US5017486A
Expired / Lapsed
US
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Cloning of DNA encoding antibacterial polypeptide precursor
Assignee: Sanwa Kagaku Kenkyusho Co Ltd
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Filing: 1988-07-25
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Granted: 1991-05-21
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Expires: 2008-07-25
Abstract
Abstract A cloned single-strand DNA comprising nucleotide sequence which encodes an antibacterial polypeptide precursor, a cloned double-strand DNA consisting of the single-strand DNA and its complementary single-strand DNA, a DNA fragment of the single- or double-strand DNA, a process for the preparation thereof, and a plasmid, in which the double-strand DNA or its fragment is inserted are disclosed.
Inventors
- 👤 Kiichi Sawai
- 👤 Shunji Natori
- 👤 Haruo Takahashi
- 👤 Kenichi Tanaka
- 👤 Takahiko Mitani
- 👤 Masayasu Kurono
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 14:04
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/43504; C07K14/43563; C07K14/43577; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
21 words
A recombinant DNA molecule comprising about 190 nucleotides which encodes an antibacterial polypeptide precursor, having the following amino acid sequence Met-Asn-Phe-Asn-Lys-Val-Phe-Val-Phe-Phe-Ala-Val-Leu-Met-Val-Ala-Phe-Met-Gly-Gln-Ser-Glu-Ala-Gly-Trp-Leu-Lys-Lys-lle-Gly-Lys-Lys-lle-Glu-Arg-Val-Gly-Gln-His-Thr-Arg-Asp-Ala-Thr-lle-Gln-Thr-lle-Ala-Val-Ala-Gln-Gln-Ala-Ala-Asn-Val-Ala-Ala-Thr-Leu-Lys-Gly.
Claim 2
Dependent Claim
21 words
A recombinant DNA molecule comprising about 190 nucleotides which encodes an antibacterial polypeptide precursor, having the following amino acid sequence Met-Asn-Phe-Asn-Lys-Val-Phe-Val-Phe-Phe-Ala-Val-Leu-Met-Val-Ala-Phe-Met-Gly-Gln-Ser-Glu-Ala-Gly-Trp-Leu-Lys-Lys-lle-Gly-Lys-Lys-lle-Glu-Arg-Val-Gly-Gln-His-Thr-Arg-Asp-Ala-Thr-lle-Gln-Thr-lle-Ala-Val-Ala-Gln-Gln-Ala-Ala-Asn-Val-Ala-Ala-Thr-Leu-Lys-Gly.
Claim 3
Dependent Claim
105 words
A recombinant DNA molecule as claimed in claim 1, wherein said DNA has a nucleotide sequence of __________________________________________________________________________
5'---
ATG AAT TTC AAT AAA GTT TTT GTA TTT TTT GCT GTA CTT ATG GTC GCT
TTT ATG GGT CAA AGT GAA GCT GGT TGG TTA AAA AAG ATT GGC AAG AAA
ATT GAA CGT GTT GGT CAA CAT ACC CGT GAT GCC ACT ATA CAG ACA ATA
GCT GTA GCC CAG CAG GCA GCA AAT GTT GCT GCT ACT TTA AAG
__________________________________________________________________________
GGT---3' wherein A, C, G and T are, respectively, a deoxyribonucleotide having adenine, cytosine, guanine or thymine base and all sequences degenerate therewith.
Claim 4
Dependent Claim
20 words
A recombinant DNA fragment, wherein said DNA consists essentially of a nucleotide sequence encoding the following amino acid sequence Met-Asn-Phe-Asn-Lys-Val-Phe-Val-Phe-Phe-Ala-Val-Leu-Met-Val-Ala-Phe-Met-Gly-Gln-Ser-Glu-Ala-Gly-Trp-Leu-Lys-Lys-lle-Gly-Lys-Lys-lle-Glu-Arg-Val-Gly-Gln-His-Thr-Arg-Asp-Ala-Thr-lle-Gln-Thr-lle-Ala-Val-Ala-Gln-Gln-Ala-Ala-Asn-Val-Ala-Ala-Thr-Leu-Lys-Gly.
