US4968600A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Apparatus for separating cell suspension
Assignee: Toray Industries Inc
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Filing: 1987-03-13
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Granted: 1990-11-06
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Expires: 2007-03-13
Abstract
Abstract An apparatus for fractionating a cell suspension, which comprises a casing having a substantially columnar space in the interior, which is substantially closed to the space outside the casing, a liquid-treating chamber in the casing adapted to be filled with a cell suspension passing through this liquid-treating chamber, a separating membrane having a separating wall surface inside the casing and disposed to separate and cause a cell component to pass through the separating membrane, a rotary body rotatably arranged in the liquid-treating chamber, a driving mechanism for rotating and driving the rotary body, a cell suspension introduction path, a treated liquid discharge path, and a separated component discharge path.
Inventors
- 👤 Masato Haraguchi
- 👤 Yuichi Mori
- 👤 Ichiro Itagaki
- 👤 Masao Hirose
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 16:29
CPC Classifications
B; B01; B01D; B01D63/00; B01D63/16; A; A61; A61M; A61M1/00; A61M1/34; B01D29/00; B01D29/01; B01D29/018; B01D29/50; B01D29/52; B01D29/54; B01D29/76; B01D29/86; B01D33/00; B01D33/15; B01D33/21; B01D33/35; B01D33/37; B01D33/39; B01D33/58; B01D33/68; B01D63/08; B01D63/087; B01D65/00; B01D65/08; B01D2321/00; B01D2321/16; B01D2321/168; B01D2321/20; B01D2321/2008; B01D2321/2016; Y; Y10; Y10S; Y10S210/00; Y10S210/929
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
195 words
An apparatus for fractionating a cell suspension which comprises: (a) a casing defining an interior space which is substantially closed to the space outside said casing, said interior space comprising a separating chamber disposed in said casing and adapted to contain a cell suspension and including a separated component chamber, (b) a separating membrane having a separating surface which is interposed in said casing between the separating chamber and the separated component chamber to separate a predetermined component from said cell suspension and to cause said component to pass therethrough, (c) a shearing wall body disposed in said casing having a shearing surface confronting the separating surface of said separating membrane, (d) drive means connected to cause relative rotation of said shearing body and said separating surface to apply a shearing force to said cell suspension between said separating membrane and said shearing surface, (e) means forming a liquid introduction path to introduce liquid to said separating chamber, (f) means forming a treated liquid discharge path to remove treated liquid from said separating chamber, and (g) means forming a separated component liquid discharge path connected to the separated component chamber to deliver the separated component.
Claim 2
Independent Claim
195 words
An apparatus for fractionating a cell suspension which comprises: (a) a casing defining an interior space which is substantially closed to the space outside said casing, said interior space comprising a separating chamber disposed in said casing and adapted to contain a cell suspension and including a separated component chamber, (b) a separating membrane having a separating surface which is interposed in said casing between the separating chamber and the separated component chamber to separate a predetermined component from said cell suspension and to cause said component to pass therethrough, (c) a shearing wall body disposed in said casing having a shearing surface confronting the separating surface of said separating membrane, (d) drive means connected to cause relative rotation of said shearing body and said separating surface to apply a shearing force to said cell suspension between said separating membrane and said shearing surface, (e) means forming a liquid introduction path to introduce liquid to said separating chamber, (f) means forming a treated liquid discharge path to remove treated liquid from said separating chamber, and (g) means forming a separated component liquid discharge path connected to the separated component chamber to deliver the separated component.
Claim 3
Independent Claim
169 words
An apparatus for fractionating a cell suspension which comprises: (a) a casing defining an interior space which is substantially closed to the space outside said casing, said interior space comprising a separating chamber disposed of in said casing and adapted to contain a cell suspension and including a separated component chamber, (b) a separating membrane having a separating surface which is interposed in said casing between the separating chamber and the separated component chamber to separate a predetermined component from said cell suspension and to cause said component to pass therethrough, (c) a rotary body rotatably disposed in said casing having a shearing surface confronting the separating surface of said separating membrane, (d) drive means connected to rotate and drive said rotary body, (e) means forming a liquid introduction path connected to said separating chamber, (f) means forming a treated liquid discharge path connected to said separating chamber, and (g) means forming a separated component liquid discharge path connected to the separated component chamber to deliver the separated component.
