US4968613A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Luciferase gene and novel recombinant DNA as well as a method of producing luciferase
Assignee: Kikkoman Corp
•
Filing: 1988-07-26
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Granted: 1990-11-06
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Expires: 2008-07-26
Abstract
Abstract A luciferase gene isolated from Luciola cruciata (Japanese firefly) coding for an amino acid sequence shown in FIG. 4 and a novel recombinant DNA characterized by incorporating a gene coding for luciferase into a vector DNA are disclosed. There is also disclosed a method of producing luciferase which comprises culturing in a medium a microorganism containing a recombinant DNA having inserted a gene coding for luciferase in a vector DNA and belonging to the genus Escherichia capable of producing luciferase and collecting luciferase from the culture.
Inventors
- 👤 Tsutomu Masuda
- 👤 Hiroki Tatsumi
- 👤 Eiichi Nakano
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 18:25
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0069; C12Y; C12Y113/00; C12Y113/12; C12Y113/12007
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
14 words
An isolated luciferase gene coding for an amino acid sequence shown in FIG. 4.
Claim 2
Dependent Claim
14 words
An isolated luciferase gene coding for an amino acid sequence shown in FIG. 4.
Claim 3
Dependent Claim
15 words
An isolated luciferase gene according to claim 1, having a DNA sequence of FIG. 3.
Claim 4
Dependent Claim
11 words
A recombinant DNA comprising an isolated luciferase DNA from Luciola cruciata.
Claim 5
Dependent Claim
11 words
A recombinant DNA comprising an isolated luciferase DNA from Luciola cruciata.
Claim 6
Dependent Claim
20 words
A recombinant DNA according to claim 3 wherein said isolated luciferase DNA has a nucleotide sequence shown in FIG. 3.
Claim 7
Dependent Claim
21 words
A recombinant DNA according to claim 3 wherein said isolated luciferase DNA codes for an amino acid sequence of FIG. 4.
Claim 8
Dependent Claim
21 words
A recombinant DNA according to claim 3, wherein said isolated luciferase DNA from Luciola cruciata is inserted into plasmid pUC18 DNA.
Claim 9
Independent Claim
44 words
A method of producing luciferase which comprises culturing, in a medium, a microorganism belonging to the genus Escherichia capable of producing luciferase, said microorganism containing a recombinant DNA having inserted therein an isolated luciferase gene from Luciola cruciata and collecting luciferase from the culture.
Claim 10
Independent Claim
44 words
A method of producing luciferase which comprises culturing, in a medium, a microorganism belonging to the genus Escherichia capable of producing luciferase, said microorganism containing a recombinant DNA having inserted therein an isolated luciferase gene from Luciola cruciata and collecting luciferase from the culture.
Claim 11
Dependent Claim
22 words
A method of producing luciferase according to claim 7, wherein said gene codes for an amino acid sequence shown in FIG. 4.
Claim 12
Dependent Claim
20 words
A method of producing luciferase according to claim 7 wherein said gene has a nucleotide sequence shown in FIG. 3.
Claim 13
Dependent Claim
28 words
A method for producing luciferase according to claim 7, wherein said recombinant DNA is one produced by inserting an isolated luciferase gene from Luciola cruciata into pUC18 DNA.
References Cited (Backward Citations)
US4581335A
Cited
WO1987003304A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-11-05 | 1994-05-07 | 1994-11-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-11-05 | 1998-05-07 | 1998-11-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-11-05 | 2002-05-07 | 2002-11-06 | $7700.00 | Window Closed / Lapsed |