US4981803A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reagent for reticulocyte counting by flow cytometry
Assignee: Sysmex Corp
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Filing: 1987-11-04
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Granted: 1991-01-01
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Expires: 2007-11-04
Abstract
Abstract A reagent for reticulocyte counting by flow cytometry which comprises two solutions, namely, a stock solution for staining in which a dye is dissolved in a nonaqueous solvent, and a buffer solution which satisfies the optimum staining conditions. By combining these two solutions immediately before measurement, a stable final staining solution for reticulocyte counting can always be obtained.
Inventors
- 👤 Tomoyuki Kuroda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 23:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/80; G01N33/5005; G01N33/5094; Y; Y10; Y10T; Y10T436/00; Y10T436/13
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
67 words
A kit for preparing a reagent for reticulocyte counting by flow cytometry which kit comprises a first, nonaqueous, solution and a second, aqueous, solution, said first solution comprising a nonaqueous solvent selected from the group consisting of ethylene glycol, triethylene glycol, and diethylene glycol, and a fluorescent diphenylmethane dye capable of dyeing a reticulocyte; and said second solution being an aqueous buffer solution suitable for staining reticulocytes.
Claim 2
Dependent Claim
67 words
A kit for preparing a reagent for reticulocyte counting by flow cytometry which kit comprises a first, nonaqueous, solution and a second, aqueous, solution, said first solution comprising a nonaqueous solvent selected from the group consisting of ethylene glycol, triethylene glycol, and diethylene glycol, and a fluorescent diphenylmethane dye capable of dyeing a reticulocyte; and said second solution being an aqueous buffer solution suitable for staining reticulocytes.
Claim 3
Dependent Claim
29 words
A reagent according to claim 1 in which the buffer solution is a member of the group consisting of HEPES buffer solution, phosphate buffer solution or Tris-Tricine buffer solution.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-12-31 | 1994-07-02 | 1995-01-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-12-31 | 1998-07-02 | 1999-01-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-12-31 | 2002-07-02 | 2003-01-01 | $7700.00 | Window Closed / Lapsed |