US5138170A
Expired / Lapsed
US
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Method and apparatus for identifying chromosomes or cells
Assignee: National Institute of Advanced Industrial Science and Technology
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Filing: 1991-05-20
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Granted: 1992-08-11
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Expires: 2011-05-20
Abstract
Abstract A method and an apparatus for sorting chromosomes or cells by utilizing a difference in fluorescent profilepatterns. A linear laser beam having a high beam intensity is formed from several fringe patterns, and fluorescence intensities from local portions of chromosome or cell are measured in real-time. Resolution of chromosome or cell is improved. A type of flowing chromosome or cell which permits the rotational conditions within a range can be sorted accurately and rapidly.
Inventors
- 👤 Yoshio Noguchi
- 👤 Yoshinobu Uchibori
- 👤 Mikio Yamashita
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 16:29
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1456; G01N15/1459; G01N15/1434; G01N15/1436; G01N15/149; G01N2015/1447; G01N2015/145; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N2021/6439; G01N2021/6441
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
114 words
A method for identifying a chromosome or cell comprising the steps of: irradiating a flow-stream containing chromosomes or cells which have been previously labelled with a fluorescent material with a laser beam of only one interference fringe among interference laser beams having a fringe pattern perpendicular to a flow axis along which said chromosomes or cells flow; measuring fluorescence intensity emitted from the fluorescent material on said chromosomes or cells; comparing a fluorescent profile-pattern obtained from variation of said fluorescent intensity with time with preset standard fluorescent profile-patterns of chromosome or cell; and sorting said chromosomes or cells on the basis of the comparison of said fluorescent profile-pattern with said preset standard fluorescent profile-patterns.
Claim 2
Independent Claim
114 words
A method for identifying a chromosome or cell comprising the steps of: irradiating a flow-stream containing chromosomes or cells which have been previously labelled with a fluorescent material with a laser beam of only one interference fringe among interference laser beams having a fringe pattern perpendicular to a flow axis along which said chromosomes or cells flow; measuring fluorescence intensity emitted from the fluorescent material on said chromosomes or cells; comparing a fluorescent profile-pattern obtained from variation of said fluorescent intensity with time with preset standard fluorescent profile-patterns of chromosome or cell; and sorting said chromosomes or cells on the basis of the comparison of said fluorescent profile-pattern with said preset standard fluorescent profile-patterns.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-08-11 | 1996-02-10 | 1996-08-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-08-11 | 2000-02-10 | 2000-08-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-08-11 | 2004-02-10 | 2004-08-11 | $7700.00 | Window Closed / Lapsed |