US5030697A
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Polymer-bound dyes, process for their production and use
Assignee: Bayer AG
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Filing: 1989-09-18
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Granted: 1991-07-09
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Expires: 2009-09-18
Abstract
Abstract A polymer-bound linkable dye comprising a) a water-soluble polymer backbone, b) a dye covalently bound thereto, and c) functional groups which enable the polymer-dye to link covalently with biological materials, in which the water-soluble polymer backbone is a copolymer which contains arcylamide, methacrylamide, N-C 1 -C 4 alkyl(meth)acrylamide, N,N-C 1 -C 4 dialkylacrylamide, N-vinylpyrrolidone, N-vinylpiperidone, N-vinylcaprolactam, N-vinylformamide, N-vinylacetamide, N-vinyl-N-methylacetamide, N-vinyl-O-methylurethane, ethene or vinylmethylether as nonionic monomer blocks. The polymer-bound dye can be linked to a biologically active material such as an antibody or nucleic acid and used analytically.
Inventors
- 👤 Herbert Hugl
- 👤 Bruno Bomer
- 👤 Heinz Kolbl
- 👤 Florin Seng
- 👤 Eberhard Kuckert
- 👤 Gunter Sackmann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 14:57
CPC Classifications
C; C09; C09B; C09B69/00; C09B69/10; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
132 words
A polymer-bond linkable dye composition of the formula ##STR20## wherein A are monomers which can be copolymerized with unsaturated dicarboxylic anhydrides, B are radicals of maleic acid, itaconic acid or citraconic acid without any carboxylic groups, F are radicals of a dyestuff containing one or more primary and/or secondary amino groups, Y are reactive groups for antigens and DNA with a reactive functional end group, X are groups which increase the solubility in water of the polymer dyestuff, R are C 2 - to C 12 -alkylene, cycloalkylene and/or arylene groups, a is between 3 to 10 3 and smaller than n, b is between 1 and 5, c is zero or between 1 and 100, m is between 25 and 10 4 , n is between 3 and 10 3 .
Claim 2
Dependent Claim
132 words
A polymer-bond linkable dye composition of the formula ##STR20## wherein A are monomers which can be copolymerized with unsaturated dicarboxylic anhydrides, B are radicals of maleic acid, itaconic acid or citraconic acid without any carboxylic groups, F are radicals of a dyestuff containing one or more primary and/or secondary amino groups, Y are reactive groups for antigens and DNA with a reactive functional end group, X are groups which increase the solubility in water of the polymer dyestuff, R are C 2 - to C 12 -alkylene, cycloalkylene and/or arylene groups, a is between 3 to 10 3 and smaller than n, b is between 1 and 5, c is zero or between 1 and 100, m is between 25 and 10 4 , n is between 3 and 10 3 .
Claim 3
Dependent Claim
29 words
A polymer-bound linkable fluorescent dye according to claim 1, wherein the dye molecule is selected from the group consisting of a coumarin, carbostyril, pyrazoline, naphthalimide, pyrene, fluorescein or rhodanine.
Claim 4
Dependent Claim
26 words
A polymer-bound linkable dye according to claim 1, wherein the dye is selected from the group consisting of an azo dye, methine dye or anthraquinone dye.
Claim 5
Dependent Claim
26 words
A polymer-bound linkable dye according to claim 1, containing a functional group selected from the group consisting of a hydroxyl, amino, carboxyl, thio and sothiocyanate group.
Claim 6
Dependent Claim
47 words
A polymer-bound linkable dye according to claim 1, in which a) the water-soluble polymer backbone is an acrylamidemaleic anhydride copolymer, b) the dye is selected from the group consisting of a coumarin, fluorescein and rhodamine, and c) the functional groups are introduced by reaction with an aminoalcohol.
Claim 7
Dependent Claim
49 words
A polymer-bound linkable dye according to claim 1, in which a) the water-soluble polymer backbone is a vinyl pyrrolidone maleic anhydride copolymer, b) the dye is selected from the group consisting of a coumarin, fluorescein and rhodamine, and c) the functional groups are introduced by reaction with an aminoalcohol.
References Cited (Backward Citations)
US4166105A
Cited
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