US4933288A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Use of a modified soluble Pseudomonas exotoxin A in immunoconjugates
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1986-11-21
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Granted: 1990-06-12
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Expires: 2006-11-21
Abstract
Abstract DNA sequences encoding proteins processable by secretion leaders in recombinant hosts are described. The DNA sequences encode the NH 2 -terminal region of proteins that are cleaved from a secretion leader and may be secreted through the cell membrane and, if present, cell wall in some cases. Proteins encoded by the DNA sequence have an NH 2 -terminal amino acid sequence conforming to a consensus amino acid sequence that is processable by the particular secretion leader. DNA sequences encoding a consensus amino acid sequence processable by the diphterhia toxin secretion leader are disclosed. A novel Pseudomonas exotoxin and CSF-1 having NH 2 -terminal sequences conforming to a consensus sequence are exemplified.
Inventors
- 👤 I. Lawrence Greenfield
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 11:06
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/21; C07K14/34; C07K14/435; C07K14/52; C07K14/53; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/71; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/01; C07K2319/02
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
65 words
DNA comprising a DNA sequence encoding a mature protein heterologous to a DT leader sequence with an NH 2 terminal consensus sequence comprising about 7 amino acids having substantial homology to the first 7 amino terminal amino acids of mature DT wherein said mature protein is Pseudomonas exotoxin A having a glycine NH 2 -terminal in addition to the coding sequence of Pseudomonas exotoxin A.
Claim 2
Dependent Claim
65 words
DNA comprising a DNA sequence encoding a mature protein heterologous to a DT leader sequence with an NH 2 terminal consensus sequence comprising about 7 amino acids having substantial homology to the first 7 amino terminal amino acids of mature DT wherein said mature protein is Pseudomonas exotoxin A having a glycine NH 2 -terminal in addition to the coding sequence of Pseudomonas exotoxin A.
Claim 3
Dependent Claim
18 words
The DNA sequence of claim 1 wherein said Pseudomonas exotoxin A has the NH 2 -terminal sequence gly-ala-glu-glu-ala-phe-asp.
Claim 4
Dependent Claim
26 words
The DNA sequence of claim 8 wherein said mature protein is CSF-1 having gly-ala NH 2 -terminal in addition to the coding sequence of mature CSF-1.
Claim 5
Dependent Claim
19 words
The DNA sequence of claim 3 wherein said mature protein is CSF-1 having the NH 2 -terminal sequence gly-ala-glu-glu-val.
Claim 6
Dependent Claim
22 words
An expression vector comprising a DNA sequence encoding the DT leader sequence in reading frame with the DNA sequence of claim 1.
Claim 7
Dependent Claim
22 words
An expression vector comprising a DNA sequence encoding the DT leader sequence in reading frame with the DNA sequence of claim 3.
Claim 8
Dependent Claim
22 words
The expression vector of claim 5 wherein the DNA sequence encoding the NH 2 -terminal acid of the DT leader is ATG.
Claim 9
Dependent Claim
24 words
The expression vector of claim 6 wherein the DNA sequence encoding the NH 2 -terminal amino acid sequence of the DT leader is ATG.
Claim 10
Dependent Claim
11 words
A host organism transformed with the expression vector of claim 5.
Claim 11
Dependent Claim
11 words
A host organism transformed with the expression vector of claim 6.
Claim 12
Dependent Claim
11 words
A host organism transformed with the expression vector of claim 7.
Claim 13
Dependent Claim
11 words
A host organism transformed with the expression vector of claim 8.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-06-11 | 1993-12-11 | 1994-06-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-06-11 | 1997-12-11 | 1998-06-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-06-11 | 2001-12-11 | 2002-06-12 | $7700.00 | Window Closed / Lapsed |