US4983523A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Methods for preparing sample nucleic acids for hybridization
Assignee: BP Corp North America Inc
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Filing: 1988-04-08
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Granted: 1991-01-08
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Expires: 2008-04-08
Abstract
Abstract A new method for releasing sample nucleic acids from cells, bacteria and viruses comprises non-invasively sonicating the sample contained within a sample container brought into physical contact with the vibrating element of a sonicator tuned to resonate at a frequency of 40 KHz or greater.
Inventors
- 👤 May K. Li
- 👤 Donna McLaughlin
- 👤 Elaine Palome
- 👤 Jack Kessler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 02:09
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; B01L3/5082; C; C12; C12M; C12M47/00; C12M47/06; C12N; C12N1/00; C12N1/06; C12N1/066; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12Q; C12Q1/00; C12Q1/68; C12Q1/6806; Y; Y10; Y10S; Y10S435/00; Y10S435/803
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
132 words
A method for treating an aqueous sample containing microorganisms for releasing sample nucleic acids contained within the microorganisms for hybridization comprising the steps of: (a) providing a non-invasive sonicator having a vibrating element tuned to resonate at a frequency of at least of 40 KHz but less than 100 KHz; (b) further providing a container for receiving said aqueous sample, said container having at least one mating surface adapted for engaging with said vibrating element; (c) adding said aqueous sample to said container; (d) bringing said mating surface of said container into contact with said vibrating element of said sonicator while avoiding immersion of said vibrating element in said aqueous sample; (e) energizing said sonicator to cause said vibrating element to vibrate for a time period of at least about 10 seconds.
Claim 2
Independent Claim
132 words
A method for treating an aqueous sample containing microorganisms for releasing sample nucleic acids contained within the microorganisms for hybridization comprising the steps of: (a) providing a non-invasive sonicator having a vibrating element tuned to resonate at a frequency of at least of 40 KHz but less than 100 KHz; (b) further providing a container for receiving said aqueous sample, said container having at least one mating surface adapted for engaging with said vibrating element; (c) adding said aqueous sample to said container; (d) bringing said mating surface of said container into contact with said vibrating element of said sonicator while avoiding immersion of said vibrating element in said aqueous sample; (e) energizing said sonicator to cause said vibrating element to vibrate for a time period of at least about 10 seconds.
Claim 3
Dependent Claim
42 words
The method as provided in claim 1 wherein said container through said mating surface is brought into contact with said vibrating element sonicator with a force of at least about 8 pounds per square inch to about 30 pounds per square inch.
Claim 4
Dependent Claim
19 words
The method as provided in claim 1 where said sonicator is tuned to a frequency of about 60 KHz.
Claim 5
Dependent Claim
35 words
The method as provided in claim 3 wherein said sonicator has a positive duty cycle of between about 25% to about 75% and said energizing step occurs from about 30 seconds to about 5 minutes.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-01-07 | 1994-07-09 | 1995-01-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-01-07 | 1998-07-09 | 1999-01-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-01-07 | 2002-07-09 | 2003-01-08 | $7700.00 | Window Closed / Lapsed |