US5087438A — Method for affecting intracellular and extracellu…

Claim 1

Independent Claim 350 words

A process for the treatment of diseased cells in at least one region in the tissue of a host organism containing said diseased cells and living normal cells without substantially damaging said living normal cells comprising: providing to said host organism minute particles less than about 1 micron capable of being taken up by said diseased cells; selecting said particles to affect intracellular and extracellular events for the enhancement of treating infectious diseases produced by organisms selected from the group consisting of Salmonella, Klebsiella, Escherichia, Clostridium Mycobacterium, Pseudomonas, Peptostreptococcus, Phycomyces, Candida, Ustilago, Entamoeba, Trypanosoma, Leishmania and RNA viruses by the selection of said particles; allowing said particles to effect at least one event comprising intracellular events and membrane events in said tissue; subjecting said organism to a relatively low frequency alternating, oscillating, or pulsed electromagnetic field to provide energy to said diseased cells and selectively heat said diseased cells wherein said particles are selected from the group consisting of: a) cobalt, zinc, iron, chromium, nickel, platinum, rare earth metals and compounds selected from the group consisting of dysprosium, erbium, europium, gadolinium, holmium, samarium, terbium, thulium, ytterbium, yttrium; dysprosium sulfate, erbium sulfate, europium oxide, europium sulfate, gadolinium oxide, gadolinium sulfate, holmium oxide, samarium sulfate, terbium sulfate, thulium oxide, ytterbium sulfide, yttrium oxide, yttrium sulfate, yttrium ferrioxide (Y 3 Fe 5 O 12 ), yttrium oxide (Y 3 Al 5 O 12 ), dysprosium-nickel, dysprosium-cobalt, gadolinium-iron, ytterbium-iron, cobalt-samarium, gadolinium-ytterbium, dysprosium-gallium, and actinide series elements and compounds thereof; b) dextran metal complexes wherein said metal is selected from the group consisting of cobalt, zinc, chromium, iron, gallium, manganese, nickel, platinum, dysprosium, erbium, europium, gadolinium, holmium, samarium, terbium, thulium, ytterbium, yttrium, dysprosium-nickel, dysprosium-cobalt, gadolinium-iron, ytterbium-iron, cobalt-samarium, gadolinium-yttrium, and dysprosium-gallium; c) iron transporting and chelating compounds selected from the group consisting of ferric ammonium citrate, enterochelin, transferrin, metallothionein, hydroxamates, phenolates, ferrichromes, desferriferrichromes, ferritin, ferric mycobactins, ferredoxin and rubredoxin; d) porphyrins selected from the group consisting of etioporphyrins, meso-porphyrins, uroporphyrins, coproporphyrins, protoporphyrins, dicarboxylic acid containing porphyrins, tetraphenylporphyrin sulfonate, hematoporphyrins, chlorophylls, and cytochromes; e) and combinations of the materials of said sub-paragraphs a), b), c) and d).

Claim 2

Dependent Claim 350 words

A process for the treatment of diseased cells in at least one region in the tissue of a host organism containing said diseased cells and living normal cells without substantially damaging said living normal cells comprising: providing to said host organism minute particles less than about 1 micron capable of being taken up by said diseased cells; selecting said particles to affect intracellular and extracellular events for the enhancement of treating infectious diseases produced by organisms selected from the group consisting of Salmonella, Klebsiella, Escherichia, Clostridium Mycobacterium, Pseudomonas, Peptostreptococcus, Phycomyces, Candida, Ustilago, Entamoeba, Trypanosoma, Leishmania and RNA viruses by the selection of said particles; allowing said particles to effect at least one event comprising intracellular events and membrane events in said tissue; subjecting said organism to a relatively low frequency alternating, oscillating, or pulsed electromagnetic field to provide energy to said diseased cells and selectively heat said diseased cells wherein said particles are selected from the group consisting of: a) cobalt, zinc, iron, chromium, nickel, platinum, rare earth metals and compounds selected from the group consisting of dysprosium, erbium, europium, gadolinium, holmium, samarium, terbium, thulium, ytterbium, yttrium; dysprosium sulfate, erbium sulfate, europium oxide, europium sulfate, gadolinium oxide, gadolinium sulfate, holmium oxide, samarium sulfate, terbium sulfate, thulium oxide, ytterbium sulfide, yttrium oxide, yttrium sulfate, yttrium ferrioxide (Y 3 Fe 5 O 12 ), yttrium oxide (Y 3 Al 5 O 12 ), dysprosium-nickel, dysprosium-cobalt, gadolinium-iron, ytterbium-iron, cobalt-samarium, gadolinium-ytterbium, dysprosium-gallium, and actinide series elements and compounds thereof; b) dextran metal complexes wherein said metal is selected from the group consisting of cobalt, zinc, chromium, iron, gallium, manganese, nickel, platinum, dysprosium, erbium, europium, gadolinium, holmium, samarium, terbium, thulium, ytterbium, yttrium, dysprosium-nickel, dysprosium-cobalt, gadolinium-iron, ytterbium-iron, cobalt-samarium, gadolinium-yttrium, and dysprosium-gallium; c) iron transporting and chelating compounds selected from the group consisting of ferric ammonium citrate, enterochelin, transferrin, metallothionein, hydroxamates, phenolates, ferrichromes, desferriferrichromes, ferritin, ferric mycobactins, ferredoxin and rubredoxin; d) porphyrins selected from the group consisting of etioporphyrins, meso-porphyrins, uroporphyrins, coproporphyrins, protoporphyrins, dicarboxylic acid containing porphyrins, tetraphenylporphyrin sulfonate, hematoporphyrins, chlorophylls, and cytochromes; e) and combinations of the materials of said sub-paragraphs a), b), c) and d).

