US5032511A
Expired / Lapsed
US
⏱️ 0.0 years remaining
DNA fragments coding for antigens specific to non-A non-B hepatitis, expression vectors containing said DNA fragments, transformants and process for producing said antigens
Assignee: Mitsubishi Chemical Corp
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Filing: 1988-03-15
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Granted: 1991-07-16
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Expires: 2008-03-15
Abstract
Abstract Provided herein are a DNA fragment which contains a base sequence coding for a non-A non-B hepatitis-specific antigenic protein occurring in cells of the liver affected with non-A non-B hepatitis, an expression vector in which said DNA fragment is inserted into a cloning site present downstream from a promoter thereof, a transformant obtained by introducing said expression vector into a host, and a process for producing said antigenic protein which comprises providing said expression vector, transforming a host with said expression vector, culturing the transforming host and collecting the protein produced therein.
Inventors
- 👤 Kazuhiro Takahashi
- 👤 Tatsurou Shibui
- 👤 Michiru Kamizono
- 👤 Rie Matsui
- 👤 Yutaka Teranishi
- 👤 Shigetada Nakanishi
- 👤 Naomi Kitamura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 20:23
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07K; C07K14/00; C07K14/005; C12N2770/00; C12N2770/00011; C12N2770/24011; C12N2770/24211; C12N2770/24222
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
39 words
An isolated DNA fragment that contains a base sequence coding for a non-A non-B hepatitis-specific antigenic protein which occurs in cells of liver affected with non-A non-B hepatitis, which protein is represented by the following amino acid sequence: ##STR4##
Claim 2
Dependent Claim
39 words
An isolated DNA fragment that contains a base sequence coding for a non-A non-B hepatitis-specific antigenic protein which occurs in cells of liver affected with non-A non-B hepatitis, which protein is represented by the following amino acid sequence: ##STR4##
Claim 3
Dependent Claim
23 words
The DNA fragment in accordance with claim 1, in which the cells of the liver are derived from a human or chimpanzee individual.
Claim 4
Dependent Claim
19 words
The DNA fragment in accordance with claim 1, in which the base sequence comprises the following base sequence: ##STR5##
Claim 5
Dependent Claim
47 words
An expression vector comprising a promoter, a ribosome binding sequence and a base sequence coding for a non-A non-B hepatitis-specific antigen as recited in claim 1 at a cloning site present downstream from the promoter such that transcription of said base sequence is initiated by said promoter.
Claim 6
Dependent Claim
21 words
The expression vector in accordance with claim 4 additionally comprising a repressor gene which codes for a repressor of said promoter.
Claim 7
Dependent Claim
16 words
The expression vector in accordance with claim 4, in which the promoter operates in a microorganism.
Claim 8
Dependent Claim
16 words
The expression vector in accordance with claim 4, in which the promoter operates in an eukaryote.
Claim 9
Dependent Claim
58 words
A transformant obtained by transforming a host with an expression vector, said expression vector comprising a promoter, a ribosome binding sequence, and a base sequence coding for a non-A non-B hepatitis-specific antigen as recited in claim 1 at a cloning site present downstream from said promoter such that transcription of said base sequence is affected by said promoter.
Claim 10
Dependent Claim
17 words
The transformant in accordance with claim 8, in which the host is Escherichia coli or Bacillus subtilis.
Claim 11
Dependent Claim
82 words
A process for producing an antigen specific to non-A non-B hepatitis, comprising the steps of: introducing a promoter, a ribosome binding sequence, and a base sequence coding for an antigen as recited in claim 1 into an expression vector, said base sequence being downstream from said promoter, such that transcription of said base sequence is initiated by said promoter, introducing the expression vector containing said DNA fragment into a host, culturing said transformed host, and collecting the antigen produced by said host.
Claim 12
Dependent Claim
18 words
An expression vector as claimed in claim 4, additionally comprising a transcription terminator downstream of said base sequence.
Claim 13
Dependent Claim
21 words
A process as claimed in claim 4, additionally comprising the step of introducing a transcription terminator downstream of said base sequence.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-07-15 | 1995-01-14 | 1995-07-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-07-15 | 1999-01-14 | 1999-07-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-07-15 | 2003-01-14 | 2003-07-16 | $7700.00 | Window Closed / Lapsed |