US4956281A
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DNA sequences, recombinant DNA molecules and processes for producing lymphocyte function associated antigen-3
Assignee: Dana Farber Cancer Institute Inc
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Filing: 1987-06-03
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Granted: 1990-09-11
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Expires: 2007-06-03
Abstract
Abstract Polypeptides that bind to CD2, the receptor on the surface of T-lymphocytes. Most preferably, the polypeptides bind to CD2 on the surface of T-lymphocytes and inhibit adhesion between T-lymphocytes and target cells. DNA sequences that code on expression in appropriate unicellular hosts for those polypeptides. Methods of making and using those polypeptides in therapy and diagnosis.
Inventors
- 👤 Barbara P. Wallner
- 👤 Timothy A. Springer
- 👤 Catherine Hession
- 👤 Richard Tizard
- 👤 Robert Mattaliano
- 👤 Michael L. Dustin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 22:06
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C07K14/70503; C07K14/70528; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56966; G01N33/56972; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
98 words
A DNA sequence selected from the group consisting of: (a) the DNA sequence which codes for the LFA-3 polypeptide carried in phage λHT16; (b) DNA sequences which hybridize under conditions equivalent to about 20° to 27° C. below Tm and 1 M sodium chloride to the LFA-3 DNA sequence of (a) and which code on expression for a polypeptide that binds to CD2, the receptor on the surface of T-lymphocytes; and (c) DNA sequences which code on expression for a polypeptide that binds to CD2 which is coded for on expression by any of the foregoing DNA sequences.
Claim 2
Dependent Claim
98 words
A DNA sequence selected from the group consisting of: (a) the DNA sequence which codes for the LFA-3 polypeptide carried in phage λHT16; (b) DNA sequences which hybridize under conditions equivalent to about 20° to 27° C. below Tm and 1 M sodium chloride to the LFA-3 DNA sequence of (a) and which code on expression for a polypeptide that binds to CD2, the receptor on the surface of T-lymphocytes; and (c) DNA sequences which code on expression for a polypeptide that binds to CD2 which is coded for on expression by any of the foregoing DNA sequences.
Claim 3
Dependent Claim
35 words
The DNA sequence according to claim 1, wherein said DNA sequence (b) codes on expression for a polypeptide that binds to CD2 on the surface of T-lymphocytes and inhibits adhesion between T-lymphocytes and target cells.
Claim 4
Dependent Claim
12 words
The DNA sequence according to claim 2, wherein said polypeptide is soluble.
Claim 5
Dependent Claim
102 words
The DNA sequence according to claim 1, wherein said DNA sequence is selected from the group consisting of a DNA sequence of the formula N 1-1047 of FIG. 3, a DNA sequence of the formula N 17-766 of FIG. 3, a DNA sequence of the formula N 101-766 of FIG. 3, a DNA sequence of the formula N 101-611 -N 716-766 of FIG. 3, a DNA sequence of the formula ATG-N 101-766 of FIG. 3, a DNA sequence of the formula ATG-N 101-661 -N 716-766 of FIG. 3, and DNA sequences that code on expression for any of the above DNA sequences.
Claim 6
Dependent Claim
54 words
The DNA sequence according to claim 4, wherein said DNA sequence is selected from the group consisting of a DNA sequence of the formula N 17-766 of FIG. 3, a DNA sequence of the formula ATG-N 17-766 of FIG. 3, and DNA sequences that code on expression for any of the above DNA sequences.
Claim 7
Dependent Claim
38 words
A recombinant DNA molecule comprising a DNA sequence selected from the group consisting of the DNA sequences of claims 1 to 5, said DNA sequence being operatively linked to an expression control sequence in said recombinant DNA molecule.
Claim 8
Dependent Claim
79 words
The recombinant DNA molecule according to claim 6, wherein said expression control sequence is selected from the group consisting of the early or late promoters of SV40 or adenovirus, the lac system, the trp system, the TAC system, the TRC system, the major operator and promoter regions of phage λ, the control regions of fd coat protein, the promoter for 3-phosphoglycerate kinase or other glycolytic enzymes, the promoters of acid phosphatase and the promoters of the yeast α-mating factors.
Claim 9
Dependent Claim
22 words
A unicellular host transformed with a recombinant DNA molecule selected from the group consisting of the recombinant DNA molecules of claim 6.
Claim 10
Dependent Claim
33 words
The host according to claim 8, wherein said host is selected from the group consisting of strains of E.coli, Pseudomonas, Bacillus, Streptomyces, fungi, animal cells, plant cells and human cells in tissue culture.
Claim 11
Dependent Claim
33 words
A method of producing a LFA-3 polypeptide comprising culturing a unicellular host transformed with a recombinant DNA molecule selected from the group consisting of the recombinant DNA molecules of claim 6 or 7.
References Cited (Backward Citations)
US4728927A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-09-10 | 1994-03-12 | 1994-09-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-09-10 | 1998-03-12 | 1998-09-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-09-10 | 2002-03-12 | 2002-09-11 | $7700.00 | Window Closed / Lapsed |