Automatic air temperature cycler and method of use in polymerose chain reaction

Claim 1

In a device employing a polymerase for replicating a particular sequence of DNA or RNA and for amplifying the concentration thereof in vessels containing samples to be treated, said device includes a chamber means having means for developing and maintaining a selectable, predetermined temperature profile in said chamber means comprising a heater means, temperature cycling means, and temperature control means, the improvement comprising means for repetitively and uninterruptedly establishing and effectively controlling each of successive temperatures of said profile for a selectable time duration in a predetermined sequence of temperatures in said selectable, predetermined temperature profile, said improvement including sample treatment means comprising circulating means for distributing a heat transfer gas from ambient atmosphere in a continuous, efficient heat-exchanging mode to envelope vessels containing samples of a DNA fraction in said chamber means thereby, treating the samples thermally in sequential steps for regulated time periods defining a selectable, cyclical incubation-promoting protocol, first temperature sensor means in said chamber means for monitoring the temperature of air moving through said chamber means, second temperature sensor means including probe means projecting into one of said vessels, for sensing the temperature of the liquid samples contained in vessels containing samples to be treated, first control means functionally coupling said first temperature sensor means to said heater means for regulating said heater means to maintain a selectable program in said chamber means, consistent with said selectable, predetermined temperature profile, second control means functionally coupling said second temperature sensor means to said heater means for regulating said heater means to maintain a selectable predetermined temperature profile in said samples in said vessels corresponding to said protocol, rack means for supporting vessel-housed samples, and hanger means for supporting said rack means in said chamber means, said rack means being of a low gas flow interfering configuration to preclude impedance of and interference with circulation of heat transfer gas about the vessel-housed samples.