US5000854A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Protamine-based filter device for removal of heparin from blood samples
Assignee: BOARD OF REGENTS ACTING FOR AND ON BEHALF OF UNIVERSITY OF MICHIGAN A CORP OF MI
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Filing: 1989-06-14
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Granted: 1991-03-19
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Expires: 2009-06-14
Abstract
Abstract An arrangement for collecting blood from a source of blood employs a filter chamber having a substantially predefined volume for accumulating the collected blood, and first and second access ports for receiving the collected blood and for venting the filter chamber, respectively. A protamine filter is arranged within the filter chamber, and preferably is formed of regenerated cellulose hollow fibers which have been activated with CNBr and have protamine immobilized thereon. The amount of protamine immobilized thereon is controlled in a variety of ways, prior to installation and sealing of the filter element within the filter chamber. In a practical embodiment, the first port of the filter chamber is coupled to a conventional cannula needle for communicating transdermally with a living being or through a membrane of a blood storage container. The second port is coupled to a conventional syringe which applies a suction for facilitating withdrawal of the blood. In other embodiments, the protamine is immobilized on a biocompatible polymer substrate by covalent bonding.
Inventors
- 👤 Victor C. Yang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 17:00
CPC Classifications
A; A61; A61B; A61B5/00; A61B5/15; A61B5/153; A61B5/150007; A61B5/150015; A61B5/15003; A61B5/150206; A61B5/150213; A61B5/150374; A61B5/150381; A61B5/150389; A61B5/150503; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375; Y10T436/255
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
78 words
An arrangement for collecting a blood fluid, from a source thereof, the arrangement comprising: filter chamber means having a substantially predefined volume for accumulating the collected fluid, a first port for coupling to the source of fluid and receiving the collected fluid, and a second port for venting said filter chamber; and filter means formed of protamine immobilized on a support member, said filter means being disposed within said filter chamber means for communicating with said accumulated fluid.
Claim 2
Dependent Claim
78 words
An arrangement for collecting a blood fluid, from a source thereof, the arrangement comprising: filter chamber means having a substantially predefined volume for accumulating the collected fluid, a first port for coupling to the source of fluid and receiving the collected fluid, and a second port for venting said filter chamber; and filter means formed of protamine immobilized on a support member, said filter means being disposed within said filter chamber means for communicating with said accumulated fluid.
Claim 3
Dependent Claim
30 words
The arrangement of claim 1 wherein there is further provided cannula needle means coupled to said first port of said filter chamber means, for coupling with the source of fluid.
Claim 4
Dependent Claim
44 words
The arrangement of claim 1 wherein there is further provided suction means coupled to said second port of said filter chamber means for applying a negative pressure to said filter chamber means and drawing the fluid to be collected into said filter chamber means.
Claim 5
Dependent Claim
45 words
The arrangement of claim 3 wherein said suction means comprises: suction cylinder means coupled at one end thereof to said second port of said filter chamber means; and plunger means arranged in said suction cylinder means for sliding sealingly therewithin and thereby producing said suction.
Claim 6
Dependent Claim
19 words
The arrangement of claim 1 wherein said support member of said filter means is formed of cellulose hollow fiber.
Claim 7
Dependent Claim
20 words
The arrangement of claim 1 wherein said support member of said filter means is formed of a blood-compatible polymer matrix.
Claim 8
Dependent Claim
27 words
The arrangement of claim 6 wherein said support member is formed of a selectable one of polysulfone, polycarbonate, polyether, polyether carbonate, ethylene vinyl alcohol, nylon, and polyurethane.
Claim 9
Dependent Claim
18 words
The arrangement of claim 6 wherein said protamine is bound by covalent bonding to said blood-compatible polymer matrix.
Claim 10
Independent Claim
63 words
A method of collecting blood from a living being, the method comprising the steps of: coupling an inlet port of a filter chamber to the living being; applying a suction to a vent port of the filter chamber for drawing the blood into the filter chamber through said inlet port; and subjecting the blood to an immobilized protamine filter in said filter chamber.
Claim 11
Independent Claim
63 words
A method of collecting blood from a living being, the method comprising the steps of: coupling an inlet port of a filter chamber to the living being; applying a suction to a vent port of the filter chamber for drawing the blood into the filter chamber through said inlet port; and subjecting the blood to an immobilized protamine filter in said filter chamber.
Claim 12
Dependent Claim
21 words
The method of claim 9 wherein said step of coupling comprises the further step of communicating transdermally with the living being.
Claim 13
Dependent Claim
57 words
The method of claim 9 wherein prior to performing said step of subjecting the blood, there are provided the further steps of: activating inner walls of at least a bundle of hollow cellulose fibers with cyanogen bromide; installing said bundle of activated hollow cellulose fibers into said filter chamber; and subjecting said bundle to a protamine-containing solution.
Claim 14
Dependent Claim
67 words
A protamine filter for communicating with a fluid and removing therefrom a component containing a selectable one of heparin and nucleic acids, the protamine filter comprising: housing means for forming a filter chamber for accommodating the fluid, said filter chamber having first and second ports; and protamine support means having protamine immobilized thereon for removing the component, said protamine support means being disposed within said housing means.
