US4940666A
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Process and defined medium for growth of human epidermal keratinocyte cells
Assignee: University Patents Inc
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Filing: 1986-12-09
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Granted: 1990-07-10
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Expires: 2006-12-09
Abstract
Abstract Disclosed are novel methods and materials for generating in vitro cultured populations of human epidermal keratinocyte cells having a characteristic colony-forming efficiency of greater than 20%. Novel media preparations and procedures are disclosed that permit isolation, serum-free primary culture and serum-free serial subculture of human epidermal keratinocytes. Also disclosed are procedures and products employing keratinocyte cells grown in serum-free conditions for medical application. i.e., skin grafting.
Inventors
- 👤 Stephen T. Boyce
- 👤 Richard G. Ham
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 03:28
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0625; C12N5/0629; C12N2500/00; C12N2500/70; C12N2500/80; C12N2500/84; C12N2500/90; C12N2501/00; C12N2501/10; C12N2501/11; C12N2501/30; C12N2501/33; C12N2501/38; C12N2501/39; C12N2501/392
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
35 words
A population of human epidermal cells growing in a transferrin-free medium comprising: complete MCDB153; epidermal growth factor at a concentration of 1.0 ng/ml to 25.0 ng/ml; and insulin at a concentration of 0.5 μg/ml to
Claim 2
Dependent Claim
35 words
A population of human epidermal cells growing in a transferrin-free medium comprising: complete MCDB153; epidermal growth factor at a concentration of 1.0 ng/ml to 25.0 ng/ml; and insulin at a concentration of 0.5 μg/ml to
Claim 3
Dependent Claim
79 words
A population of cells as in claim 1 wherein said medium additionally comprises at least one supplement selected from the group consisting of: hydrocortisone at a concentration of 0.14 μM to 28.0 μM; ethanolamine at a concentration of 0.01 mM to 1.0 mM; phosphoethanolamine at a concentration of 0.01 mM to 1.0 mM; calcium chloride at a concentration of 0.03 mM to 1.0 mM; and whole bovine pituitary extract at a protein concentration of 7.0 μg/ml to 700.0 μg/ml.
Claim 4
Dependent Claim
23 words
A population of human epidermal cells as in claim 1 wherein said cells have formed a monolayer or multiple strata in said medium.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-07-09 | 1994-01-08 | 1994-07-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-07-09 | 1998-01-08 | 1998-07-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-07-09 | 2002-01-08 | 2002-07-10 | $7700.00 | Window Closed / Lapsed |