US4971901A
Expired / Lapsed
US
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Process for preparing enzyme electrodes
Assignee: New Oji Paper Co Ltd
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Filing: 1988-01-20
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Granted: 1990-11-20
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Expires: 2008-01-20
Abstract
Abstract An enzyme electrode is prepared by a process wherein an albumin solution containing 0.05 to 0.5% by weight of a multifunctional aldehyde is applied onto the surface of an electroconductive substrate while maintaining the atmospheric temperature at 0° to 20° C. to thereby form a crosslinked albumin layer on the surface of the electroconductive substrate. Thereafter a solution containing 0.5% by weight or less of a multifunctional aldehyde and a predetermined amount of an oxidase capable of producing hydrogen peroxide (H 2 O 2 ) is applied on the surface of the previously formed crosslinked albumin layer to form a H 2 O 2 productive oxidase containing layer.
Inventors
- 👤 Ryuzo Hayashi
- 👤 Akio Kariyone
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 12:39
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
106 words
A process for preparing an enzyme electrode consisting essentially of the steps of: (a) applying an albumin solution containing 0.05 to 0.5% by weight of a multifunctional aldehyde onto the surface of an electroconductive substrate to form a coat comprised of said solution, (b) maintaining the atmospheric temperature of 0° C. to 20° C. to thereby form a permselective crosslinked albumin layer, and (c) applying a solution containing a hydrogen peroxide productive oxidase and greater than 0 up to 0.5% by weight of a multifunctional aldehyde onto the surface of the above permselective crosslinked albumin layer to form a hydrogen peroxide productive oxidase containing layer thereon.
Claim 2
Dependent Claim
106 words
A process for preparing an enzyme electrode consisting essentially of the steps of: (a) applying an albumin solution containing 0.05 to 0.5% by weight of a multifunctional aldehyde onto the surface of an electroconductive substrate to form a coat comprised of said solution, (b) maintaining the atmospheric temperature of 0° C. to 20° C. to thereby form a permselective crosslinked albumin layer, and (c) applying a solution containing a hydrogen peroxide productive oxidase and greater than 0 up to 0.5% by weight of a multifunctional aldehyde onto the surface of the above permselective crosslinked albumin layer to form a hydrogen peroxide productive oxidase containing layer thereon.
Claim 3
Dependent Claim
24 words
The process according to claim 1, wherein the albumin solution in the step (a) contains 0.1 to 0.5% by weight of a multifunctional aldehyde.
Claim 4
Dependent Claim
20 words
The process of claim 1, wherein the step (b) is carried out at a temperature of 0 to 10° C.
Claim 5
Dependent Claim
12 words
The process according to claim 1, wherein the electroconductive substrate is platinum.
Claim 6
Dependent Claim
19 words
The process according to claim 4, wherein the platinum substrate has a surface treated with a silane coupling reagent.
Claim 7
Dependent Claim
29 words
The process according to claim 5, wherein the silane coupling reagent is one or more members selected from the group consisting of γ-aminopropyltriethoxy silane, 4-aminobutyldimethylmethoxy silane and 4-aminobutyltriethoxy silane.
Claim 8
Dependent Claim
52 words
The process according to claim 5, wherein prior to subjecting to the silane coupling reagent treatment, the platinum substrate is subjected to electrolytic treatment using the platinum substrate as the working electrode at an electric potential of +1.0 to +1.5 V νs Ag/AgCl electrode for a period of more than 30 seconds.
Claim 9
Dependent Claim
12 words
The process according to claim 1, wherein the multifunctional aldehyde is glutaraldehyde.
Claim 10
Dependent Claim
13 words
The process according to claim 1, wherein the albumin is bovine serum albumin.
Claim 11
Dependent Claim
30 words
The process according to claim 1, wherein the step (b) is carried out at a temperature of 0 to 10° C. for a period of 20 minutes to 24 hours.
Claim 12
Dependent Claim
15 words
The process according to claim 10, wherein the period is 30 minutes to 4 hours.
Claim 13
Dependent Claim
18 words
The process according to claim 1, wherein the hydrogen peroxide productive oxidase is selected from glucoseoxidase and galactoseoxidase.
Claim 14
Dependent Claim
21 words
The process according to claim 1, wherein the solution in the step (c) further contains a protein other than an enzyme.
Claim 15
Dependent Claim
11 words
The process according to claim 13, wherein the protein is albumin.
Claim 16
Dependent Claim
13 words
An enzyme electrode produced in accordance with the process according to claim 1.
References Cited (Backward Citations)
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US4229536A
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US4476005A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-11-19 | 1994-05-21 | 1994-11-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-11-19 | 1998-05-21 | 1998-11-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-11-19 | 2002-05-21 | 2002-11-20 | $7700.00 | Window Closed / Lapsed |