US5144019A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Ribozyme cleavage of HIV-I RNA
Assignee: City of Hope
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Filing: 1989-06-21
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Granted: 1992-09-01
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Expires: 2009-06-21
Abstract
Abstract Stable, catalytic efficient ribozymes useful, inter alia, to cleave HIV-I RNA or any other viral or endogenous cellular RNA, in vitro and in vivo, cells transformed with such ribozymes and the use of such ribozymes and cells for anti-AIDs therapy are disclosed.
Inventors
- 👤 John J. Rossi
- 👤 Edouard M. Cantin
- 👤 John A. Zaia
- 👤 Pairoj Chang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 03:41
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1131; C12N15/1132; A; A61; A61K; A61K38/00; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111; C12N2310/12; C12N2310/121
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
79 words
A snythetic catalytic RNA ribozyme strand targeted to a region of HIV-I RNA having the sequence GAAAC(X) n GU in which X is any nucleotide and the value of n is from 0 to about 50, said snythetic catalytic ribozyme strand having the formula 3' YAGNAGUCZ 5' in which N is any nucleotide, Y and Z are flanking sequences of at least five nucleotides complementary to sequences adjacent to cleavage site in the GAAAC(X) n GU target RNA sequence.
Claim 2
Dependent Claim
79 words
A snythetic catalytic RNA ribozyme strand targeted to a region of HIV-I RNA having the sequence GAAAC(X) n GU in which X is any nucleotide and the value of n is from 0 to about 50, said snythetic catalytic ribozyme strand having the formula 3' YAGNAGUCZ 5' in which N is any nucleotide, Y and Z are flanking sequences of at least five nucleotides complementary to sequences adjacent to cleavage site in the GAAAC(X) n GU target RNA sequence.
Claim 3
Dependent Claim
65 words
A ribozyme comprising an RNA hybrid having a target strand and catalytic strand said target strand having including the sequence GAAAC(X) n GU said catalytic strand inclluding the sequence 3' YAGNAGUCZ 5' in which N is any nucleotide and in which flanking sequences Y and Z are each sequences of at least five nucleotides complementary to sequences adjacent a dleavage site in said target strand.
Claim 4
Dependent Claim
65 words
A ribozyme comprising an RNA hybrid having a target strand and catalytic strand said target strand having including the sequence GAAAC(X) n GU said catalytic strand inclluding the sequence 3' YAGNAGUCZ 5' in which N is any nucleotide and in which flanking sequences Y and Z are each sequences of at least five nucleotides complementary to sequences adjacent a dleavage site in said target strand.
Claim 5
Dependent Claim
7 words
A ribozyme construct having the formula: ##STR12##
Claim 6
Dependent Claim
7 words
A ribozyme construct having the formula: ##STR12##
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-09-01 | 1996-03-02 | 1996-09-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-01 | 2000-03-02 | 2000-09-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-01 | 2004-03-02 | 2004-09-01 | $7700.00 | Window Closed / Lapsed |