US5149796A
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Chimeric DNA-RNA catalytic sequences
Assignee: City of Hope
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Filing: 1991-04-30
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Granted: 1992-09-22
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Expires: 2011-04-30
Abstract
Abstract This invention provides chimeric DNA/RNA catalytic molecules useful to cleave RNA sequences.
Inventors
- 👤 John J. Rossi
- 👤 Pairoj Chang
- 👤 Bruce E. Kaplan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 12:59
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1131; C12N15/1132; A; A61; A61K; A61K38/00; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111; C12N2310/12; C12N2310/121; C12N2310/30; C12N2310/32; C12N2310/322
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
100 words
A catalytic molecule capable of cleaving an RNA sequence at a known ribozyme cleavage site said molecule having the formula 3' X - AAAG - Y - AGUAAGUC - Z 5' or 3' X - CAAAG - Y - AGUAAGUC - Z 5' in which X and Z are DNA sequences that base pair with an RNA substrate at positions juxtaposed to said known cleavage site, AAAG, CAAAG and AGUAGUC are RNA sequences, Y is a DNA sequence that base pairs inter se in a manner required to permit said RNA sequences to cleave said substrate at said cleavage site.
Claim 2
Dependent Claim
100 words
A catalytic molecule capable of cleaving an RNA sequence at a known ribozyme cleavage site said molecule having the formula 3' X - AAAG - Y - AGUAAGUC - Z 5' or 3' X - CAAAG - Y - AGUAAGUC - Z 5' in which X and Z are DNA sequences that base pair with an RNA substrate at positions juxtaposed to said known cleavage site, AAAG, CAAAG and AGUAGUC are RNA sequences, Y is a DNA sequence that base pairs inter se in a manner required to permit said RNA sequences to cleave said substrate at said cleavage site.
Claim 3
Dependent Claim
50 words
A catalytic molecule capable of cleaving an RNA sequence, said molecule having catalytic RNA moieties linked to first and second DNA moieties which base pair with the substrate RNA sequences flanking the cleavage site and interconnected by a third DNA sequence which base pairs inter se to facilitate said cleavage.
Claim 4
Dependent Claim
50 words
A catalytic molecule capable of cleaving an RNA sequence, said molecule having catalytic RNA moieties linked to first and second DNA moieties which base pair with the substrate RNA sequences flanking the cleavage site and interconnected by a third DNA sequence which base pairs inter se to facilitate said cleavage.
Claim 5
Dependent Claim
12 words
A molecule including the construct shown by FIG. 1 or FIG. 2.
Claim 6
Dependent Claim
12 words
A molecule including the construct shown by FIG. 1 or FIG. 2.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-09-22 | 1996-03-23 | 1996-09-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-22 | 2000-03-23 | 2000-09-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-22 | 2004-03-23 | 2004-09-22 | $7700.00 | Window Closed / Lapsed |