US5055415A
Expired / Lapsed
US
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Method for immunoassay procedure for the detection of antigen by reacting with antibody which increases its electrical charge and electrophoretic mobility
Assignee: Hitachi Ltd
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Filing: 1988-04-01
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Granted: 1991-10-08
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Expires: 2008-04-01
Abstract
Abstract A method for immunoassay for an antigen involves the following steps: reacting a sample possibly containing an antigen to be determined, a definite amount of a conjugate of the antigen and a charged substance and a definite amount of labeled antibody specific for antigen; allowing the reaction products to electrophoretically migrate towards a carrier bearing an attached antibody specific for the charged substance, so that when the reaction products contact the carrier any reaction product of labeled antibody and antigen passes through the carrier and any reaction product of the labeled antibody and conjugate binds to the carrier attached antibody specific for the charged substance; determining the amount of labeled antibody thereby bound to the carrier and relating the determined amount of labeled antibody to the amount of antigen in the sample.
Inventors
- 👤 Kyoko Imai
- 👤 Yasushi Nomura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 02:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/558; G01N33/561; G01N33/74; G01N33/743; G01N33/78; Y; Y10; Y10S; Y10S436/00; Y10S436/806; Y10S436/819
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
131 words
A method for immunoassay for an antigen comprising: reacting 1) a sample possibly containing an antigen to be determined, 2) a definite amount of a conjugate of the antigen and a charged substance, and 3) a definite amount of labeled antibody specific for antigen; allowing the reaction products to electrophoretically migrate towards a carrier bearing an attached antibody specific for the charged substance, whereby when said reaction products contact said carrier 1) any reaction product of labeled antibody and antigen passes through said carrier, and 2) any reaction product of labeled antibody and conjugate binds to the carrier attached antibody specific for the charged substance; determining the amount of labeled antibody thereby bound to the carrier; relating the determined amount of labeled antibody to the amount of antigen in the sample.
Claim 2
Independent Claim
131 words
A method for immunoassay for an antigen comprising: reacting 1) a sample possibly containing an antigen to be determined, 2) a definite amount of a conjugate of the antigen and a charged substance, and 3) a definite amount of labeled antibody specific for antigen; allowing the reaction products to electrophoretically migrate towards a carrier bearing an attached antibody specific for the charged substance, whereby when said reaction products contact said carrier 1) any reaction product of labeled antibody and antigen passes through said carrier, and 2) any reaction product of labeled antibody and conjugate binds to the carrier attached antibody specific for the charged substance; determining the amount of labeled antibody thereby bound to the carrier; relating the determined amount of labeled antibody to the amount of antigen in the sample.
Claim 3
Independent Claim
159 words
A method for immunoassay for an antigen comprising: reacting 1) a sample possibly containing an antigen to be determined, 2) a definite amount of a conjugate of the antigen and a charged substance, and 3) a definite amount of a non-labeled first antibody specific for the antigen; allowing the reaction products to electrophoretically migrate towards a carrier bearing an attached second antibody specific for the charged substance, whereby when said reaction products contact said carrier 1) any reaction product of first antibody and antigen passes through said carrier, and 2) any reaction product of first antibody and conjugate becomes bound to the carrier attached second antibody specific for the charged substance; reacting the first antibody thereby bound to the carrier with a labeled third antibody specific for the non-labeled first antibody; determining the amount of labeled third antibody thereby bound to the carrier; relating the determined amount of labeled third antibody to the amount of antigen in the sample.
Claim 4
Independent Claim
159 words
A method for immunoassay for an antigen comprising: reacting 1) a sample possibly containing an antigen to be determined, 2) a definite amount of a conjugate of the antigen and a charged substance, and 3) a definite amount of a non-labeled first antibody specific for the antigen; allowing the reaction products to electrophoretically migrate towards a carrier bearing an attached second antibody specific for the charged substance, whereby when said reaction products contact said carrier 1) any reaction product of first antibody and antigen passes through said carrier, and 2) any reaction product of first antibody and conjugate becomes bound to the carrier attached second antibody specific for the charged substance; reacting the first antibody thereby bound to the carrier with a labeled third antibody specific for the non-labeled first antibody; determining the amount of labeled third antibody thereby bound to the carrier; relating the determined amount of labeled third antibody to the amount of antigen in the sample.
