US5017375A
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US
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Method to prepare a neurotrophic composition
Assignee: Baylor College of Medicine
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Filing: 1987-05-18
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Granted: 1991-05-21
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Expires: 2007-05-18
Abstract
Abstract The present invention is based on the discovery that amyotrophic lateral sclerosis (ALS), Parkinson disease and Alzheimer disease are due to lack of a disorder-specific neurotrophic hormone or factor. Diagnosis is accomplished by assaying factors specific for a particular neuronal network or system; for example, dopamine neutotrophic hormones from striatum or caudate-putamen in the nigrostriatal dopaminergic neural system are used to diagnose and treat parkinsonism. With tissue culture, the presence or absence of spacific neurotrophic factos can be assessed in ALS, parkinsonism, and Alzheimer disease. If there is a deficiency, extracted and purified neurotrophic factors specific to the particular neuronal network or system can be injected into a patient having ALS, Alzheimer disease or parkinsonism for treatment of the disease.
Inventors
- 👤 Stanley H. Appel
- 👤 Yasuko Tomozawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 07:18
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/839
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
55 words
A method to prepare a neurotrophic composition, which method comprises: (a) extracting factors from caudate-putamen tissue of a normal mammal; (b) assaying the caudate-putamen extract for trophic effects on dopaminergic neurons obtained from substantia nigra as measured by the ability to stimulate dopamine uptake by said neurons; and (c) isolating factors having said trophic effects.
Claim 2
Independent Claim
55 words
A method to prepare a neurotrophic composition, which method comprises: (a) extracting factors from caudate-putamen tissue of a normal mammal; (b) assaying the caudate-putamen extract for trophic effects on dopaminergic neurons obtained from substantia nigra as measured by the ability to stimulate dopamine uptake by said neurons; and (c) isolating factors having said trophic effects.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein said normal mammal is of bovine source.
Claim 4
Dependent Claim
23 words
The method of claim 1 wherein said extraction comprises homogenizing the tissue extract in aqueous solution, centrifuging the homogenate, and recovering the supernatant.
Claim 5
Dependent Claim
14 words
The method of claim 1 which further comprises purifying the isolated factors by fractionation.
Claim 6
Dependent Claim
19 words
The method of claim 4 wherein said purification yields dopaminergic neurotrophic factors having molecular weights less than 3,000 daltons.
Claim 7
Dependent Claim
22 words
The method of claim 5 wherein a dopaminergic neurotrophic factor has a molecular weight range of about 1,000 to about 1,600 daltons.
Claim 8
Dependent Claim
22 words
The method of claim 5 wherein a dopaminergic neurotrophic factor has a molecular weight range of about 1,500 to about 1,800 daltons.
Claim 9
Dependent Claim
17 words
The method of claim 5 which further comprises purifying the dopaminergic neurotrophic factor by cation-exchange HPLC chromatography.
Claim 10
Dependent Claim
28 words
The method of claim 3 wherein said tissue extract is prepared from fresh tissue and said aqueous solution comprises a buffer supplemented with one or more protease inhibitors.
Claim 11
Dependent Claim
16 words
The method of claim 9 which further comprises purifying said isolated factors by gel filtration chromatography.
Claim 12
Dependent Claim
19 words
The method of claim 10 wherein said purification yields dopaminergic neurotrophic factors having molecular weights less than 3,000 daltons.
Claim 13
Dependent Claim
22 words
The method of claim 5 wherein a dopaminergic neurotrophic factor has a molecular weight range of about 1,800 to about 2,600 daltons.
Claim 14
Dependent Claim
22 words
The method of claim 11 wherein a dopaminergic neurotrophic factor has a molecular weight range of about 1,500 to about 1,800 daltons.
Claim 15
Dependent Claim
22 words
The method of claim 11 wherein a dopaminergic neurotrophic factor has a molecular weight range of about 1,000 to about 1,600 daltons.
Claim 16
Dependent Claim
19 words
The method of claim 11 wherein said purification further comprises purifying the dopaminergic neurotrophic factors by anion-exchange HPLC chromatography.
Claim 17
Dependent Claim
9 words
A factor prepared by the method of claim 7.
Claim 18
Dependent Claim
9 words
A factor prepared by the method of claim 12.
References Cited (Backward Citations)
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| 3.5 Years (Year 4) | 1994-05-20 | 1994-11-19 | 1995-05-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-05-20 | 1998-11-19 | 1999-05-21 | $3760.00 | Window Closed / Lapsed |
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