US5124358A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Effect of transglutaminase inhibition on microfilariae development and macrofilariae viability
Assignee: University of South Florida
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Filing: 1990-01-16
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Granted: 1992-06-23
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Expires: 2010-01-16
Abstract
Abstract A method is described for blocking maturation and production of microfilariae in adult filarial nematodes utilizing a transglutaminase inhibitor, such as monodansyl cadaverine (MDC). Experiments applying this method to several Brugia filarial infections, including Brugia malayi, were successful. Higher concentration of transglutaminase inhibitor proved to be filariacidal.
Inventors
- 👤 Mehta Kapil
- 👤 Undaru R. Rao
- 👤 Ann C. Vickery
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:21
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/33; A61K31/395; A61K31/41; A61K31/415; A61K31/13; A61K31/14; A61K31/16; A61K31/18; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
46 words
A method for inhibiting maturation and production of microfilariae by female filarial nematodes residing in a mammalian host comprising administering to a mammalian host monodansyl cadaverine, methylamine, histamine, or putrescine in an amount inhibiting transglutaminase activity and maturation and microfilariae production of resident female filarial nematodes.
Claim 2
Independent Claim
46 words
A method for inhibiting maturation and production of microfilariae by female filarial nematodes residing in a mammalian host comprising administering to a mammalian host monodansyl cadaverine, methylamine, histamine, or putrescine in an amount inhibiting transglutaminase activity and maturation and microfilariae production of resident female filarial nematodes.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein the filarial nematode is Brugia pahangi, Brugia patei, or Brugia timori.
Claim 4
Dependent Claim
12 words
The method of claim 1 wherein the filarial nematode is Wuchereria bancrofti.
Claim 5
Dependent Claim
12 words
The method of claim 1 wherein the filarial nematode is Loa loa.
Claim 6
Dependent Claim
12 words
The method of claim 1 wherein the filarial nematode is Onchocerca volvulus.
Claim 7
Dependent Claim
25 words
The method of claim 1 wherein the amount of monodansyl cadaverine, methylamine, histamine, or putrescine results in about a 200 micromolar concentration in the host.
Claim 8
Independent Claim
53 words
A method for inhibiting maturation and production of microfilariae by Brugia malayi residing in a mammalian host comprising treating an adult female Brugia malayi nematode in said mammalian host with monodansyl cadaverine, methylamine, histamine, or putrescine in an amount sufficient to inhibit transglutaminase activity, and maturation and microfilariae production of said Brugia malayi.
Claim 9
Independent Claim
53 words
A method for inhibiting maturation and production of microfilariae by Brugia malayi residing in a mammalian host comprising treating an adult female Brugia malayi nematode in said mammalian host with monodansyl cadaverine, methylamine, histamine, or putrescine in an amount sufficient to inhibit transglutaminase activity, and maturation and microfilariae production of said Brugia malayi.
Claim 10
Independent Claim
12 words
The method of claims 1 or 3 wherein monodansyl cadaverine is administered.
Claim 11
Independent Claim
52 words
The method for inhibiting in vitro maturation and production of microfilariae by a Brugia malayi, Brugia pahangi or Brugia patei nematode comprising treating an adult female Brugia malayi, Brugia pahangi or Brugia patei nematode with monodansyl cadaverine, methylamine, histamine, or putrescine in an amount sufficient to inhibit transglutaminase activity of said nematode.
Claim 12
Independent Claim
52 words
The method for inhibiting in vitro maturation and production of microfilariae by a Brugia malayi, Brugia pahangi or Brugia patei nematode comprising treating an adult female Brugia malayi, Brugia pahangi or Brugia patei nematode with monodansyl cadaverine, methylamine, histamine, or putrescine in an amount sufficient to inhibit transglutaminase activity of said nematode.
Claim 13
Dependent Claim
13 words
The method of claim 9 wherein the nematode is treated with monodansyl cadaverine.
Claim 14
Independent Claim
33 words
A method for treating lymphatic filariasis in a mammalian host comprising administering monodansyl cadaverine, methylamine, histamine or putrescine to said host in an amount sufficient to kill adult filarial nematodes producing lymphatic filariasis.
Claim 15
Independent Claim
33 words
A method for treating lymphatic filariasis in a mammalian host comprising administering monodansyl cadaverine, methylamine, histamine or putrescine to said host in an amount sufficient to kill adult filarial nematodes producing lymphatic filariasis.
Claim 16
Independent Claim
39 words
A method for treating lymphatic filariasis produced by Brugia malayi in a mammalian host comprising administering monodansyl cadaverine, methylamine, histamine or putrescine to said host in an amount sufficient to kill adult Brugia malayi nematodes producing the lymphatic filariasis.
Claim 17
Independent Claim
39 words
A method for treating lymphatic filariasis produced by Brugia malayi in a mammalian host comprising administering monodansyl cadaverine, methylamine, histamine or putrescine to said host in an amount sufficient to kill adult Brugia malayi nematodes producing the lymphatic filariasis.
Claim 18
Independent Claim
23 words
The method of claims 1, 11 or 12 wherein the monodansyl cadaverine, methylamine, histamine or putrescine is parenterally administered to a mammalian host.
Claim 19
Independent Claim
23 words
The method of claims 1, 11 or 12 wherein the monodansyl cadaverine, methylamine, histamine or putrescine is administered to a mammalian host enterally.
Claim 20
Dependent Claim
24 words
The method of claim 11 wherein the adult filarial nematode producing the lymphatic filariasis is Brugia pahangi, Brugia patei, Brugia timori or Wuchereria bancrofti.
Claim 21
Independent Claim
14 words
The method of claims 11 or 12 wherein the transglutaminase inhibitor is monodansyl cadaverine.
Claim 22
Independent Claim
15 words
The method of claims 1, 11 or 12 wherein the mammalian host is a rodent.
Claim 23
Independent Claim
15 words
The method of claims 1, 11 or 12 wherein the mammalian host is a human.
References Cited (Backward Citations)
US4396628A
Cited
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