US4963658A
Expired / Lapsed
US
⏱️ 0.0 years remaining
DNA detection method
Assignee: Molecular Devices LLC
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Filing: 1988-12-15
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Granted: 1990-10-16
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Expires: 2008-12-15
Abstract
Abstract Picogram amounts of DNA can be detected in a sample by the use of high-affinity, single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.
Inventors
- 👤 Viola T. Kung
- 👤 Peter A. Nagainis
- 👤 Edward L. Sheldon, III
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 17:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5308; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6804; C12Q1/6813; C12Q1/6816; C12Q1/6834
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
37 words
A conjugate comprising a high affinity single-stranded DNA binding protein (BP) covalently linked to a detectable label (dl), wherein said conjugate is capable of binding with high affinity via said BP directly to DNA to be detected.
Claim 2
Dependent Claim
37 words
A conjugate comprising a high affinity single-stranded DNA binding protein (BP) covalently linked to a detectable label (dl), wherein said conjugate is capable of binding with high affinity via said BP directly to DNA to be detected.
Claim 3
Dependent Claim
21 words
The conjugate according to claim 1, wherein said BP is single-stranded DNA binding protein, T4 gene 32 protein, or topoisomerase I.
Claim 4
Dependent Claim
14 words
The conjugate according to claim 2, wherein said enzyme is urease or horseradish peroxidase.
Claim 5
Dependent Claim
12 words
The conjugate according to claim 1, wherein said dl is an enzyme.
Claim 6
Dependent Claim
39 words
A conjugate comprising a high affinity single-stranded DNA binding protein (BP) covalently linked to a specific binding pair member (sbpm), wherein said conjugate is capable of binding with high affinity via said BP directly to DNA to be detected.
Claim 7
Dependent Claim
39 words
A conjugate comprising a high affinity single-stranded DNA binding protein (BP) covalently linked to a specific binding pair member (sbpm), wherein said conjugate is capable of binding with high affinity via said BP directly to DNA to be detected.
Claim 8
Dependent Claim
15 words
The conjugate according to claim 5, wherein said sbpm is biotin, avidin, streptavidin or antibiotin.
References Cited (Backward Citations)
US4493899A
Cited
GB2125964A
Cited
EP0131830A1
Cited
EP0135159A2
Cited
EP0147665A1
Cited
WO1985005685A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-10-15 | 1994-04-16 | 1994-10-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-10-15 | 1998-04-16 | 1998-10-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-10-15 | 2002-04-16 | 2002-10-16 | $7700.00 | Window Closed / Lapsed |