US4960330A
Expired / Lapsed
US
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Image recording apparatus
Assignee: Microscience Group Inc
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Filing: 1988-07-11
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Granted: 1990-10-02
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Expires: 2008-07-11
Abstract
Abstract Image recording apparatus comprising a block in which an object is embedded in fluorescence stained condition, means for holding said block, means for cutting slices from said block to form successively deeper faces on the block, and magnification and recording means, including a computer and either a microscope or a scanning laser positioned to receive, by means of said microscope or said scanning laser, images of said successive faces on the block, record said images, and store said recorded images in said computer.
Inventors
- 👤 Russell L. Kerschmann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 19:30
CPC Classifications
G; G01; G01N; G01N1/00; G01N1/02; G01N1/04; G01N1/06
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
130 words
An imagre recording method comprising infiltrating an object with and embedding said object in material containing a UV absorber which maximally absorbs at a wavelength near the maximum absorbance wavelength of a first fluorescent dye, staining said object with said first fluorescent dye, said dye being characterized by a maximum of absorbance wavelength and a minimum absorbance wavelength, embedding said object in a block capable of being cut with a knife, cutting a plurality of slices from said block using said knife to form successfully deeper images of said faces on said block, receiving, by means of a microscope or scanning laser, successively deeper images of the face of said block, recording said successively deeper images of said faces on said block, and storing said recorded images in a computer.
Claim 2
Dependent Claim
130 words
An imagre recording method comprising infiltrating an object with and embedding said object in material containing a UV absorber which maximally absorbs at a wavelength near the maximum absorbance wavelength of a first fluorescent dye, staining said object with said first fluorescent dye, said dye being characterized by a maximum of absorbance wavelength and a minimum absorbance wavelength, embedding said object in a block capable of being cut with a knife, cutting a plurality of slices from said block using said knife to form successfully deeper images of said faces on said block, receiving, by means of a microscope or scanning laser, successively deeper images of the face of said block, recording said successively deeper images of said faces on said block, and storing said recorded images in a computer.
Claim 3
Dependent Claim
127 words
An image recording method comprising staining an object with a first fluorescent dye characterized by a maximum absorbance wavelength and a minimum absorbance wavelength, infiltrating said object with and embedding said object in material containing a second fluorescent dye characterized by a maximum absorbance wavelength near the maximum emssion wavelength of said first fluorescent dye, embedding said object in a block capable of being cut with a knife, cutting a plurality of slices from said block using said knife to form successively deeper images of said faces on said block, receiving, by means of a microscope or scanning laser, successively deeper images of the face of said block, recording said successively deeper images of said faces on said block, and storing said recorded images in a computer.
Claim 4
Dependent Claim
127 words
An image recording method comprising staining an object with a first fluorescent dye characterized by a maximum absorbance wavelength and a minimum absorbance wavelength, infiltrating said object with and embedding said object in material containing a second fluorescent dye characterized by a maximum absorbance wavelength near the maximum emssion wavelength of said first fluorescent dye, embedding said object in a block capable of being cut with a knife, cutting a plurality of slices from said block using said knife to form successively deeper images of said faces on said block, receiving, by means of a microscope or scanning laser, successively deeper images of the face of said block, recording said successively deeper images of said faces on said block, and storing said recorded images in a computer.
Claim 5
Dependent Claim
112 words
An image recording method comprising staining an object with a first fluorescent dye characterized by a maximum absorbance wavelength and a minimum absorbance wavelength, infiltrating said object with and embedding said object in matrial containng a quencher fo said fluorescent dye, embedding said object in a block capable of being cut with a knife, cutting a plurality of slices from said block using said knife to form successively deeper images of said faces on said block, receiving, by means of a microscope or scanning laser, successively deeper images of the face of block, recording said successively deeper images of said faces on said block, and storing said recorded images in a computer.
Claim 6
Dependent Claim
112 words
An image recording method comprising staining an object with a first fluorescent dye characterized by a maximum absorbance wavelength and a minimum absorbance wavelength, infiltrating said object with and embedding said object in matrial containng a quencher fo said fluorescent dye, embedding said object in a block capable of being cut with a knife, cutting a plurality of slices from said block using said knife to form successively deeper images of said faces on said block, receiving, by means of a microscope or scanning laser, successively deeper images of the face of block, recording said successively deeper images of said faces on said block, and storing said recorded images in a computer.
Claim 7
Dependent Claim
120 words
An image recoroding method comprising staining an object with a first fluorescent dye characterized by a maximum absorbance wavelength and a minimum absorbance wavelength, infiltrating said object with and embedding said object in material containing a dye capable of blocking the transmission of visible light through said block, embedding said object in a block capable of beibng cut with a knife, cutting a plurality of slices from said block using said knife to form successively deeper images of said faces on said block, receiving, by means of a microscope or scanning laser, successively deeper images of the faces of said block, recording said successively deeper images of said faces on said block, and storing said recorded images in a computer.
Claim 8
Dependent Claim
120 words
An image recoroding method comprising staining an object with a first fluorescent dye characterized by a maximum absorbance wavelength and a minimum absorbance wavelength, infiltrating said object with and embedding said object in material containing a dye capable of blocking the transmission of visible light through said block, embedding said object in a block capable of beibng cut with a knife, cutting a plurality of slices from said block using said knife to form successively deeper images of said faces on said block, receiving, by means of a microscope or scanning laser, successively deeper images of the faces of said block, recording said successively deeper images of said faces on said block, and storing said recorded images in a computer.
Claim 9
Dependent Claim
103 words
An image recording method comprising staining a tissue sample comprising cells containing DNA with a first fluorescent dye charactrerzied by a maximum absorbance wavelength and a minimum absorbance wavelength, embedding said object in a block capable of being cut with knife, cutting a plurality of slices from said block using said knife to form successively deeper images of said faces on said block, receiving, by means of a microscope or scanning laser, successively deeper images of successively deeper images of said faces on said block, and storing said recorded images in a computer and determining the ploidy of said cells using said computer.
Claim 10
Dependent Claim
103 words
An image recording method comprising staining a tissue sample comprising cells containing DNA with a first fluorescent dye charactrerzied by a maximum absorbance wavelength and a minimum absorbance wavelength, embedding said object in a block capable of being cut with knife, cutting a plurality of slices from said block using said knife to form successively deeper images of said faces on said block, receiving, by means of a microscope or scanning laser, successively deeper images of successively deeper images of said faces on said block, and storing said recorded images in a computer and determining the ploidy of said cells using said computer.
Claim 11
Independent Claim
23 words
The method of any of claims 1, 2, 3, 4 or 5 wherein said visual recording is carried out using a video camera.
Claim 12
Independent Claim
23 words
The method of any of claims 1, 2, 3, 4 or 5 wherein said object is a piece of human or animal tissue.
References Cited (Backward Citations)
US3884563A
Cited
US3973827A
Cited
GB2074749A
Cited
US4377958A
Cited
US4412961A
Cited
US4558438A
Cited
EP0139424A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-10-01 | 1994-04-02 | 1994-10-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-10-01 | 1998-04-02 | 1998-10-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-10-01 | 2002-04-02 | 2002-10-02 | $7700.00 | Window Closed / Lapsed |