US5055459A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Selective elimination of malignant cells from bone marrow by bis (acyloxy) propylphosphoramidates
Assignee: The University of Texas System
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Filing: 1989-04-06
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Granted: 1991-10-08
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Expires: 2009-04-06
Abstract
Abstract A method for purging tumor cells from bone marrow of a host, the method comprising extracting bone marrow cells from the host; treating extracted bone marrow cells with a therapeutic level of a compound having the structure: <IMAGE> wherein R is CH3, C2H5, C3H7, t-C4H9 or C6H5; R1 is NH2, NHCH3, NHC2H5, NHC3H7, NHC4H9, NHCH2CH2Cl, NHC6H5, N(CH3)2, N(C2H5)2, N(C3H7)2, NCH3(C2H5), NCH3(C3H7), N(CH2CH2Cl)2, NHOH, NHNHCO2CH2C6H5, NHNHCO2C(CH3)3, OCH3, OC2H5, OC3H7, OC4H9, OC6H5, OCH2C6H5, CH3, C2H5, C3H7, C4H9, CH2NO2 or CH2NH2; and R2 is NHCH2CH2Cl or N(CH2Ch2Cl)2. Intravascularly infusion of the treated bone marrow cells into the host then serves to reimplant tumor-free marrow cells.
Inventors
- 👤 Borje S. Andersson
- 👤 David Farquhar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:57
CPC Classifications
C; C07; C07H; C07H15/00; C07H15/20; C07H15/24; C07H15/252; C07F; C07F9/00; C07F9/02; C07F9/06; C07F9/22; C07F9/24; C07F9/2404; C07F9/2408; C07F9/2433; C07F9/245; C07F9/2454; C07F9/2458; C07F9/2479; C07F9/2491; C07F9/28; C07F9/38; C07F9/44; C07F9/4403; C07F9/4407; C07F9/4461; C07F9/4465; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
123 words
A method for purging leukemic tumor cells from bone marrow of a mammalian host, the method comprising extracting bone marrow cells from the host; treating extracted bone marrow cells, for a period of time and with a therapeutic level sufficient to kill leukemic cells, with a compound having the structure: ##STR16## wherein: R is --Ch 3 ; R 1 is --NH 2 , --NHCH 3 , --NHC 2 H 5 , --NHCH 2 CH 2 Cl, --N(CH 3 ) 2 , --N(C 2 H 5 ) 2 , --OCH 3 , or --OC 2 H 5 ; R 2 is --NHCH 2 CH 2 Cl or --N(CH 2 CH 2 Cl) 2 ; and intravascularly infusing treated bone marrow cells into the host.
Claim 2
Independent Claim
123 words
A method for purging leukemic tumor cells from bone marrow of a mammalian host, the method comprising extracting bone marrow cells from the host; treating extracted bone marrow cells, for a period of time and with a therapeutic level sufficient to kill leukemic cells, with a compound having the structure: ##STR16## wherein: R is --Ch 3 ; R 1 is --NH 2 , --NHCH 3 , --NHC 2 H 5 , --NHCH 2 CH 2 Cl, --N(CH 3 ) 2 , --N(C 2 H 5 ) 2 , --OCH 3 , or --OC 2 H 5 ; R 2 is --NHCH 2 CH 2 Cl or --N(CH 2 CH 2 Cl) 2 ; and intravascularly infusing treated bone marrow cells into the host.
Claim 3
Independent Claim
114 words
A method for purging leukemic tumor cells from bone marrow of a mammalian host, the method comprising: extracting bone marrow cells from the host; treating extracted bone marrow cells, for a period of time and with a therapeutic level sufficient to kill leukemic cells, with a compound having the structure: ##STR17## wherein: R is --Ch 3 ; R 1 is --NH 2 , --NHCH 3 , --NHC 2 H 5 , --NHCH 2 CH 2 Cl, --N(CH 3 ) 2 , --N(C 2 H 5 ) 2 , --OCH 3 , or --OC 2 H 5 ; and R 2 is --NHCH 2 CH 2 Cl or --N(CH 2 CH 2 Cl) 2 .
Claim 4
Independent Claim
114 words
A method for purging leukemic tumor cells from bone marrow of a mammalian host, the method comprising: extracting bone marrow cells from the host; treating extracted bone marrow cells, for a period of time and with a therapeutic level sufficient to kill leukemic cells, with a compound having the structure: ##STR17## wherein: R is --Ch 3 ; R 1 is --NH 2 , --NHCH 3 , --NHC 2 H 5 , --NHCH 2 CH 2 Cl, --N(CH 3 ) 2 , --N(C 2 H 5 ) 2 , --OCH 3 , or --OC 2 H 5 ; and R 2 is --NHCH 2 CH 2 Cl or --N(CH 2 CH 2 Cl) 2 .
Claim 5
Dependent Claim
16 words
The method of claim 1 or 2 wherein the leukemic tumor cells are human leukemic cells.
Claim 6
Dependent Claim
18 words
The method of claim 1 or 2 wherein the therapeutic level is from 20 ηg/ml to 200 ηg/ml.
Claim 7
Dependent Claim
15 words
The method of claim 1 or 2 wherein the therapeutic level is about 39.6 μm.
Claim 8
Dependent Claim
16 words
The method of claim 1 or 2 wherein the leukemic tumor cells are myelogenous leukemia cells.
Claim 9
Dependent Claim
16 words
The method of claim 1 or 2 wherein the tumor cells are human myelogenous leukemia cells.
Claim 10
Dependent Claim
24 words
The method of claim 1 or 2 wherein R 1 is --NH 2 and R 2 is --N(CH 2 CH 2 Cl) 2 .
Claim 11
Dependent Claim
25 words
The method of claim 1 or 2 wherein R 1 is --NHCH 2 CH 2 Cl, and R 2 is --NHCH 2 CH 2 Cl.
Claim 12
Dependent Claim
29 words
The method of claim 1 or 2 wherein R 1 is --OCH 3 or --OC 2 H 5 and R 2 is --N(CH 2 CH 2 Cl) 2 .
Claim 13
Dependent Claim
41 words
The method of claim 1 or 2 wherein R 1 is --NHCH 3 , --NHC 2 H 5 , --N(CH 3 ) 2 or --N(C 2 H 5 ) 2 and R 2 is --N(CH 2 CH 2 Cl) 2 .
Claim 14
Dependent Claim
26 words
The method of claim 1 or 2 wherein the treating step involves an incubation at about 37° C. for a period of 1 to 72 hours.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-10-07 | 1995-04-08 | 1995-10-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-10-07 | 1999-04-08 | 1999-10-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-10-07 | 2003-04-08 | 2003-10-08 | $7700.00 | Window Closed / Lapsed |