Claim 3

Dependent Claim 286 words

A process comprising selecting particles of less than about 1 micron to affect intracellular and extracellular events for the enhancement of Genetic Engineering processes or for DNA-RNA modifications by the selection of said particles comprising, introducing said particles into a host organism and subjecting said host organism to a relatively low frequency alternating, oscilating or pulsed electromagnetic field to provide energy to said organism and enhance said Genetic Engineering process wherein said particles are selected from the group consisting of: a) cobalt, zinc, iron, chromium, nickel, platinum, rare earth metals and compounds selected from the group consisting of dysprosium, erbium, europium, gadolinium, holmium, samarium, terbium, thulium, ytterbium, yttrium; dysprosium sulfate, erbium sulfate, europium oxide, europium sulfate, gadolinium oxide, gadolinium sulfate, holmium oxide, samarium sulfate, terbium sulfate, thulium oxide, ytterbium sulfide, yttrium oxide, yttrium sulfate, yttrium ferrioxide (Y 3 Fe 5 O 12 ), yttrium oxide (Y 3 Al 5 O 12 ), dysprosium-nickel, dysprosium-cobalt, gadolinium-iron, ytterbium-iron, cobalt-samarium, gadolinium-ytterbium, dysprosium-gallium, and actinide series elements and compounds thereof; b) dextran metal complexes wherein said metal is selected from the group consisting of cobalt, zinc, chromium, iron, gallium, manganese, nickel, platinum, dysprosium, erbium, europium, gadolinium, holmium, samarium, terbium, thulium, ytterbium, yttrium, dysprosium-nickel, dysprosium-cobalt, gadolinium-iron, ytterbium-iron, cobalt-samarium; gadolinium-yttrium, and dysprosium-gallium, Fe 3 O 4 Fe 2 O 3 , Fe 2 O 3 , FeOOH: c) iron transporting and chelating compounds selected from the group consisting of ferric ammonium citrate, enterochelin, transferrin, metallothionein, hydroxamates, phenolates, ferrichromes, desferriferrichromes, ferritin, ferric mycobactins, ferredoxin and rubredoxin; d) porphyrins selected from the group consisting of etioporphyrins, meso-porphyrins, uroporphyrins, coproporphyrins, protoporphyrins, dicarboxylic acid containing porphyrins, tetraphenylporphyrin sulfonate, hematoporphyrins, chlorophylls, and cytochromes; e) and combinations of the materials of said sub-paragraphs a), b), c) and d).

