US5175103A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Preparation of pure cultures of post-mitotic human neurons
Assignee: TRUSTEES OF UNIVERSITY OF PENNSYLVANIA A NON-PROFIT CORP OF PENNSYLVANIA
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Filing: 1991-10-21
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Granted: 1992-12-29
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Expires: 2011-10-21
Abstract
Abstract NTera 2/cl.D1 (NT2) cells, a human teratocarcinoma cell line, were manipulated following retinoic acid (RA) treatment to yield>95% pure cultures of neuronal cells (NT2-N cells). This culture method is capable of yielding sufficient highly differentiated post-mitotic NT2-N cells for both biochemical and molecular biological studies. NT2 cells can be transfected efficiently and the transfected gene products can be expressed in both NT2 and NT2-N cells.
Inventors
- 👤 Virginia Lee
- 👤 Samuel Pleasure
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 01:58
CPC Classifications
B; B29; B29C; B29C41/00; B29C41/02; B29C41/12; B29C41/34; B29C41/36; C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0618; C12N5/0619; B29L; B29L2023/00; C12N2501/00; C12N2501/30; C12N2501/38; C12N2501/385; C12N2506/00; C12N2506/02; C12N2506/30
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
60 words
A method for producing a stable at least 95% pure population of post-mitotic human neurons expressing exogenous gene products comprising: transfecting at least one plasmid including a selectable marker into cultured NTera2/D1 cells; culturing said NTera2/D1 cells with retinoic acid to obtain a multi-layer culture; dispersing said cultured cells; and culturing said dispersed cells with cytosine arabinoside, fluorodeoxyuridine and uridine.
Claim 2
Independent Claim
60 words
A method for producing a stable at least 95% pure population of post-mitotic human neurons expressing exogenous gene products comprising: transfecting at least one plasmid including a selectable marker into cultured NTera2/D1 cells; culturing said NTera2/D1 cells with retinoic acid to obtain a multi-layer culture; dispersing said cultured cells; and culturing said dispersed cells with cytosine arabinoside, fluorodeoxyuridine and uridine.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein said plasmid comprises a β-galactosidase expression plasmid.
Claim 4
Independent Claim
40 words
A method for preparing an at least 95% pure culture of post-mitotic human neurons comprising: culturing NTera2/D1 cells with retinoic acid to obtain a multi-layered culture; dispersing said cultured cells; culturing said dispersed cells with cytosine arabinoside, fluorodeoxyuridine and uridine.
Claim 5
Independent Claim
40 words
A method for preparing an at least 95% pure culture of post-mitotic human neurons comprising: culturing NTera2/D1 cells with retinoic acid to obtain a multi-layered culture; dispersing said cultured cells; culturing said dispersed cells with cytosine arabinoside, fluorodeoxyuridine and uridine.
References Cited (Backward Citations)
US4829000A
Cited
US5032407A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-12-29 | 1996-06-29 | 1996-12-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-12-29 | 2000-06-29 | 2000-12-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-12-29 | 2004-06-29 | 2004-12-29 | $7700.00 | Window Closed / Lapsed |