US4963477A
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Probe containing a modified nucleic acid, recognizable by specific antibodies and use of this probe and theses specific antibodies to detect and characterize a homologous DNA sequence
Assignee: Institut National de la Santé et de la Recherche Médicale
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Filing: 1989-03-28
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Granted: 1990-10-16
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Expires: 2009-03-28
Abstract
Abstract The invention relates to a kit for detecting the presence of a nucleic acid sequence, such as a gene or a gene fragment, in a composition or a specimen supposed to contain it. The kit comprises a probe containing a nucleic acid complementary with the nucleic acid sequence or gene which is sought. The probe bears at least one 7-iodo-N-2-acetylamino-fluorene group covalently fixed at one at least of the bases of this probe.
Inventors
- 👤 Paul Tchen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 21:36
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6804; Y; Y10; Y10S; Y10S435/00; Y10S435/975; Y10S436/00; Y10S436/80; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
76 words
A kit for the detection or isolation of a first predetermined nucleotide sequence, comprising: a probe containing a second nucleotide sequence which is complementary to said first predetermined nucleotide sequence and which can be hybridized with said first predetermined nucleotide sequence; said probe further containing a 7-iodo-N-2-acetylaminofluorene group covalently fixed to a base of said second complementary nucleotide sequence; and first antibodies formed against N-2-(guanosine-8-yl)acetylaminofluorene or against a nucleotide sequence convalently fixed to an N-2-acetylaminofluorene group.
Claim 2
Dependent Claim
76 words
A kit for the detection or isolation of a first predetermined nucleotide sequence, comprising: a probe containing a second nucleotide sequence which is complementary to said first predetermined nucleotide sequence and which can be hybridized with said first predetermined nucleotide sequence; said probe further containing a 7-iodo-N-2-acetylaminofluorene group covalently fixed to a base of said second complementary nucleotide sequence; and first antibodies formed against N-2-(guanosine-8-yl)acetylaminofluorene or against a nucleotide sequence convalently fixed to an N-2-acetylaminofluorene group.
Claim 3
Dependent Claim
14 words
The kit of claim 1, which further comprises means for visualizing said first antibodies.
Claim 4
Dependent Claim
28 words
The kit of claim 2, wherein said visualizing means comprise second antibodies or polypeptides which are capable of reacting with said first antibodies and which bear a marker.
Claim 5
Dependent Claim
15 words
The kit of claim 3, wherein said marker is an enzyme or a fluorescent molecule.
Claim 6
Dependent Claim
15 words
The kit of claim 1, wherein said first and second nucleotide sequences are DNA sequences.
Claim 7
Dependent Claim
82 words
A kit for the detection or isolation of a first predetermined nucleotide sequence, comprising: a probe containing a second nucleotide sequence which is complementary to said first predetermined nucleotide sequence and which can be hybridized with said first predetermined nucleotide sequence; said probe further containing a 7-iodo-N-acetoxy-N-2-acetylaminofluorene group covalently fixed to a base of said second complementary nucleotide sequence; first antibodies formed against N-2-(guanosine-8-yl)-acetylaminofluorene or against a nucleotide sequence covalently fixed to an N-2-acetylaminofluorene group; and means for visualizing said first antibodies.
Claim 8
Dependent Claim
82 words
A kit for the detection or isolation of a first predetermined nucleotide sequence, comprising: a probe containing a second nucleotide sequence which is complementary to said first predetermined nucleotide sequence and which can be hybridized with said first predetermined nucleotide sequence; said probe further containing a 7-iodo-N-acetoxy-N-2-acetylaminofluorene group covalently fixed to a base of said second complementary nucleotide sequence; first antibodies formed against N-2-(guanosine-8-yl)-acetylaminofluorene or against a nucleotide sequence covalently fixed to an N-2-acetylaminofluorene group; and means for visualizing said first antibodies.
Claim 9
Dependent Claim
27 words
The kit of claim 6, wherein said visualizing means comprise second antibodies or polypeptides, which are capable of with said first antibodies and which bear a marker.
Claim 10
Dependent Claim
15 words
The kit of claim 7, wherein said first and second nucleotide sequences are DNA sequences.
Claim 11
Independent Claim
104 words
A method for detecting a first predetermined nucleotide sequence in a biological medium, comprising: contacting said medium, under hybridization conditions, with a prove containing a second nucleotide sequence which is complementary to said first predetermined nucleotide sequence and which can be hybridized with said first predetermined nucleotide sequence, said probe further containing a 7-iodo-N-2-acetylaminofluorene group covalently fixed to a base of said second complementary nucleotide sequence, to hybridize said probe to said first predetermined nucleotide sequence; and then contacting said hybridized probe and first predetermined nucleotide sequence with first antibodies formed against N-2-(guanosine-8yl)-acetylaminofluorene or against of nucleotide sequence covalently fixed to an N-2-acetylaminofluorene group.
Claim 12
Independent Claim
104 words
A method for detecting a first predetermined nucleotide sequence in a biological medium, comprising: contacting said medium, under hybridization conditions, with a prove containing a second nucleotide sequence which is complementary to said first predetermined nucleotide sequence and which can be hybridized with said first predetermined nucleotide sequence, said probe further containing a 7-iodo-N-2-acetylaminofluorene group covalently fixed to a base of said second complementary nucleotide sequence, to hybridize said probe to said first predetermined nucleotide sequence; and then contacting said hybridized probe and first predetermined nucleotide sequence with first antibodies formed against N-2-(guanosine-8yl)-acetylaminofluorene or against of nucleotide sequence covalently fixed to an N-2-acetylaminofluorene group.
Claim 13
Dependent Claim
28 words
The method of claim 9, which further comprises visualizing said first antibodies by reacting said first antibodies with a labeled antibody or labeled polypeptide to said first antibodies.
Claim 14
Dependent Claim
11 words
The method of claim 9, wherein the 7-iodo-N-2-acetylaminofluorene group is 7-iodo-N-acetoxy-N-2-acetylaminofluorene.
Claim 15
Dependent Claim
15 words
The method of claim 11, wherein the first antibodies are antibodies that immunologically recognize N-2-(guanosine-8-yl)-acetylaminofluorene.
References Cited (Backward Citations)
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