Multiple level filter device and kit containing same

Claim 1

In a kit for extracting and testing an antigen from body fluids, the kit including an extraction tube to which a body fluid and an extracting reagent are added to produce a solution of extracted antigen, a filter tip for insertion into said tube after adding body fluids and the extracting agent, said tip being constructed to mate with said tube; a test device having plural test wells, filter means in each well for passing the solution through and for attaching the extracted antigen, said filter means having a predetermined average pore size, and an absorbent material underneath said filter means to draw liquid through said filter means and into said absorbent material, said test device being physically separate from said extraction tube; and a container of labeled antibody for said antigen; the improvement wherein said filter tip includes at least two filter media having two different predetermined pore sizes, one being a fine size and the other being a coarse size, said filter medium with said fine size being substantially the same pore size as said pore size of said test device filter means.