US4963481A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Promoter system
Assignee: Genetics Institute LLC
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Filing: 1986-11-12
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Granted: 1990-10-16
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Expires: 2006-11-12
Abstract
Abstract Recombinant DNA molecules are disclosed which contain a nucleotide sequence encoding a mouse cytomegalovirus-derived transcriptional promoter. Eucaryotic host cells transformed with recombinant DNA molecules having such mouse cytomegalovirus-derived promoter sequences operatively linked to heterologous protein-coding sequences may be cultured as a method for producing the heterologous proteins or as a method for identifying and isolating DNA encoding the heterologous protein via any method involving detection of the protein.
Inventors
- 👤 Jean P. deVilliers
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 16:22
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
57 words
A hybrid transcription unit comprising a recombinant DNA molecule or which comprises a nucleotide sequence encoding a heterologous protein operatively linked to the approximately 2.27 kilobase pair PstI restriction fragment of the mCMV genome containing the mouse cytomegalovirus (mCMV) immediate early promoter or to an effective expression promoting fragment thereof functionally linked to a heterologous transcriptional enhancer.
Claim 2
Dependent Claim
57 words
A hybrid transcription unit comprising a recombinant DNA molecule or which comprises a nucleotide sequence encoding a heterologous protein operatively linked to the approximately 2.27 kilobase pair PstI restriction fragment of the mCMV genome containing the mouse cytomegalovirus (mCMV) immediate early promoter or to an effective expression promoting fragment thereof functionally linked to a heterologous transcriptional enhancer.
Claim 3
Dependent Claim
18 words
A hybrid transcription unit of claim 1 wherein the heterologous enhancer comprises the SV40 or polyomavirus transcriptional enhancer.
Claim 4
Dependent Claim
12 words
A eucaryotic host cell containing the hybrid transcription unit of claim 1.
Claim 5
Dependent Claim
12 words
A eucaryotic host cell containing the hybrid transcription unit of claim 2.
Claim 6
Dependent Claim
24 words
A method for producing a heterologous protein which comprises culturing host cells of claim 4 under suitable conditions permitting cell growth and gene expression.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-10-15 | 1994-04-16 | 1994-10-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-10-15 | 1998-04-16 | 1998-10-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-10-15 | 2002-04-16 | 2002-10-16 | $7700.00 | Window Closed / Lapsed |