Claim 4

Dependent Claim 286 words

A process comprising selecting particles of less than about 1 micron to affect intracellular and extracellular events for the enhancement of Genetic Engineering processes or for DNA-RNA modifications by the selection of said particles comprising, introducing said particles into a host organism and subjecting said host organism to a relatively low frequency alternating, oscilating or pulsed electromagnetic field to provide energy to said organism and enhance said Genetic Engineering process wherein said particles are selected from the group consisting of: a) cobalt, zinc, iron, chromium, nickel, platinum, rare earth metals and compounds selected from the group consisting of dysprosium, erbium, europium, gadolinium, holmium, samarium, terbium, thulium, ytterbium, yttrium; dysprosium sulfate, erbium sulfate, europium oxide, europium sulfate, gadolinium oxide, gadolinium sulfate, holmium oxide, samarium sulfate, terbium sulfate, thulium oxide, ytterbium sulfide, yttrium oxide, yttrium sulfate, yttrium ferrioxide (Y 3 Fe 5 O 12 ), yttrium oxide (Y 3 Al 5 O 12 ), dysprosium-nickel, dysprosium-cobalt, gadolinium-iron, ytterbium-iron, cobalt-samarium, gadolinium-ytterbium, dysprosium-gallium, and actinide series elements and compounds thereof; b) dextran metal complexes wherein said metal is selected from the group consisting of cobalt, zinc, chromium, iron, gallium, manganese, nickel, platinum, dysprosium, erbium, europium, gadolinium, holmium, samarium, terbium, thulium, ytterbium, yttrium, dysprosium-nickel, dysprosium-cobalt, gadolinium-iron, ytterbium-iron, cobalt-samarium; gadolinium-yttrium, and dysprosium-gallium, Fe 3 O 4 Fe 2 O 3 , Fe 2 O 3 , FeOOH: c) iron transporting and chelating compounds selected from the group consisting of ferric ammonium citrate, enterochelin, transferrin, metallothionein, hydroxamates, phenolates, ferrichromes, desferriferrichromes, ferritin, ferric mycobactins, ferredoxin and rubredoxin; d) porphyrins selected from the group consisting of etioporphyrins, meso-porphyrins, uroporphyrins, coproporphyrins, protoporphyrins, dicarboxylic acid containing porphyrins, tetraphenylporphyrin sulfonate, hematoporphyrins, chlorophylls, and cytochromes; e) and combinations of the materials of said sub-paragraphs a), b), c) and d).

Claim 5

Dependent Claim 181 words

A process for the treatment of diseased cells in at least one region in the tissue of a host organism containing said diseased cells and living normal cells without substantially damaging said living normal cells comprising: providing to said host organism minute particles less than about 1 micron capable of being taken up by said diseased cells; selecting said particles to affect intracellular and extracellular events for the enhancement of treating infectious diseases produced by organisms selected from the group consisting of Salmonella, Klebsiella, Escherichia, Clostrididium, Mycobacterium, Pseudomonas, Peptostreptococcus, Phycomyces, Candida, Ustilago, Entamoeba, Trypanosoma, Leishmania and RNA viruses by the selection of said particles; allowing said particles to effect at least one event comprising intracellular events and membrane events in said tissue; subjecting said organism to a relatively low frequency alternating, oscillating, or pulsed electromagnetic field to provide energy to said diseased cells and selectively heat said diseased cells wherein said particles are selected from the group consisting of: a) ferromagnetic, paramagnetic and diamagnetic elements, inorganic compounds, organic compounds and combinations thereof,metalloporphyrins, Fe 2 O 3 , FeOOH, and metal metalloporphyrins.

Claim 6

Dependent Claim 181 words

A process for the treatment of diseased cells in at least one region in the tissue of a host organism containing said diseased cells and living normal cells without substantially damaging said living normal cells comprising: providing to said host organism minute particles less than about 1 micron capable of being taken up by said diseased cells; selecting said particles to affect intracellular and extracellular events for the enhancement of treating infectious diseases produced by organisms selected from the group consisting of Salmonella, Klebsiella, Escherichia, Clostrididium, Mycobacterium, Pseudomonas, Peptostreptococcus, Phycomyces, Candida, Ustilago, Entamoeba, Trypanosoma, Leishmania and RNA viruses by the selection of said particles; allowing said particles to effect at least one event comprising intracellular events and membrane events in said tissue; subjecting said organism to a relatively low frequency alternating, oscillating, or pulsed electromagnetic field to provide energy to said diseased cells and selectively heat said diseased cells wherein said particles are selected from the group consisting of: a) ferromagnetic, paramagnetic and diamagnetic elements, inorganic compounds, organic compounds and combinations thereof,metalloporphyrins, Fe 2 O 3 , FeOOH, and metal metalloporphyrins.

Claim 7

Dependent Claim 102 words

A process comprising selecting particles of less than about 1 micron to affect intracellular and extracellular events for the enhancement of Genetic Engineering processes or for DNA-RNA modifications by the selection of said particles comprising, introducing said particles into a host organism and subjecting said host organism to a relatively low frequency alternating, oscilating or pulsed electromagnetic field to provide energy to said organism and enhance said Genetic Engineering process wherein said particles are selected from the group consisting of: a) paramagnetic and diamagnetic elements, inorganic compounds, organic compounds and combinations thereof, metalloporphyrins, Fe 2 O 3 , FeOOH, and metal metalloporphyrins.

