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US5137695A Expired / Lapsed US ⏱️ 0.0 years remaining

Apparatus for the sequential performance of chemical processes

Assignee: City of Hope
•
Filing: 1990-02-08
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Granted: 1992-08-11
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Expires: 2010-02-08
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Abstract

Abstract PCT No. PCT/US90/00751 Sec. 371 Date Sep. 28, 1990 Sec. 102(e) Date Sep. 28, 1990 PCT Filed Feb. 8, 1990 PCT Pub. No. WO90/09595 PCT Pub. Date Aug. 23, 1990.A compact apparatus having a gravity assisted, substantially linear, vertical fluid flow path through vertically aligned elements for the N-terminal and C-terminal sequencing of proteins and peptides is disclosed.

Inventors

  • 👤 Miro Rusnak
  • 👤 John E. Shively
  • 👤 Jimmy R. Calaycay

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-10 02:35
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; C; C07; C07K; C07K1/00; C07K1/107; B; B01; B01J; B01J19/00; B01J19/0046; G01N1/00; G01N1/28; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00281; B01J2219/00286; B01J2219/00351; B01J2219/00389; B01J2219/00497; B01J2219/00583; B01J2219/0059; B01J2219/00718; B01J2219/0072; B01J2219/00725; C40; C40B; C40B40/00; C40B40/04; C40B40/10; C40B60/00; C40B60/14

Claims Analysis

Total Claims: 3
Claim 1
Independent Claim 204 words
A polypeptide sequencer having a gravity assisted, substantially linear, vertical fluid flow path through vertically aligned sequence elements, said elements comprising: (i) a delivery valve means for introducing fluids into a sequencing reaction chamber, said delivery valve means comprising: a valve body, a substantially linear, vertical fluid flow passage extending through said valve body from an inlet on a first surface of said valve body to an outlet on a second surface of said valve body, said outlet being connected to a sequencing reaction chamber inlet; (ii) a sequencing reaction chamber positioned below said delivery valve means, said sequencing reaction chamber comprising a substantially linear, vertical fluid flow passage extending from said sequencing reaction chamber inlet connected to said delivery valve outlet to an outlet; (iii) a transfer valve positioned below said reaction chamber, said transfer valve comprising a substantially linear, vertical fluid flow passage extending from a inlet transfer valve connected to said outlet of said fluid flow passage of said reaction chamber to an outlet; and (iv) a conversion flask positioned below said transfer valve, said conversion flask comprising a substantially linear, fluid flow passage extending from a conversion flask inlet connected to said outlet of said transfer valve to an outlet.
Claim 2
Dependent Claim 204 words
A polypeptide sequencer having a gravity assisted, substantially linear, vertical fluid flow path through vertically aligned sequence elements, said elements comprising: (i) a delivery valve means for introducing fluids into a sequencing reaction chamber, said delivery valve means comprising: a valve body, a substantially linear, vertical fluid flow passage extending through said valve body from an inlet on a first surface of said valve body to an outlet on a second surface of said valve body, said outlet being connected to a sequencing reaction chamber inlet; (ii) a sequencing reaction chamber positioned below said delivery valve means, said sequencing reaction chamber comprising a substantially linear, vertical fluid flow passage extending from said sequencing reaction chamber inlet connected to said delivery valve outlet to an outlet; (iii) a transfer valve positioned below said reaction chamber, said transfer valve comprising a substantially linear, vertical fluid flow passage extending from a inlet transfer valve connected to said outlet of said fluid flow passage of said reaction chamber to an outlet; and (iv) a conversion flask positioned below said transfer valve, said conversion flask comprising a substantially linear, fluid flow passage extending from a conversion flask inlet connected to said outlet of said transfer valve to an outlet.
Claim 3
Dependent Claim 52 words
A polypeptide sequencer as defined by claim 1 further comprising: a selector valve positioned below said conversion flask, said selector valve comprising a substantially linear, vertical fluid flow passage extending from a inlet selector valve connected to said outlet of said conversion flask to means for conducting fluids to an analytical instrument.

References Cited (Backward Citations)

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US3175801A Cited
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USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1995-08-11 1996-02-10 1996-08-11 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1999-08-11 2000-02-10 2000-08-11 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2003-08-11 2004-02-10 2004-08-11 $7700.00 Window Closed / Lapsed

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