US5126241A
Expired / Lapsed
US
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Process for the determination of a specifically bindable substance
Assignee: Roche Diagnostics GmbH
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Filing: 1989-10-10
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Granted: 1992-06-30
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Expires: 2009-10-10
Abstract
Abstract The present invention provides a process for the determination of a specifically bindable substance by incubation of a sample solution with at least three receptors R 1 , R 2 and R 3 , of which R 1 is specifically bindable with R 2 and R 3 , as well as with the substance to be determined, R 2 brings about the binding to a solid phase and R 3 carries a labelling, separation of bound labelling from unbound labelling and measurement of the labelling in one of the two phases, wherein, as receptor R 1 , a receptor is used which has at least two binding positions which bind specifically with an epitope of the substance to be determined, as R 2 a conjugate of a partner P 1 of a specifically binding pair and of a substance S which corresponds to the substance to be determined or is a derivative thereof and has at least one epitope of the substance to be determined, the partner P 1 thereby either being bound to a solid phase or being immobilized, and as R 3 a complex is used which contains at least the substance S and a labelling.
Inventors
- 👤 Roland Schenk
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 14:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/74; G01N33/78; G01N33/543; G01N33/54306
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
177 words
Method for determination of a specifically bindable substance comprising: incubating a liquid sample with at least three receptors R 1 , R 2 and R 3 , wherein (a) R 1 specifically binds with R 2 , R 3 and said specifically bindable substance and is an antibody or an Fab 2 fragment; (b) R 2 comprises a conjugate of (i) a substance S which is identical to the specifically bindable substance to be determined and (ii) a first partner P 1 of a binding pair, (c) R 3 comprises a conjugate of S and a label, to form complexes containing R 1 , R 2 and R 3 , (d) adding a second partner of a specific binding pair P 2 to said liquid sample together with a solid phase, wherein P 2 binds to said solid phase and to P 1 to immobilize said complexes containing R 1 , R 2 and R 3 , and, determining R 3 bound to solid phase or in liquid phase as a determination of said specifically bindable substance.
Claim 2
Dependent Claim
177 words
Method for determination of a specifically bindable substance comprising: incubating a liquid sample with at least three receptors R 1 , R 2 and R 3 , wherein (a) R 1 specifically binds with R 2 , R 3 and said specifically bindable substance and is an antibody or an Fab 2 fragment; (b) R 2 comprises a conjugate of (i) a substance S which is identical to the specifically bindable substance to be determined and (ii) a first partner P 1 of a binding pair, (c) R 3 comprises a conjugate of S and a label, to form complexes containing R 1 , R 2 and R 3 , (d) adding a second partner of a specific binding pair P 2 to said liquid sample together with a solid phase, wherein P 2 binds to said solid phase and to P 1 to immobilize said complexes containing R 1 , R 2 and R 3 , and, determining R 3 bound to solid phase or in liquid phase as a determination of said specifically bindable substance.
Claim 3
Dependent Claim
152 words
Method for determination of a specifically bindable substance, comprising: incubating a liquid sample with at least three receptors R 1 , R 2 and R 3 , and with a solid phase bound partner P 2 of a binding pair, wherein: a) R 1 specifically binds with R 2 , R 3 and said specifically bindable substance and is an antibody or an Fab 2 fragment; (b) R 2 comprises a conjugate of (i) a substance S which is identical to the specifically bindable substance to be determined and (ii) a first partner P 1 of a binding pair; (c) R 3 comprises a conjugate of S and a label, (d) P 2 binds to P 1 , to form immobilized complexes containing R 1 , R 2 and R 3 , and; determining R 3 bound to solid phase or in liquid phase as a determination of said specifically bindable substance.
Claim 4
Dependent Claim
152 words
Method for determination of a specifically bindable substance, comprising: incubating a liquid sample with at least three receptors R 1 , R 2 and R 3 , and with a solid phase bound partner P 2 of a binding pair, wherein: a) R 1 specifically binds with R 2 , R 3 and said specifically bindable substance and is an antibody or an Fab 2 fragment; (b) R 2 comprises a conjugate of (i) a substance S which is identical to the specifically bindable substance to be determined and (ii) a first partner P 1 of a binding pair; (c) R 3 comprises a conjugate of S and a label, (d) P 2 binds to P 1 , to form immobilized complexes containing R 1 , R 2 and R 3 , and; determining R 3 bound to solid phase or in liquid phase as a determination of said specifically bindable substance.
Claim 5
Dependent Claim
20 words
Method of claim 2, wherein P 2 is bound to said solid phase via solid phase bound P 1 .
Claim 6
Dependent Claim
33 words
Method of claim 2, comprising adding said solid phase bound member of a specific binding pair P 2 after addition of R 1 , R 2 and R 3 to said sample solution.
Claim 7
Dependent Claim
20 words
Method of claim 1, wherein P 1 is biotin and P 2 is streptavidin, avidin or a biotin specific antibody.
Claim 8
Dependent Claim
20 words
Method of claim 2, wherein P 1 is biotin and P 2 is streptavidin, avidin or a biotin specific antibody.
Claim 9
Dependent Claim
13 words
Method of claim 1, wherein R 3 consists of S and a label.
Claim 10
Dependent Claim
13 words
Method of claim 2, wherein R 3 consists of S and a label.
Claim 11
Dependent Claim
12 words
Method of claim 1, wherein said label is radioactive chemiluminescent, or fluorescent.
Claim 12
Dependent Claim
12 words
Method of claim 2, wherein said label is radioactive chemiluminescent, or fluorescent.
Claim 13
Dependent Claim
41 words
Method of claim 1, wherein R 3 comprises a first member complex consisting of S and P 1 and a second member complex consisting of P 2 and a label, wherein P 1 and P 2 are bound to each other.
Claim 14
Dependent Claim
41 words
Method of claim 2, wherein R 3 comprises a first member complex consisting of S and P 1 and a second member complex consisting of P 2 and a label, wherein P 1 and P 2 are bound to each other.
Claim 15
Dependent Claim
32 words
Method of claim 1, wherein P 1 and P 2 are selected from the group consisting of biotin-streptavidin, biotin-avidin, biotin-biotin specific antibody, antigen-antigen specific antibody, hapten-hapten binding protein and oligopeptide-oligopeptide specific antibody.
Claim 16
Dependent Claim
32 words
Method of claim 2, wherein P 1 and P 2 are selected from the group consisting of biotin-streptavidin, biotin-avidin, biotin-biotin specific antibody, antigen-antigen specific antibody, hapten-hapten binding protein and oligopeptide-oligopeptide specific antibody.
Claim 17
Dependent Claim
25 words
Method of claim 1, wherein said solid phase is a matrix consisting of a synthetic resin and P 2 is bound thereto via a spacer.
Claim 18
Dependent Claim
25 words
Method of claim 2, wherein said solid phase is a matrix consisting of a synthetic resin and P 2 is bound thereto via a spacer.
Claim 19
Dependent Claim
16 words
Method of claim 1, wherein P 1 is biotin and P 2 is avidin or streptavidin.
Claim 20
Dependent Claim
16 words
Method of claim 2, wherein P 1 is biotin and P 2 is avidin or streptavidin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-06-30 | 1995-12-30 | 1996-06-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-06-30 | 1999-12-30 | 2000-06-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-06-30 | 2003-12-30 | 2004-06-30 | $7700.00 | Window Closed / Lapsed |