US5173407A
Expired / Lapsed
US
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Method for measuring glycosyltransferase
Assignee: Konica Minolta Inc
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Filing: 1991-01-22
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Granted: 1992-12-22
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Expires: 2011-01-22
Abstract
Abstract There is disclosed a method for measuring a glycosyltransferase, for measuring an activity or a concentration of the glycosyltransferase for a specific sugar, which comprises using a constitution containing a donor which is not labelled and a substance which is specifically bound only to a product.
Inventors
- 👤 Morito Uemura
- 👤 Shin-ya Yoshida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 06:51
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/581; C; C12; C12Q; C12Q1/00; C12Q1/48; Y; Y10; Y10S; Y10S436/00; Y10S436/827; Y10T; Y10T436/00; Y10T436/15; Y10T436/153333
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
149 words
A method for directly measuring a concentration of a galactosyltransferase by measuring an activity of said galactosyltransferase in a serum, comprising: adding a non-labeled uridine diphosphate-sugar having a first sugar, a non-labeled substance, a labeled acceptor having a second sugar and a non-labeled acceptor to which said first sugar is to be transferred by said galactosyltransferase in said serum, wherein said non-labeled acceptor is selected from the group consisting of monosaccharides, oligosaccharides, glycoproteins and glycolipids, said non-labeled substance is a lectin or an antibody and is specifically bound only to the non-labeled acceptor to which the sugar has been transferred, and said labeled acceptor comprises a radioisotope, an enzyme or a fluorescent substance, said non-labeled substance is immobilized to a solid phase; incubating said serum to effect enzymatic reaction, washing said solid phase; and measuring said non-labeled acceptor to which the sugar has been transferred on said solid phase.
Claim 2
Independent Claim
149 words
A method for directly measuring a concentration of a galactosyltransferase by measuring an activity of said galactosyltransferase in a serum, comprising: adding a non-labeled uridine diphosphate-sugar having a first sugar, a non-labeled substance, a labeled acceptor having a second sugar and a non-labeled acceptor to which said first sugar is to be transferred by said galactosyltransferase in said serum, wherein said non-labeled acceptor is selected from the group consisting of monosaccharides, oligosaccharides, glycoproteins and glycolipids, said non-labeled substance is a lectin or an antibody and is specifically bound only to the non-labeled acceptor to which the sugar has been transferred, and said labeled acceptor comprises a radioisotope, an enzyme or a fluorescent substance, said non-labeled substance is immobilized to a solid phase; incubating said serum to effect enzymatic reaction, washing said solid phase; and measuring said non-labeled acceptor to which the sugar has been transferred on said solid phase.
Claim 3
Dependent Claim
19 words
The method for measuring a glycosyltransferase according to claim 1, wherein said labeled acceptor is labeled by a radioisotope.
Claim 4
Dependent Claim
16 words
The method for measuring a glycosyltransferase according to claim 2, wherein said radioisotope is 125 I.
Claim 5
Dependent Claim
16 words
The method for measuring a glycosyltransferase according to claim 2, wherein said radioisotope is 3 H.
Claim 6
Dependent Claim
23 words
The method for measuring a glycosyltransferase according to claim 1, wherein said labeled acceptor is labeled by an enzyme or a fluorescent substance.
Claim 7
Dependent Claim
16 words
The method for measuring a galactosyltransferase according to claim 1, wherein said radioisotope is 125 I.
Claim 8
Dependent Claim
35 words
The method for measuring a galactosyltransferase according to claim 1, wherein said non-labeled substance which is capable of binding specifically to the non-labeled acceptor to which the sugar has been transferred is a monoclonal antibody.
Claim 9
Dependent Claim
49 words
The method for measuring a galactosyltransferase according to claim 1, wherein said substance which is capable of binding specifically to the non-labeled acceptor to which the sugar has been transferred is RCA or a monoclonal antibody which is specifically bound only to a sugar chain containing non-reducing terminal galactose.
Claim 10
Dependent Claim
40 words
The method for measuring a galactosyltransferase according to claim 1, wherein said non-labeled substance which is capable of binding specifically to the non-labeled acceptor to which the sugar has been transferred is directly or indirectly adsorbed to said solid phase.
Claim 11
Dependent Claim
60 words
The method for measuring a galactosyltransferase according to claim 1, wherein said non-labeled substance which is capable of binding specifically to the non-labeled acceptor to which the sugar has been transferred is RCA or a monoclonal antibody which is specifically bound only to a sugar chain containing non-reducing terminal galactose, and is directly or indirectly adsorbed to said solid phase.
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