US5149780A
Expired / Lapsed
US
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Peptides and antibodies that inhibit integrin-ligand binding
Assignee: Scripps Research Institute
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Filing: 1988-10-03
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Granted: 1992-09-22
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Expires: 2008-10-03
Abstract
Abstract Polypeptides which are derived from the Arg-Gly-Asp (RGD) binding portion of an Integrin beta subunit are disclosed as are their use for modulation of Integrin ligand binding. Anti-antibody peptides, hybridomas secreting these antibodies, as well as methods of making any using such antibodies, and recombinant DNA molecules that define the structural gene coding for the polypeptides are also contemplated within the scope of the present invention.
Inventors
- 👤 Edward F. Plow
- 👤 Stanley E. D'Souza
- 👤 Mark H. Ginsberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:23
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/2839; C07K16/2848; C07K14/00; C07K14/435; C07K14/705; C07K14/70546; C07K14/70557; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/566; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
27 words
A polypeptide of 60 to 90 amino acid residues in length having a sequence homologous to the GPIIIa amino acid residue sequence represented by the formula: DYPVDIYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAFVDKPVSPYMYISPPE.
Claim 2
Dependent Claim
27 words
A polypeptide of 60 to 90 amino acid residues in length having a sequence homologous to the GPIIIa amino acid residue sequence represented by the formula: DYPVDIYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAFVDKPVSPYMYISPPE.
Claim 3
Dependent Claim
18 words
A polypeptide corresponding in amino acid residue sequence to the formula: a) DYPVIDYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAFVDKPVSPYMYISPPE, b) GYPIDLYYLMDLSYSMLDDLRNVKKLGGDLLRALNEITESGRIGFGSFVDKTVLPFVNTHP, or c) DYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTP.
Claim 4
Dependent Claim
18 words
A polypeptide corresponding in amino acid residue sequence to the formula: a) DYPVIDYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAFVDKPVSPYMYISPPE, b) GYPIDLYYLMDLSYSMLDDLRNVKKLGGDLLRALNEITESGRIGFGSFVDKTVLPFVNTHP, or c) DYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTP.
Claim 5
Dependent Claim
37 words
A recombinant DNA molecule comprising a vector operatively linked to a DNA segment that defines a structural gene coding for a polypeptide according to claim 1, wherein said structural gene contains no more than 2,000 base pairs.
Claim 6
Dependent Claim
19 words
The recominant DNA molecule of claim 5 wherein said structural gene codes for a polypeptide according to claim 2.
Claim 7
Dependent Claim
21 words
The recombinant DNA molecule of claim 3 wherein said vector is capable of expressing said structural gene in a host cell.
Claim 8
Dependent Claim
23 words
The recombinant DNA molecule of claim 3 wherein said structural gene has a nucleotide base sequence represented by the formula: GATTACCCTGTGGACATCTACTACTTGATGGACCTGTCTTACTCCATGAAGGATGATCTGTGGA GCATCCAGAACCTGGGTACCAAGCTGGCCACCCAGATGCGAAAGCTCACCAGTAACCTGCGGATT GGCTTCGGGGCATTTGTGGACAAGCCTGTGTCACCATACATGTATATCTCCCCACCAGAG.
Claim 9
Dependent Claim
59 words
A nucleotide segment containing no more than 12,000 nucleotide base pairs including a sequence defining a structural gene coding for a polypeptide of 60 to 90 amino acid residues in length having a sequence homologous to the GPIIIa amino acid residue sequence represented by the formula: DYPVDIYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAGVDKPVSPYMYISPPE, wherein said structural gene sequence contains no more than 2,000 base pairs.
Claim 10
Dependent Claim
59 words
A nucleotide segment containing no more than 12,000 nucleotide base pairs including a sequence defining a structural gene coding for a polypeptide of 60 to 90 amino acid residues in length having a sequence homologous to the GPIIIa amino acid residue sequence represented by the formula: DYPVDIYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAGVDKPVSPYMYISPPE, wherein said structural gene sequence contains no more than 2,000 base pairs.
Claim 11
Dependent Claim
22 words
The nucleotide segment of claim 7 wherein said structural gene has a nucleotide base sequence represented by the formula: GATTACCCTGTGGACATCTACTACTTGATGGACCTGTCTTACTCCATGAAGGATGATCTGTGGA GCATCCAGAACCTGGGTACCAAGCTGGCCACCCAGATGCGAAAGCTCACCAGTAACCTGCGGATT GGCTTCGGGGCATTTGTGGACAAGCCTGTGTCACCATACATGTATATCTCCCCACCAGAG.
Claim 12
Dependent Claim
35 words
A monoclonal antibody comprising antibody molecules that immunoreact with a) a polypeptide according to claim 1, and b) the beta subunit of an Integrin to which the amino acid residue sequence of said polypeptide corresponds.
Claim 13
Dependent Claim
20 words
A monoclonal antibody comprising antibody molecules that immunoreact with a) GPIIIa, and b) a polypeptide corresponding to the formula: DYPVDIYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAFVDKPUSPYMYISPPE.
Claim 14
Dependent Claim
20 words
A monoclonal antibody comprising antibody molecules that immunoreact with a) GPIIIa, and b) a polypeptide corresponding to the formula: DYPVDIYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAFVDKPUSPYMYISPPE.
Claim 15
Dependent Claim
26 words
A monoclonal antibody comprising antibody molecules that immunoreact with a) the beta subunit of the LeuCam Integran and b) a polypeptide corresponding to the formula: GYPIDLYYLMDLSYSMLDDLRNVKKLGGDLLRALNEITESGRIGFGSFVDKTVLPFVNTHP.
Claim 16
Dependent Claim
26 words
A monoclonal antibody comprising antibody molecules that immunoreact with a) the beta subunit of the LeuCam Integran and b) a polypeptide corresponding to the formula: GYPIDLYYLMDLSYSMLDDLRNVKKLGGDLLRALNEITESGRIGFGSFVDKTVLPFVNTHP.
Claim 17
Dependent Claim
26 words
A monoclonal antibody comprising antibody molecules that immunoreact with a) the beta subunit of the VLA Integran, and b) a polypeptide corresponding to the formula: DYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDRFIGFGSFVEKTVMPYISTTP.
Claim 18
Dependent Claim
26 words
A monoclonal antibody comprising antibody molecules that immunoreact with a) the beta subunit of the VLA Integran, and b) a polypeptide corresponding to the formula: DYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDRFIGFGSFVEKTVMPYISTTP.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-09-22 | 1996-03-23 | 1996-09-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-22 | 2000-03-23 | 2000-09-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-22 | 2004-03-23 | 2004-09-22 | $7700.00 | Window Closed / Lapsed |