Claim 15
Dependent Claim
67 words
A protamine filter for communicating with a fluid and removing therefrom a component containing a selectable one of heparin and nucleic acids, the protamine filter comprising: housing means for forming a filter chamber for accommodating the fluid, said filter chamber having first and second ports; and protamine support means having protamine immobilized thereon for removing the component, said protamine support means being disposed within said housing means.
Claim 16
Dependent Claim
28 words
The protamine filter of claim 12 wherein said protamine support means comprises a bundle of hollow cellulose fibers having an inner wall which is preactivated with cyanogen bromide.
Claim 17
Dependent Claim
70 words
The protamine filter of claim 12 wherein said fluid is blood obtained from a living being, and there are further provided: cannula needle means coupled to said first port of said housing means for communicating transdermally with the living being; and syringe means coupled to said second port of said filter housing, for drawing said blood from said living being through said cannula needle means and into said filter housing.
Claim 18
Dependent Claim
16 words
The protamine filter of claim 12 wherein said protamine support means comprises a blood-compatible polymer matrix.
Claim 19
Dependent Claim
28 words
The protamine filter of claim 15 wherein said blood-compatible polymer matrix comprises a selectable one of polyacrylonitrile, polysulfone, polycarbonate, polyether, polyether carbonate, ethylene vinyl alcohol, nylon, and polyurethane.
Claim 20
Dependent Claim
19 words
The protamine filter of claim 15 wherein said protamine is bound by covalent bonding to said blood-compatible polymer matrix.
Claim 21
Dependent Claim
20 words
The protamine filter of claim 12 further comprising seal means for fixing said protamine support means in said housing means.
Claim 22
Dependent Claim
151 words
An arrangement for collecting a blood fluid, from a source thereof, the arrangement comprising: filter chamber means having a substantially predefined volume for accumulating the collected fluid, a first port for coupling to the source of fluid and receiving the collected fluid, and a second port for venting said filter chamber; suction means coupled to said second port of said filter chamber means for applying a negative pressure to said filter chamber means and drawing the fluid to be collected into said filter chamber means, said suction means comprising suction cylinder means coupled at one end thereof to said second port of said filter chamber means and plunger means arranged in said suction cylinder means for sliding sealingly therewithin and thereby producing said suction; and filter means formed of protamine immobilized on a support member, said filter means being disposed within said filter chamber means for communicating with said accumulated fluid.
Claim 23
Dependent Claim
151 words
An arrangement for collecting a blood fluid, from a source thereof, the arrangement comprising: filter chamber means having a substantially predefined volume for accumulating the collected fluid, a first port for coupling to the source of fluid and receiving the collected fluid, and a second port for venting said filter chamber; suction means coupled to said second port of said filter chamber means for applying a negative pressure to said filter chamber means and drawing the fluid to be collected into said filter chamber means, said suction means comprising suction cylinder means coupled at one end thereof to said second port of said filter chamber means and plunger means arranged in said suction cylinder means for sliding sealingly therewithin and thereby producing said suction; and filter means formed of protamine immobilized on a support member, said filter means being disposed within said filter chamber means for communicating with said accumulated fluid.
Claim 24
Dependent Claim
30 words
The arrangement of claim 19 wherein there is further provided cannula needle means coupled to said first port of said filter chamber means, for coupling with the source of fluid.
Claim 25
Dependent Claim
120 words
A protamine filter for communicating with the blood of a living being and removing therefrom a component containing a selectable one of heparin and nucleic acids, the protamine filter comprising: housing means for forming a filter chamber for accommodating the blood fluid, said filter chamber having first and second ports; cannula needle means coupled to said first port of said filter housing, for communicating transdermally with the living being; syringe means coupled to said second port of said filter housing, for drawing said blood from said living being through said cannula needle means and into said housing means; and protamine support means having protamine immobilized thereon for removing the component, said protamine support means being disposed within said housing means.
Claim 26
Dependent Claim
120 words
A protamine filter for communicating with the blood of a living being and removing therefrom a component containing a selectable one of heparin and nucleic acids, the protamine filter comprising: housing means for forming a filter chamber for accommodating the blood fluid, said filter chamber having first and second ports; cannula needle means coupled to said first port of said filter housing, for communicating transdermally with the living being; syringe means coupled to said second port of said filter housing, for drawing said blood from said living being through said cannula needle means and into said housing means; and protamine support means having protamine immobilized thereon for removing the component, said protamine support means being disposed within said housing means.
References Cited (Backward Citations)
US3933652A
Cited
US4048064A
Cited
US4175182A
Cited
US4199502A
Cited
US4464165A
Cited
US4381004A
Cited
US4500309A
Cited
US4483825A
Cited
US4551435A
Cited
US4800016A
Cited
US4863611A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-03-18 | 1994-09-17 | 1995-03-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-03-18 | 1998-09-17 | 1999-03-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-03-18 | 2002-09-17 | 2003-03-19 | $7700.00 | Window Closed / Lapsed |