US4959355A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of inhibiting osmotic water flow
Assignee: Columbia University
•
Filing: 1987-03-16
•
Granted: 1990-09-25
•
Expires: 2007-03-16
Abstract
Abstract This invention provides a method of inhibiting osmotic water flow across mammalian cell membranes which involves contacting the water channels of the cell membranes with an effective amount of a glucose transport blocker or of digitonin so as to inhibit osmotic water flow across the membranes. Additionally, this invention provides a method of treating a subject afflicted with a condition associated with abnormal osmotic water flow across cell membranes which comprises administering to the subject an effective amount of a glucose transport blocker or of digitonin so as to contact the water channels of the subject's cell membranes and inhibit osmotic water flow across the membranes.
Inventors
- 👤 Jorge Fischbarg
- 👤 Larry S. Liebovitch
- 👤 Jan P. Koniarek
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 18:22
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/70; A61K31/045; A61K31/05; A61K31/12; A61K31/28; A61K31/305; A61K31/33; A61K31/395; A61K31/40
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
36 words
A method of inhibiting osmotic water flow across mammalian cell membranes which comprises contacting the water channels of the cell membranes with a glucose transport blocker so as to inhibit osmotic water flow across the membranes.
Claim 2
Independent Claim
36 words
A method of inhibiting osmotic water flow across mammalian cell membranes which comprises contacting the water channels of the cell membranes with a glucose transport blocker so as to inhibit osmotic water flow across the membranes.
Claim 3
Dependent Claim
24 words
A method of claim 1, wherein the glucose transport blocker is selected from the group, consisting of phloridzin, phloretin, diallyldiethylstilbestrol, cytochalasin B, and ethylidene-D-glucose.
Claim 4
Dependent Claim
20 words
A method of claim 1, wherein the contacting of the water channels of the cell membranes is effected in vitro.
Claim 5
Dependent Claim
24 words
A method of claim 3, wherein the glucose transport blocker is selected from the group consisting of phloridzin, phloretin, diallyldiethylstibestrol, cytochalasin B, and ethylidene-D-glucose.
Claim 6
Independent Claim
33 words
A method of inhibiting osmotic water flow across mammalian cell membranes which comprises contacting the water channels of the cell membranes with digitonin so as to inhibit osmotic water flow across the membranes.
Claim 7
Independent Claim
33 words
A method of inhibiting osmotic water flow across mammalian cell membranes which comprises contacting the water channels of the cell membranes with digitonin so as to inhibit osmotic water flow across the membranes.
Claim 8
Dependent Claim
20 words
A method of claim 5, wherein the contacting of the water channels of the cell membranes is effected in vitro.
Claim 9
Independent Claim
33 words
A method of inhibiting osmotic water flow across mammalian cell membranes which comprises contacting the water channels of the cell membranes with escin so as to inhibit osmotic water flow across the membranes.
Claim 10
Independent Claim
33 words
A method of inhibiting osmotic water flow across mammalian cell membranes which comprises contacting the water channels of the cell membranes with escin so as to inhibit osmotic water flow across the membranes.
Claim 11
Independent Claim
86 words
A method of inhibiting osmotic water flow across corneal endothelium which comprises mounting the tissue in a chamber so as to form two compartments within the chamber, filling the compartments with solutions of different osmolarity, applying a hydrostatic pressure difference across the corneal endothelium to develop a spontaneous fluid transport towards one compartment, including a large osmotic flow by making one said compartment hypertonic, and blocking said osmotic water flow by applying a glucose transport blocker or digitonin to the water channels of the corneal endothelium.
Claim 12
Independent Claim
86 words
A method of inhibiting osmotic water flow across corneal endothelium which comprises mounting the tissue in a chamber so as to form two compartments within the chamber, filling the compartments with solutions of different osmolarity, applying a hydrostatic pressure difference across the corneal endothelium to develop a spontaneous fluid transport towards one compartment, including a large osmotic flow by making one said compartment hypertonic, and blocking said osmotic water flow by applying a glucose transport blocker or digitonin to the water channels of the corneal endothelium.
Claim 13
Dependent Claim
24 words
A method of claim 8, wherein the glucose transport blocker is selected from the group consisting of phloridzin, phloretin, diallyldiethylstibestrol, cytochalasin B, and ethylidene-D-glucose.
References Cited (Backward Citations)
US3151108A
Cited
US3163636A
Cited
US4101652A
Cited
US4470975A
Cited
JPS5788122A
Cited
US4684627A
Cited
US4760135A
Cited
US4665058A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-09-24 | 1994-03-26 | 1994-09-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-09-24 | 1998-03-26 | 1998-09-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-09-24 | 2002-03-26 | 2002-09-25 | $7700.00 | Window Closed / Lapsed |