US5002875A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Plasimids containing the gene for DNA polymerase I from Streptococcus pneumoniae
Assignee: United States Department of Energy
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Filing: 1987-08-28
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Granted: 1991-03-26
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Expires: 2007-08-28
Abstract
Abstract A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of Streptococcus pneumoniae. Plasmid pSM22, the vector containing the pneumocccal polA gene, facilitates the expression of 50-fold greater amounts of the PolI enzyme.
Inventors
- 👤 Sanford A. Lacks
- 👤 Susana Martinez
- 👤 Paloma Lopez
- 👤 Manuel Espinosa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 19:09
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1252
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
29 words
Plasmid pSM22, deposited in the American Type Culture Collection under ATCC No. 67496, which includes the polA gene of Streptococcus Pneumoniae encoding a DNA polymerase in a broad-host-range vector.
Claim 2
Dependent Claim
29 words
Plasmid pSM22, deposited in the American Type Culture Collection under ATCC No. 67496, which includes the polA gene of Streptococcus Pneumoniae encoding a DNA polymerase in a broad-host-range vector.
Claim 3
Dependent Claim
32 words
Plasmid pSM23, deposited in the American Type Culture Collection under ATCC No. 67498, which includes the polA gene of Streptococcus pneumoniae encoding a DNA polymerase in a high-expression vector of Escherichia coli.
Claim 4
Dependent Claim
32 words
Plasmid pSM23, deposited in the American Type Culture Collection under ATCC No. 67498, which includes the polA gene of Streptococcus pneumoniae encoding a DNA polymerase in a high-expression vector of Escherichia coli.
Claim 5
Dependent Claim
71 words
A process for the production of a DNA polymerase-exonuclease protein comprising: (a) generating a genomic library of Streptococcus pneumoniae using a multicopy streptococcal vector; (b) transforming bacterial host cells deficient in nucleases; (c) screening the transformed cells for exonuclease activity using a DNase colony assay; (d) isolating nuclease-positive clones containing the desired recombinant plasmid, producing a DNA polymerase-exonuclease protein; and (e) isolating said protein using an assay for detecting nuclease activity.
Claim 6
Dependent Claim
71 words
A process for the production of a DNA polymerase-exonuclease protein comprising: (a) generating a genomic library of Streptococcus pneumoniae using a multicopy streptococcal vector; (b) transforming bacterial host cells deficient in nucleases; (c) screening the transformed cells for exonuclease activity using a DNase colony assay; (d) isolating nuclease-positive clones containing the desired recombinant plasmid, producing a DNA polymerase-exonuclease protein; and (e) isolating said protein using an assay for detecting nuclease activity.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-03-25 | 1994-09-24 | 1995-03-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-03-25 | 1998-09-24 | 1999-03-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-03-25 | 2002-09-24 | 2003-03-26 | $7700.00 | Window Closed / Lapsed |