Claim 5
Dependent Claim
20 words
A recombinant DNA fragment, wherein said DNA consists essentially of a nucleotide sequence encoding the following amino acid sequence Met-Asn-Phe-Asn-Lys-Val-Phe-Val-Phe-Phe-Ala-Val-Leu-Met-Val-Ala-Phe-Met-Gly-Gln-Ser-Glu-Ala-Gly-Trp-Leu-Lys-Lys-lle-Gly-Lys-Lys-lle-Glu-Arg-Val-Gly-Gln-His-Thr-Arg-Asp-Ala-Thr-lle-Gln-Thr-lle-Ala-Val-Ala-Gln-Gln-Ala-Ala-Asn-Val-Ala-Ala-Thr-Leu-Lys-Gly.
Claim 6
Dependent Claim
19 words
A plasmid comprising about 190 nucleotides which encodes an antibacterial polypeptide precursor having the following amino acid sequence Met-Asn-Phe-Asn-Lys-Val-Phe-Val-Phe-Phe-Ala-Val-Leu-Met-Val-Ala-Phe-Met-Gly-Gln-Ser-Glu-Ala-Gly-Trp-Leu-Lys-Lys-lle-Gly-Lys-Lys-lle-Glu-Arg-Val-Gly-Gln-His-Thr-Arg-Asp-Ala-Thr-lle-Gln-Thr-lle-Ala-Val-Ala-Gln-Gln-Ala-Ala-Asn-Val-Ala-Ala-Thr-Leu-Lys-Gly.
Claim 7
Dependent Claim
19 words
A plasmid comprising about 190 nucleotides which encodes an antibacterial polypeptide precursor having the following amino acid sequence Met-Asn-Phe-Asn-Lys-Val-Phe-Val-Phe-Phe-Ala-Val-Leu-Met-Val-Ala-Phe-Met-Gly-Gln-Ser-Glu-Ala-Gly-Trp-Leu-Lys-Lys-lle-Gly-Lys-Lys-lle-Glu-Arg-Val-Gly-Gln-His-Thr-Arg-Asp-Ala-Thr-lle-Gln-Thr-lle-Ala-Val-Ala-Gln-Gln-Ala-Ala-Asn-Val-Ala-Ala-Thr-Leu-Lys-Gly.
Claim 8
Dependent Claim
87 words
A process for the preparation of a recombinant DNA molecule comprising about 190 nucleotides which encodes an antibacterial polypeptide precursor, said process comprising isolating poly(A)RNA from a cell line established from Sarcophaga peregrina embryo, constructing a cDNA library with the poly(A)RNA and vector/primer DNA transforming Esherichia coli, screening said transformed Esherichia coli by hybridization using a probe prepared by cleaving a cDNA clone of a known antibacterial polypeptide obtained from a larva of Sarcophaga peregrina with restriction enzymes of PstII and AflIII and labeling the resulting fragment.
Claim 9
Dependent Claim
87 words
A process for the preparation of a recombinant DNA molecule comprising about 190 nucleotides which encodes an antibacterial polypeptide precursor, said process comprising isolating poly(A)RNA from a cell line established from Sarcophaga peregrina embryo, constructing a cDNA library with the poly(A)RNA and vector/primer DNA transforming Esherichia coli, screening said transformed Esherichia coli by hybridization using a probe prepared by cleaving a cDNA clone of a known antibacterial polypeptide obtained from a larva of Sarcophaga peregrina with restriction enzymes of PstII and AflIII and labeling the resulting fragment.
References Cited (Backward Citations)
JPS5913730A
Cited
JPS61122299A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-05-20 | 1994-11-19 | 1995-05-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-05-20 | 1998-11-19 | 1999-05-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-05-20 | 2002-11-19 | 2003-05-21 | $7700.00 | Window Closed / Lapsed |