Claim 4
Independent Claim
169 words
An apparatus for fractionating a cell suspension which comprises: (a) a casing defining an interior space which is substantially closed to the space outside said casing, said interior space comprising a separating chamber disposed of in said casing and adapted to contain a cell suspension and including a separated component chamber, (b) a separating membrane having a separating surface which is interposed in said casing between the separating chamber and the separated component chamber to separate a predetermined component from said cell suspension and to cause said component to pass therethrough, (c) a rotary body rotatably disposed in said casing having a shearing surface confronting the separating surface of said separating membrane, (d) drive means connected to rotate and drive said rotary body, (e) means forming a liquid introduction path connected to said separating chamber, (f) means forming a treated liquid discharge path connected to said separating chamber, and (g) means forming a separated component liquid discharge path connected to the separated component chamber to deliver the separated component.
Claim 5
Independent Claim
188 words
An apparatus for fractionating a cell suspension which comprises: (a) a casing having a substantially columnar space in the interior thereof defining an interior space which is substantially closed to the space outside said casing, said interior space comprising a separating chamber disposed in said casing and adapted to contain a cell suspension, (b) means in said space having a shearing surface, (c) a rotary body rotatably disposed in said casing having a separating membrane in said space having a separating surface which confronts said shearing surface, said membrane being disposed to separate a predetermined component from said cell suspension and to cause said component to pass therethrough, and having a separated component chamber in said space adjacent said separating membrane, (d) a driving mechanism engaged to rotate and drive said rotary body, (e) means forming a liquid introduction path connected to said separating chamber for introduction of liquid to be treated, (f) a trated liquid discharge path connected to said separating chamber for delivery of treated liquid, and (g) means forming a separated component liquid path connected to said component chamber for delivery of said separated component.
Claim 6
Independent Claim
188 words
An apparatus for fractionating a cell suspension which comprises: (a) a casing having a substantially columnar space in the interior thereof defining an interior space which is substantially closed to the space outside said casing, said interior space comprising a separating chamber disposed in said casing and adapted to contain a cell suspension, (b) means in said space having a shearing surface, (c) a rotary body rotatably disposed in said casing having a separating membrane in said space having a separating surface which confronts said shearing surface, said membrane being disposed to separate a predetermined component from said cell suspension and to cause said component to pass therethrough, and having a separated component chamber in said space adjacent said separating membrane, (d) a driving mechanism engaged to rotate and drive said rotary body, (e) means forming a liquid introduction path connected to said separating chamber for introduction of liquid to be treated, (f) a trated liquid discharge path connected to said separating chamber for delivery of treated liquid, and (g) means forming a separated component liquid path connected to said component chamber for delivery of said separated component.
Claim 7
Dependent Claim
27 words
A cell suspension-fractionating apparatus as set forth in claim 1 or 3, wherein the cell suspension is blood and the predetermined component to be separated is plasma.
Claim 8
Dependent Claim
28 words
A cell suspension-fractionating apparatus as set forth in claim 1 or 3, wherein the average diameter of the pores of the separating membrane is 0.2 to 1.0 μm.
Claim 9
Dependent Claim
26 words
A cell suspension-fractionating apparatus as set forth in claim 4, wherein the average diameter of the pores of the separating membrane is 0.4 to 0.8 μm.
Claim 10
Dependent Claim
21 words
A cell suspension-fractionating apparatus as set forth in claim 1 or 3, wherein the separating membrane is composed of a polycarbonate.
Claim 11
Dependent Claim
23 words
A cell suspension-fractionating apparatus as set forth in claim 1 or 3, wherein the separating membrane is composed of a cellulose mixed ester.
Claim 12
Dependent Claim
20 words
A cell suspension-fractionating apparatus as set forth in claim 1 or 3, wherein the separating membrane is composed of polyethylene.
Claim 13
Dependent Claim
21 words
A cell suspension-fractionating apparatus as set forth in claim 1 or 3, wherein the separating membrane is composed of a polysulfone.
