US5013660A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of implanting living cells with a foreign substance
Assignee: National Institute of Advanced Industrial Science and Technology
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Filing: 1990-09-05
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Granted: 1991-05-07
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Expires: 2010-09-05
Abstract
Abstract Disclosed is a method and apparatus implanting living cells with a foreign substance with the aid of laser beams. According to this invention living cells are exposed to a laser microbeam of energy sufficient to modify a selected part of the cell to be temporarily transparent to a foreign substance. After the foreign substance gets in the host cell, the selected part of the cell recovers to the original state, thereby confining the foreign substance in the cell.
Inventors
- 👤 Takahiro Kasuya
- 👤 Yoji Ikawa
- 👤 Motowo Tsukakoshi
- 👤 Shunichi Kurata
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 18:40
CPC Classifications
H; H01; H01S; H01S3/00; H01S3/0007; C; C12; C12M; C12M35/00; C12M35/02; C12N; C12N1/00; C12N1/005; C12N13/00; C12N15/00; C12N15/09; C12N15/87
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
80 words
Method of introducing a foreign substance into living eukaryotic cells comprising exposing a suspension of living eukaryotic cells in a liquid medium to a laser beam whereby at least some of said living eukaryotic cells become temporarily permeable to said foreign substance, and adding the foreign substance to the liquid medium before or after said exposure, whereby said temporarily permeable eukaryotic cells are associated with said foreign substance so that said foreign substance can enter said temporarily permeable eukaryotic cells.
Claim 2
Dependent Claim
80 words
Method of introducing a foreign substance into living eukaryotic cells comprising exposing a suspension of living eukaryotic cells in a liquid medium to a laser beam whereby at least some of said living eukaryotic cells become temporarily permeable to said foreign substance, and adding the foreign substance to the liquid medium before or after said exposure, whereby said temporarily permeable eukaryotic cells are associated with said foreign substance so that said foreign substance can enter said temporarily permeable eukaryotic cells.
Claim 3
Dependent Claim
14 words
Method according to claim 1 wherein said laser beam is a continuous wave laser.
Claim 4
Dependent Claim
21 words
Method according to claim 2 wherein said living eukaryotic cells are transported through the path of said continuous wave laser beam.
Claim 5
Dependent Claim
43 words
Method according to claim 2 wherein the living eukaryotic cells are exposed to the laser beam by sweeping said continuous wave laser beam across a predetermined area of the suspension after said living eukaryotic cells are brought into association with said foreign substance.
Claim 6
Dependent Claim
21 words
Method according to claim 2 wherein said liquid medium is a medium appropriate for the cultivation of said living eukaryotic cells.
Claim 7
Dependent Claim
14 words
Method according to claim 1, wherein said laser beam is a pulsed laser beam.
Claim 8
Dependent Claim
12 words
Method according to claim 1 wherein said foreign substance is genetic material.
Claim 9
Dependent Claim
12 words
Method according to claim 1 wherein the living cells are animal cells.
Claim 10
Dependent Claim
12 words
Method according to claim 1 wherein the living cells are plant cells.
References Cited (Backward Citations)
FR2224902A1
Cited
US4071657A
Cited
US4302670A
Cited
US4338397A
Cited
WO1982004443A1
Cited
JPS5876091A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-05-06 | 1994-11-05 | 1995-05-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-05-06 | 1998-11-05 | 1999-05-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-05-06 | 2002-11-05 | 2003-05-07 | $7700.00 | Window Closed / Lapsed |