Patent US2054049A successfully refreshed from Google Patents.
US5079142A
Expired / Lapsed
US
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Orthogonal flow immunoassays and devices
Assignee: Synbiotics LLC
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Filing: 1987-01-23
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Granted: 1992-01-07
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Expires: 2007-01-23
Abstract
Abstract Methods and composition are provided for diagnostic assays, where a sample traverses a bibulous matrix in a first plane and the flow path redirected into a second plane at a binding site, where a signal is created in relation to the presence of analyte. Various protocols may be employed where components of a signal producing system may be combined with the sample, the matrix, or added at the binding site.
Inventors
- 👤 Patrick F. Coleman
- 👤 Edward T. Maggio
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 06:03
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; G01N33/54389; Y; Y10; Y10S; Y10S435/00; Y10S435/97
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
135 words
A device for the detection of an analyte comprising: a matrix in a first plane providing for capillary transport of an aqueous sample medium; cover means positioned over said matrix, a first port in said cover means, with said first port positioned over said matrix for directing said sample medium to a first site on said matrix; flow means in contact with said matrix at a second site on said matrix for directing flow of said sample medium at said second site to a second plane substantially parallel to said matrix in said first plane, said flow through said flow means being substantially perpendicular to said matrix; a immunoglobulin bound to said matrix at said second site; and absorbant means, in contact with said flow means, for receiving said sample medium in said second plane.
Claim 2
Independent Claim
135 words
A device for the detection of an analyte comprising: a matrix in a first plane providing for capillary transport of an aqueous sample medium; cover means positioned over said matrix, a first port in said cover means, with said first port positioned over said matrix for directing said sample medium to a first site on said matrix; flow means in contact with said matrix at a second site on said matrix for directing flow of said sample medium at said second site to a second plane substantially parallel to said matrix in said first plane, said flow through said flow means being substantially perpendicular to said matrix; a immunoglobulin bound to said matrix at said second site; and absorbant means, in contact with said flow means, for receiving said sample medium in said second plane.
Claim 3
Dependent Claim
33 words
A device according to claim 1, including a second port in said cover situated over said second site for directing fluids through said second site and said flow means to said absorbant means.
Claim 4
Dependent Claim
44 words
A device according to claim 2, including directing means on a separator support, said separator support substantially parallel to and under said matrix, said directing means in contact with said matrix for linearly directing sample medium from said first site to said second site.
Claim 5
Dependent Claim
27 words
A device according to claim 3, wherein said directing means includes a raised lip from said separator support to direct said sample medium to said second site.
Claim 6
Dependent Claim
28 words
A device according to claim 3, including spring means for urging said matrix, said flow means and said absorbant toward each other in order to maintain intimate contact.
Claim 7
Dependent Claim
105 words
A device according to claim 1, including at least one of: (a) a second port in said cover means, said second port situated over said second site for directing fluids through said second site and said flow means to said absorbant means; (b) direction means on a separator support, said separator support substantially parallel to and under said matrix, said directing means in contact with said matrix for linearly directing said sample medium from said first site to said second site; or (c) spring means for urging said matrix, said flow means and said absorbant means toward each other in order to maintain intimate contact.
Claim 8
Dependent Claim
25 words
A device according to claim 1, including a first receptacle on said cover means, said receptacle having an open bottom communicating with said first port.
Claim 9
Dependent Claim
54 words
A device according to claim 7, including a second port in said cover means, said second pot situated over said second site for directing fluids through said second site and said flow means to said absorbant means, and a second receptacle on said cover means having an open bottom communicating with said second port.
Claim 10
Dependent Claim
157 words
A multiunit device for the detection of at least one analyte comprising a plurality of containers, each container sharing at least one common wall with an adjacent container, each container comprising: a matrix in a first plane providing for capillary transport of an aqueous sample medium; cover means positioned over said matrix, a first port in said cover means, with said first port positioned over said matrix for direction said sample medium to a first site on said matrix; flow means in contact with said matrix at a second site on said matrix for directing flow of said sample medium at said second site to a second plane substantially parallel to said matrix in said first plane, said flow through said flow means being substantially perpendicular to said matrix; an immunoglobulin bound to said matrix at said second site; and absorbant means, in contact with said flow means, for receiving said sample medium in said second plane.
Claim 11
Dependent Claim
157 words
A multiunit device for the detection of at least one analyte comprising a plurality of containers, each container sharing at least one common wall with an adjacent container, each container comprising: a matrix in a first plane providing for capillary transport of an aqueous sample medium; cover means positioned over said matrix, a first port in said cover means, with said first port positioned over said matrix for direction said sample medium to a first site on said matrix; flow means in contact with said matrix at a second site on said matrix for directing flow of said sample medium at said second site to a second plane substantially parallel to said matrix in said first plane, said flow through said flow means being substantially perpendicular to said matrix; an immunoglobulin bound to said matrix at said second site; and absorbant means, in contact with said flow means, for receiving said sample medium in said second plane.
Claim 12
Independent Claim
146 words
A device for the detection of an analyte comprising: a container including a cover; a bibulous matrix in a first plane providing for capillary transport of an aqueous sample medium, said bibulous matrix in said container and under said cover; a first port in said cover, said first port positioned over said bibulous matrix for directing said sample medium to a first site on said matrix; flow means in contact with said matrix at a second site on said matrix for directing flow of said sample at said second site to a second plane substantially parallel to said bibulous matrix in said first plane, said flow through said flow means being substantially perpendicular to said bibulous matrix; an immunoglobulin bound to said bibulous matrix at said second site; and absorbant means, in contact with said flow means, for receiving said sample medium in said second plane.
Claim 13
Independent Claim
146 words
A device for the detection of an analyte comprising: a container including a cover; a bibulous matrix in a first plane providing for capillary transport of an aqueous sample medium, said bibulous matrix in said container and under said cover; a first port in said cover, said first port positioned over said bibulous matrix for directing said sample medium to a first site on said matrix; flow means in contact with said matrix at a second site on said matrix for directing flow of said sample at said second site to a second plane substantially parallel to said bibulous matrix in said first plane, said flow through said flow means being substantially perpendicular to said bibulous matrix; an immunoglobulin bound to said bibulous matrix at said second site; and absorbant means, in contact with said flow means, for receiving said sample medium in said second plane.
Claim 14
Dependent Claim
14 words
A device according to claim 10, wherein said immunoglobulin is an antibody to analyte.
Claim 15
Dependent Claim
15 words
A device according to claim 10, wherein and immunoglobulin is an antibody to an enzyme.
Claim 16
Dependent Claim
104 words
A device according to claim 11, including at least one of: (a) a second port in said cover means, said second port situated over said second site for directing fluids through said second site and said flow means to said absorbant means; (b) direction means on a separator support, said separator support substantially parallel to and under said matrix, said direction means in contact with said matrix for linearly direction said sample medium from said first site to said direction site; or (c) spring means for urging said bibulous matrix, said flow means and said absorbant means toward each other for making intimate contact.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-01-06 | 1995-07-08 | 1996-01-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-01-06 | 1999-07-08 | 2000-01-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-01-06 | 2003-07-08 | 2004-01-07 | $7700.00 | Window Closed / Lapsed |