement apparatus of claim 16, wherein the analyzing means analyzes the stored data to determine peaks corresponding to integer submultiples of the fixed wavelength.

Claim 21

The fluorescence measurement apparatus of claim 11, wherein the analyzing means further determines data corresponding to a Raman effect peak of the spectrum curve and minima adjacent the Raman effect peak, and further generates the corrected spectrum curve by marking data between the determined minima corresponding to the Raman effect peak.