Continuous concentration monitoring by circular dichroism

Claim 1

An apparatus for continuously monitoring the concentration of a subject compound in a flowing solution, comprising: means for generating a beam of alternately left circularly polarized and right circularly polarized monochromatic electromagnetic radiation directed along a path; a flow cell positioned down said path from the means for generating and having first and second separate straight channels therethrough which intersect at a single point, the first channel being arranged so the flowing solution passed therethrough and the second channel being arranged so that the beam of electromagnetic radiation passes therethrough; first and second transparent plates, comprised of a material which has no adverse dichroic effects on the beam of electromagnetic radiation, in said flow cell positioned relative to each other and to said channels so that said beam of electromagnetic radiation traverses said first plate, then said flowing solution, and then said second transparent plate before exiting the flow cell, the first and second transparent plates being located to isolate said first channel from said second channel; means for continuously detecting the intensity of said beam of electromagnetic radiation after passage through the flowing solution in said flow cell and converting the intensity measure into an electronic signal comprising a direct current component and an alternating current component; means for continuously determining the concentration of the subject compound in the flowing solution by measuring a ratio of the direct current component to the alternating current component to the alternating current component.

Claim 2

An apparatus for continuously monitoring the concentration of a subject compound in a flowing solution, comprising: means for generating a beam of alternately left circularly polarized and right circularly polarized monochromatic electromagnetic radiation directed along a path; a flow cell positioned down said path from the means for generating and having first and second separate straight channels therethrough which intersect at a single point, the first channel being arranged so the flowing solution passed therethrough and the second channel being arranged so that the beam of electromagnetic radiation passes therethrough; first and second transparent plates, comprised of a material which has no adverse dichroic effects on the beam of electromagnetic radiation, in said flow cell positioned relative to each other and to said channels so that said beam of electromagnetic radiation traverses said first plate, then said flowing solution, and then said second transparent plate before exiting the flow cell, the first and second transparent plates being located to isolate said first channel from said second channel; means for continuously detecting the intensity of said beam of electromagnetic radiation after passage through the flowing solution in said flow cell and converting the intensity measure into an electronic signal comprising a direct current component and an alternating current component; means for continuously determining the concentration of the subject compound in the flowing solution by measuring a ratio of the direct current component to the alternating current component to the alternating current component.

Claim 9

A method for continuously measuring the concentration of a subject compound in a flowing solution, which compound exhibits circular dichroism activity, comprising: providing the flowing solution which includes the subject compound; directing the flowing solution through a flow cell; generating a beam of electromagnetic radiation with a wavelength corresponding to the maximum absorbance by the subject compound which is alternately left circularly polarized and right circularly polarized and which is directed along a path; passing the beam of electromagnetic radiation through said flowing solution in said flow cell; continuously detecting the intensity of the beam of electromagnetic radiation after passage through the flowing solution in said flow cell and converting the intensity into an electronic signal comprising a direct current component and an alternating current component, the alternating current component representing the difference of absorption of the left circularly polarized beam and the right circularly polarized beam; continuously determining the concentration of the subject compound in the flowing solution by measuring the ratio of the alternating current to the direct current component.

Claim 10

A method for continuously measuring the concentration of a subject compound in a flowing solution, which compound exhibits circular dichroism activity, comprising: providing the flowing solution which includes the subject compound; directing the flowing solution through a flow cell; generating a beam of electromagnetic radiation with a wavelength corresponding to the maximum absorbance by the subject compound which is alternately left circularly polarized and right circularly polarized and which is directed along a path; passing the beam of electromagnetic radiation through said flowing solution in said flow cell; continuously detecting the intensity of the beam of electromagnetic radiation after passage through the flowing solution in said flow cell and converting the intensity into an electronic signal comprising a direct current component and an alternating current component, the alternating current component representing the difference of absorption of the left circularly polarized beam and the right circularly polarized beam; continuously determining the concentration of the subject compound in the flowing solution by measuring the ratio of the alternating current to the direct current component.