US4975528A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for separation and concentration of apolipoproteins using perfluorocarbon emulsion
Assignee: Affinity Biotech Inc
•
Filing: 1989-10-16
•
Granted: 1990-12-04
•
Expires: 2009-10-16
Abstract
Abstract Lipoproteins are separated from their aqueous solutions, e.g., blood, by treatment with PFC emulsified with phospholipid and then separating the emulsion containing the apolipoproteins. The latter are readily separated from the emulsion.
Inventors
- 👤 Edward A. Kaminski
- 👤 Albert J. Owen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 15:10
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/775
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
50 words
Method of separating lipoprotein present in an aqueous solution thereof which comprises mixing the solution with an emulsion of perfluorocarbon particles in water containing phospholipid, whereby the protein portion of said lipoprotein binds to phospholipid on the surface of the perfluorocarbon particles, and separating phospholipid-protein-containing perfluorocarbon from said aqueous solution.
Claim 2
Dependent Claim
50 words
Method of separating lipoprotein present in an aqueous solution thereof which comprises mixing the solution with an emulsion of perfluorocarbon particles in water containing phospholipid, whereby the protein portion of said lipoprotein binds to phospholipid on the surface of the perfluorocarbon particles, and separating phospholipid-protein-containing perfluorocarbon from said aqueous solution.
Claim 3
Dependent Claim
22 words
Method according to claim 1 wherein said separated perfluorocarbon is further treated with aqueous ionic or nonionic surfactant to separate protein therefrom.
Claim 4
Dependent Claim
13 words
Method according to claim 1 wherein said aqueous solution is blood or plasma.
Claim 5
Dependent Claim
17 words
Method according to claim 1 wherein the separated fluorocarbon is further treated to identify the proteins therein.
Claim 6
Dependent Claim
14 words
Method according to claim 1 wherein the emulsion additionally contains lysophospholipid or phosphatidic acid.
Claim 7
Dependent Claim
11 words
Method according to claim 5 wherein the emulsion additionally contains lysophospholipid.
Claim 8
Dependent Claim
12 words
Method according to claim 5 wherein the emulsion additionally contains phosphatidic acid.
Claim 9
Dependent Claim
29 words
Method according to claim 1, 2, 3, 4 and 5 wherein the aqueous phase of said emulsion contains less than 35% of the amount of phospholipid in said emulsion.
Claim 10
Dependent Claim
23 words
Method according to claim 3 wherein ionic or nonionic surfactant is added to the blood to improve said binding of protein to phospholipid.
Claim 11
Dependent Claim
30 words
Method according to claims 1, 3 or 4 wherein the perfluorocarbon is a fluorohydrocarbon and at least 80% of the hydrogen atoms in the hydrocarbon have been replaced with fluorine.
Claim 12
Dependent Claim
30 words
Method according to claims 1, 3 or 4 wherein the perfluorocarbon is a fluorohydrocarbon and at least 99% of the hydrogen atoms in the hydrocarbon have been replaced with fluorine.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-12-03 | 1994-06-04 | 1994-12-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-12-03 | 1998-06-04 | 1998-12-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-12-03 | 2002-06-04 | 2002-12-04 | $7700.00 | Window Closed / Lapsed |