US5045535A
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US
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Method, apparatus and a composition for a polysaccharide-containing matrix having covalently bound haptens
Assignee: Roche Diagnostics GmbH
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Filing: 1988-12-09
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Granted: 1991-09-03
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Expires: 2008-12-09
Abstract
Abstract The present invention provides a process for the production of a polysaccharide matrix to which haptens are covalently bound, wherein polysaccharide-containing material is rendered alkaline, dried to a water content of less than 5% and then reacted in an anhydrous medium with a hapten containing at least one activated functional group. The present invention also provides a device for carrying out this process, which comprises a winding core (3) in a container (1) for a reaction solution which has a tube (11), provided on its wall with a plurality of openings (7) and closed on one end (9), for the reception of a winding (13) of the material to be treated and two axial end walls (15, 17) for sealing off the axial ends of the winding (13) and feeds the reaction solution from the container (1) into the other end (21) of the tube (11) by means of a circulating pump cycle (19).
Inventors
- 👤 Thomas Mang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 16:26
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/281; B01J20/282; B01J20/285; B01J20/22; B01J20/24; B01J20/26; B01J20/286; B01J20/30; B01J20/3085; B01J20/32; B01J20/3202; B01J20/3206; B01J20/3208; B01J20/3212; B01J20/3214; B01J20/3217; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3251; B01J20/3253; B01J20/3255; B01J20/3291; B01J20/3293; C; C08; C08B; C08B15/00; C08B17/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/548; B01J2220/00; B01J2220/40; B01J2220/48; B01J2220/4812; B01J2220/4825; B01J2220/50; B01J2220/54; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
138 words
A method for the production of a polysaccharide matrix to which a hapten is covalently bound for use in affinity chromatography or immunoassay comprising rendering a polysaccharide-containing material of the matrix alkaline, drying the polysaccharide-containing material of the matrix to a water content of less than 5%, reacting the dried polysaccharide-containing material of the matrix with an activated hapten comprising at least one activated carboxyl, amino or hydroxyl functional group whereby the hapten is bound to the polysaccharide in an anhydrous organic solvent in a range of polysaccharide:hapten of 10 4 to 0.1:1 to form a polysaccharide matrix so that the hapten retains its activity and is so bound to the matrix that it is not removed in solution or during reaction, and washing the reacted matrix with an organic solvent and an aqueous buffer of pH 6-8.
Claim 2
Independent Claim
138 words
A method for the production of a polysaccharide matrix to which a hapten is covalently bound for use in affinity chromatography or immunoassay comprising rendering a polysaccharide-containing material of the matrix alkaline, drying the polysaccharide-containing material of the matrix to a water content of less than 5%, reacting the dried polysaccharide-containing material of the matrix with an activated hapten comprising at least one activated carboxyl, amino or hydroxyl functional group whereby the hapten is bound to the polysaccharide in an anhydrous organic solvent in a range of polysaccharide:hapten of 10 4 to 0.1:1 to form a polysaccharide matrix so that the hapten retains its activity and is so bound to the matrix that it is not removed in solution or during reaction, and washing the reacted matrix with an organic solvent and an aqueous buffer of pH 6-8.
Claim 3
Dependent Claim
18 words
The method of claim 1 wherein the polysaccharide-containing material which is rendered alkaline is a cellulose containing material.
Claim 4
Dependent Claim
25 words
The method of claim 2 wherein the cellulose containing material rendered alkaline is cellulose, sulphite wood pulp, viscose stable, cellulose acetate or a mixture thereof.
Claim 5
Dependent Claim
21 words
The method of claim 1 further comprising rendering the polysaccharide-containing material alkaline by treatment with aqueous 0.005-5 N alkali metal hydroxide.
Claim 6
Dependent Claim
19 words
The method of claim 1 further comprising rendering the polysaccharide-containing material alkaline by treatment with an alkali metal alcoholate.
Claim 7
Dependent Claim
22 words
The method of claim 1 further comprising drying the polysaccharide-containing material of the matrix to a water content of less than 2%.
Claim 8
Dependent Claim
44 words
The method of claim 1 further comprising reacting the polysaccharide-containing material of the matrix with a hapten comprising an activated carboxyl group wherein the activation of the carboxyl group takes place with an acid chloride, a hydroxyimide, a carbodiimide, an anhydride or an imidazolide.
Claim 9
Dependent Claim
38 words
The method of claim 1 further comprising reacting the polysaccharide-containing material of the matrix with an activated hapten selected from the group consisting of T 3 , T 4 , digoxin, diphenylhydrantoin, folate, biotin and a derivative thereof.
Claim 10
Dependent Claim
58 words
A polysaccharide matrix composition comprising a polysaccharide-containing material with a water content of less than 5% and a hapten bound covalently thereto via an activated carboxyl, hydroxyl or amino group on the hapten and wherein the hapten retains its activity and is so bound to the matrix that it is not removed while in solution or during reaction.
Claim 11
Dependent Claim
58 words
A polysaccharide matrix composition comprising a polysaccharide-containing material with a water content of less than 5% and a hapten bound covalently thereto via an activated carboxyl, hydroxyl or amino group on the hapten and wherein the hapten retains its activity and is so bound to the matrix that it is not removed while in solution or during reaction.
Claim 12
Dependent Claim
29 words
The polysaccharide matrix of claim 9 wherein the polysaccharide-containing material is selected from the group consisting of cellulose, sulphite wood pulp, viscose staple, celulose acetate and a mixture thereof.
Claim 13
Dependent Claim
18 words
The polysaccharide matrix of claim 9 comprising a polysaccharide-containing material with a water content of less than 2%.
Claim 14
Dependent Claim
29 words
The composition of claim 9 wherein the hapten is selected from the group consisting of T 3 , T 4 , digoxin, diphenylhydantoin, folate, biotin and a derivative thereof.
References Cited (Backward Citations)
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