US5037378A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for coating a flexible tubular prosthesis with living cells
Assignee: Sulzer AG
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Filing: 1989-09-25
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Granted: 1991-08-06
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Expires: 2009-09-25
Abstract
Abstract The coating process for coating the vascular prosthesis resides in stepwise rotations of the prosthesis, filled with a cell suspension, through fixed angles and in pauses for deposition between the rotations. The length of pauses start at two seconds and are increased in small increments of from 1 to 2 seconds to values of 2 to 3 minutes. The angle of rotation is controlled to ensure a uniform gapless deposition of the living cells.
Inventors
- 👤 Werner Muller
- 👤 Marie-Claude Hensel
- 👤 August Huber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:09
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/38; A61L27/3895; A61F; A61F2/00; A61F2/02; A61F2/04; A61F2/06; A61F2/062; A61L27/50; A61L27/507; Y; Y10; Y10S; Y10S623/00; Y10S623/92; Y10S623/921
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
164 words
A process for coating a flexible tubular vascular plastic prosthesis with living cells, said process comprising the steps of seeding the interior of the tubular prosthesis with a cooled cell suspension at a temperature sufficient to preclude precipitation of cells from the suspension; heating the suspension-filled prosthesis to approximately body temperature while rotating the prosthesis about a horizontally disposed longitudinal axis thereof to prevent premature uncontrolled deposition of cells; interrupting rotation of the prosthesis for a short initial pause of at most two seconds to permit sedimentation of cells onto the interior surface of the prosthesis; thereafter sequentially rotating the prosthesis over a predetermined angle and interrupting rotation of the prosthesis for predetermined pauses of time over a predetermined time to permit deposition of cells from the suspension in at least one uniform layer on the interior surface of the prosthesis without the occurrence of a peripheral striation; and thereafter continuously rotating the prosthesis while extracting the suspension with the remaining unprecipitated cells therein.
Claim 2
Dependent Claim
164 words
A process for coating a flexible tubular vascular plastic prosthesis with living cells, said process comprising the steps of seeding the interior of the tubular prosthesis with a cooled cell suspension at a temperature sufficient to preclude precipitation of cells from the suspension; heating the suspension-filled prosthesis to approximately body temperature while rotating the prosthesis about a horizontally disposed longitudinal axis thereof to prevent premature uncontrolled deposition of cells; interrupting rotation of the prosthesis for a short initial pause of at most two seconds to permit sedimentation of cells onto the interior surface of the prosthesis; thereafter sequentially rotating the prosthesis over a predetermined angle and interrupting rotation of the prosthesis for predetermined pauses of time over a predetermined time to permit deposition of cells from the suspension in at least one uniform layer on the interior surface of the prosthesis without the occurrence of a peripheral striation; and thereafter continuously rotating the prosthesis while extracting the suspension with the remaining unprecipitated cells therein.
Claim 3
Dependent Claim
16 words
A process as set forth in claim 1 wherein said predetermined angle is other than π.
Claim 4
Dependent Claim
22 words
A process as set forth in claim 1 wherein said predetermined angle is an unremaindered fraction of π without a common divisor.
Claim 5
Dependent Claim
17 words
A process as set forth in claim 1 wherein said predetermined time is of several hours duration.
Claim 6
Dependent Claim
24 words
A process as set forth in claim 1 wherein said predetermined pauses of time are of increasing durations of from 1 to 2 seconds.
Claim 7
Dependent Claim
31 words
A process as set forth in claim 1 which further comprises the step of saturating the prosthesis with an aqueous physiological solution to effect an equalization treatment prior to seeding thereof.
Claim 8
Dependent Claim
21 words
A process as set forth in claim 6 wherein the solution is one of a salt solution and a nutrient solution.
Claim 9
Dependent Claim
15 words
A process as set forth in claim 1 wherein the deposited cells are epithelial cells.
Claim 10
Dependent Claim
16 words
A process as set forth in claim 1 wherein the deposited cells are of different types.
Claim 11
Dependent Claim
21 words
A process as set forth in claim 9 wherein the cells of different type form at least two of said layers.
Claim 12
Dependent Claim
23 words
A process as set forth in claim 1 wherein the angle of rotation of the prosthesis increased between pauses in multiples of 2π.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-08-05 | 1995-02-04 | 1995-08-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-08-05 | 1999-02-04 | 1999-08-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-08-05 | 2003-02-04 | 2003-08-06 | $7700.00 | Window Closed / Lapsed |