US4996298A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Marker for colorectal carcinoma and methods of detecting the same
Assignee: Beth Israel Deaconess Medical Center
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Filing: 1990-01-22
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Granted: 1991-02-26
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Expires: 2010-01-22
Abstract
Abstract A new marker for colorectal carcinoma has been discovered which is a goycoprotein having a molecular weight of approximately 160,000 daltons. Assay methods which can identify this marker are useful in detecting, diagnosing, and monitoring colorectal carcinoma, and in particular, carcinoma of the undifferentiated variety which heretofor were not readily detectible. For example, an assay which utilizes an antibody reacting to this glycoprotein marker is useful in a screening method for the detection and monitoring of colorectal carcinoma cells. Such an antibody can be included as part of a kit for screening a patient for colorectal carcinoma.
Inventors
- 👤 Ronald R. Salem
- 👤 Peter Thomas
- 👤 Glenn Steele
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 05:54
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/30; C07K16/3046; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/828
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
121 words
An isolated, partially purified colorectal carcinoma (CC) glycoprotein, said glycoprotein being characterized by: (a) a molecular weight of approximately 160,000 daltons as determined by electrophoresis on polyacrylamide gels containing sodium dodecyl sulfate; (b) reacting with an antibody which binds undifferentiated colorectal carcinoma cells; (c) being located on the surface of a colorectal carcinoma cell; (d) not cross-reacting with antibodies which bind carcinoembryonic antigen (CEA); (e) not cross-reacting with antibodies which bind sialyated Lewis a antigen; (f) not cross-reacting with antibodies which bind alpha-1-acid glycoprotein; (g) not cross-reacting with antibodies which bind nonspecific cross-reacting antigen (NCA); (h) not cross-reacting with antibodies which bind any of blood group substances A, B, or H; and (i) not found on cancerous pancreatic or lung cells.
Claim 2
Dependent Claim
121 words
An isolated, partially purified colorectal carcinoma (CC) glycoprotein, said glycoprotein being characterized by: (a) a molecular weight of approximately 160,000 daltons as determined by electrophoresis on polyacrylamide gels containing sodium dodecyl sulfate; (b) reacting with an antibody which binds undifferentiated colorectal carcinoma cells; (c) being located on the surface of a colorectal carcinoma cell; (d) not cross-reacting with antibodies which bind carcinoembryonic antigen (CEA); (e) not cross-reacting with antibodies which bind sialyated Lewis a antigen; (f) not cross-reacting with antibodies which bind alpha-1-acid glycoprotein; (g) not cross-reacting with antibodies which bind nonspecific cross-reacting antigen (NCA); (h) not cross-reacting with antibodies which bind any of blood group substances A, B, or H; and (i) not found on cancerous pancreatic or lung cells.
Claim 3
Dependent Claim
14 words
The glycoprotein of claim 1 wherein said protein is purified from colorectal carcinoma cells.
Claim 4
Dependent Claim
15 words
The glycoprotein of claim 2 wherein said protein is purified from undifferentiated colorectal carcinoma cells.
References Cited (Backward Citations)
US4363799A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-02-25 | 1994-08-27 | 1995-02-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-02-25 | 1998-08-27 | 1999-02-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-02-25 | 2002-08-27 | 2003-02-26 | $7700.00 | Window Closed / Lapsed |