Claim 14
Dependent Claim
22 words
A cell suspension-fractionating apparatus as set forth in claim 1- or 3, wherein the separating membrane is composed of a ceramic material.
Claim 15
Dependent Claim
26 words
A cell suspension-fractionating apparatus as set forth in claim 1 or 3, wherein the area of the separating surface is 10 to 100 cm 2 .
Claim 16
Dependent Claim
25 words
A cell suspension-fractionating apparatus as set forth in claim-1 or 3, wherein each of the separating surface and the shearing surface is a plane surface.
Claim 17
Dependent Claim
26 words
A cell suspension-fractionating apparatus as set forth in claim 13, wherein the distance between the separating surface and the shearing surface in 0.05 to 2 mm.
Claim 18
Dependent Claim
23 words
A cell suspension-fractionating apparatus as set forth in claim 14, wherein the outer diameter of the shearing surface is 30 to 100 mm.
Claim 19
Dependent Claim
31 words
A cell suspension-fractionating apparatus as set forth in claim 1 or 3, wherein one of the separating surface and the shearing surface is a conical surface convex to the other surface.
Claim 20
Dependent Claim
26 words
A cell suspension-fractionating apparatus as set forth in claim 16, wherein the crossing angle between the separating surface and the shearing surface is 0.5° to 5°.
Claim 21
Dependent Claim
26 words
A cell suspension-fractionating apparatus as set forth in claim 17, wherein the crossing angle between the separating surface and the shearing surface is 0.8° to 3°.
Claim 22
Dependent Claim
23 words
A cell suspension-fractionating apparatus as set forth in claim 16, wherein the outer diameter of the shearing surface is 20 to 100 mm.
Claim 23
Dependent Claim
18 words
A cell suspension-fractionating apparatus as set forth in claim 16, wherein the separating surface is a plane surface.
Claim 24
Dependent Claim
31 words
A cell suspension-fractionating apparatus as set forth in claim 1 or 3, wherein each of the separating surface and the shearing surface is a conical surface convex to the other surface.
Claim 25
Dependent Claim
26 words
A cell suspension-fractionating apparatus as set forth in claim 21, wherein the crossing angle between the separating surface and the shearing surface is 0.5° to 5°.
Claim 26
Dependent Claim
26 words
A cell suspension-fractionating apparatus as set forth in claim 22, wherein the crossing angle between the separating surface and the shearing surface is 0.8° to 3°.
Claim 27
Dependent Claim
23 words
A cell suspension-fractionating apparatus as set forth in claim 21, wherein the outer diameter of the shearing surface is 20 to 100 mm.
Claim 28
Dependent Claim
23 words
A cell suspension-fractionating apparatus as set forth in claim 1 or 3, wherein one separating surface and one shearing wall surface are disposed.
Claim 29
Dependent Claim
22 words
A cell suspension-fractionating apparatus as set forth in claim 1 or-3, wherein two separating wall surfaces and two shearing surfaces are disposed.
Claim 30
Dependent Claim
26 words
A cell suspension-fractionating apparatus as set forth in claim 26, wherein one separating surface is arranged on each of both surfaces of one separated component chamber.
Claim 31
Dependent Claim
25 words
A cell suspension-fractionating apparatus as set forth in claim 26, wherein one separating surface is arranged on each of both surfaces of one separating chamber.
Claim 32
Dependent Claim
50 words
A cell suspension-fractionating apparatus as set forth in claim 1 or 3, wherein the driving mechanism for rotating and driving the rotary body comprises a driving shaft attached to the rotary body and extended to the outside of the casing and a motor for rotating and driving the driving shaft.
Claim 33
Dependent Claim
55 words
A cell suspension-fractionating apparatus as set forth in claim 1 or 3, wherein the driving mechanism for rotating and driving the rotary body comprises a magnetic coupling member mounted on the rotary body and means arranged outside the casing and magnetically connected to the magnetic coupling member to rotate and drive the magnetic coupling member
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-11-05 | 1994-05-07 | 1994-11-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-11-05 | 1998-05-07 | 1998-11-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-11-05 | 2002-05-07 | 2002-11-06 | $7700.00 | Window Closed / Lapsed |