Claim 5
Independent Claim
134 words
A method of immunoassay for an antigen comprising: reacting 1) a sample possibly containing an antigen to be determined, 2) a definite amount of a conjugate of the antigen and a labeled charged substance, and 3) a definite amount of a first antibody specific for antigen; allowing any reaction products or unreacted conjugate to electrophoretically migrate towards a carrier bearing an attached second antibody specific for the first antibody, whereby when said reaction products or unreacted conjugate contact said carrier 1) any unreacted conjugate passes through said carrier, and 2) any reaction product of first antibody with antigen or conjugate binds to the carrier attached second antibody; determining the amount of label in the conjugate thereby bound to the carrier; relating the determined amount of label to the amount of antigen in the sample.
Claim 6
Independent Claim
134 words
A method of immunoassay for an antigen comprising: reacting 1) a sample possibly containing an antigen to be determined, 2) a definite amount of a conjugate of the antigen and a labeled charged substance, and 3) a definite amount of a first antibody specific for antigen; allowing any reaction products or unreacted conjugate to electrophoretically migrate towards a carrier bearing an attached second antibody specific for the first antibody, whereby when said reaction products or unreacted conjugate contact said carrier 1) any unreacted conjugate passes through said carrier, and 2) any reaction product of first antibody with antigen or conjugate binds to the carrier attached second antibody; determining the amount of label in the conjugate thereby bound to the carrier; relating the determined amount of label to the amount of antigen in the sample.
Claim 7
Independent Claim
153 words
A method of immunoassay for an antigen comprising: reacting 1) a sample possibly containing an antigen to be determined, 2) a definite amount of a conjugate of the antigen and a charged substance, and 3) a definite amount of a first antibody specific for the antigen; allowing any reaction products or unreacted conjugate to electrophoretically migrate towards a carrier bearing an attached second antibody specific for the first antibody, whereby when said reaction products or unreacted conjugate contact said carrier 1) any unreacted conjugate passes through said carrier, and 2) any reaction product of first antibody with antigen or conjugate binds to the carrier attached second antibody; reacting the conjugate thereby bound to the carrier with a labeled third antibody specific for the charged substance; determining the amount of labeled third antibody thereby bound to the carrier; relating the determined amount of labeled third antibody to the amount of antigen in the sample.
Claim 8
Independent Claim
153 words
A method of immunoassay for an antigen comprising: reacting 1) a sample possibly containing an antigen to be determined, 2) a definite amount of a conjugate of the antigen and a charged substance, and 3) a definite amount of a first antibody specific for the antigen; allowing any reaction products or unreacted conjugate to electrophoretically migrate towards a carrier bearing an attached second antibody specific for the first antibody, whereby when said reaction products or unreacted conjugate contact said carrier 1) any unreacted conjugate passes through said carrier, and 2) any reaction product of first antibody with antigen or conjugate binds to the carrier attached second antibody; reacting the conjugate thereby bound to the carrier with a labeled third antibody specific for the charged substance; determining the amount of labeled third antibody thereby bound to the carrier; relating the determined amount of labeled third antibody to the amount of antigen in the sample.
Claim 9
Independent Claim
42 words
The method for immunossay according to any one of claimes 1 to 4 wherein the antigen to be determined is apparently electrically neutral or has no or slight electric chage so that the antigen cannot migrate or can slightly migrate by electrophoresis.
Claim 10
Independent Claim
23 words
The method for immunossay according to any one of claims 1 to 4 wherein the charged substance is a protein or a lipid.
Claim 11
Dependent Claim
23 words
The method for immunossay according to claim 6 wherein the protein is bovine serum albumin, rabbit serum albumin, human serum albumin, or globulin.
Claim 12
Dependent Claim
15 words
The method for immunossay according to claim 6 wherein the lipid is dicetyl phosphoric acid.
References Cited (Backward Citations)
US3654090A
Cited
US3654090B1
Cited
US3966897A
Cited
US4048298A
Cited
US4287300A
Cited
US4628035A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-10-07 | 1995-04-08 | 1995-10-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-10-07 | 1999-04-08 | 1999-10-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-10-07 | 2003-04-08 | 2003-10-08 | $7700.00 | Window Closed / Lapsed |