Claim 8

Dependent Claim 102 words

A process comprising selecting particles of less than about 1 micron to affect intracellular and extracellular events for the enhancement of Genetic Engineering processes or for DNA-RNA modifications by the selection of said particles comprising, introducing said particles into a host organism and subjecting said host organism to a relatively low frequency alternating, oscilating or pulsed electromagnetic field to provide energy to said organism and enhance said Genetic Engineering process wherein said particles are selected from the group consisting of: a) paramagnetic and diamagnetic elements, inorganic compounds, organic compounds and combinations thereof, metalloporphyrins, Fe 2 O 3 , FeOOH, and metal metalloporphyrins.

Claim 9

Dependent Claim 26 words

The process of claims 1, 2, 3 or 4 wherein said alternating, oscillating and/or pulsed electromagnetic field is between about 1 Hz to about 100 MHz.

Claim 10

Dependent Claim 33 words

The process of claims 1, 2, 3, or 4 wherein said alternating, oscillating and/or pulsed electromagnetic field is produced by apparatus comprising a coil, capacitor plates or electrodes in the said host organism.

Claim 11

Dependent Claim 37 words

The process of claims 1, 2, 3 or 4 wherein said particles are selected to affect the intracellular conductivity, dielectric properties, charge accumulation on the membrane, membrane conductance, membrane capacitance and the electric dipole environment of cells.

Claim 12

Dependent Claim 58 words

The process of claims 1, 2, 3 or 4 wherein the natural occurring metal moiety of said porphyrin is optionally substituted with a metal selected from the group comprising cobalt, zinc, chromium, gallium, iron, manganese, nickel, platinum, dyprosium, erbium, europium, gadolinium, holmium, samarium, terbium, thulium, ytterbium, yttrium, dyprosium-nickel, dyprosium-cobalt, gadolinium-iron, yttrebium-iron, cobalt-samarium, gadolinium-yttrium, and dyprosium-gallium; and combinations thereof.

Claim 13

Dependent Claim 24 words

The process according to claims 1, 2, 3 or 4 wherein said iron transporting, iron chelating and porphyrin compounds are chemically complexed with dextran.

Claim 14

Dependent Claim 19 words

The process according to claims 1, 2, 3 or 4 wherein said compound is chemically complexed with an antibody.

Claim 15

Dependent Claim 45 words

The process of claims 1, 2, 3 or 4 for heating said host organism comprising decreasing the power level at a given frequency of said field by altering intracellular environment and membrane characteristics in said tissue by the provision of said particles to said host.

Claim 16

Dependent Claim 41 words

The process of claims 1, 2, 3 or 4 for heating said diseased cells comprising decreasing the frequency of said field by altering the intracellular environment and membrane characteristics in said tissue by the provision of said particles to said host.

Claim 17

Dependent Claim 44 words

The process of claims 1, 2, 3 or 4 comprising applying a localized static magnetic field to said host organism to aid in the intracellular uptake and energy absorption of the electric or magnetic dipoles either introduced or already present in said host organism.

Claim 18

Dependent Claim 59 words

The process of claims 1, 2, 3 or 4 wherein a localized static magnetic or electric filed is applied to said host organism after providing said particles to said host organisms but prior to or during the application of said alternating, oscillating and/or pulsed electromagnetic field to enhance the intracellular of energy and the energy-absorption responsiveness of said particles.

Claim 19

Dependent Claim 56 words

The process of claim 13 wherein a localized static magnetic or electric field is applied to said host organism prior to and/or during the application of said alternating, oscillating and/or pulsed electromagnetic field to enhance the intracellular energy uptake and energy absorption of the electric or magnetic dipoles either introduced or already present in said tissue.

Claim 20

Dependent Claim 19 words

The process of claim 13 wherein the static magnetic or electrical field is between 100 gauss and 80 kilogauss.

Claim 21

Dependent Claim 39 words

The process of claims 1 or 3 wherein said particles are introduced into the extracellular environment of said tissue to alter membrane events and potentiate energy delivery to said diseased cells and/or reduce energy delivery to said normal cells.

Claim 22

Dependent Claim 17 words

The process of claims 1 or 3 wherein particles are introduced intravenously, intra-arterially, intra-lymphatically, and or locally.

Claim 23

Dependent Claim 49 words

The process of claims 1, 2, 3 or 4 wherein an alternating electromagnetic filed between 1 Hz and 500 MHz is used to affect said particles and make them more or less responsive to an exciting alternating electromagnetic field produced by magnetostrictive induced vibrations applied to said host organism.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1995-02-10	1995-08-12	1996-02-11	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	1999-02-10	1999-08-12	2000-02-11	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	2003-02-10	2003-08-12	2004-02-11	$7700.00	Window Closed / Lapsed

Method for affecting intracellular and extracellular electric and magnetic dipoles

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