US4933280A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Recombinant DNA sequence encoding Alveolar Surfactant Protein
Assignee: Takeda GmbH
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Filing: 1986-04-30
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Granted: 1990-06-12
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Expires: 2006-04-30
Abstract
Abstract The complete coding sequences and amino acid sequences for both canine and human 32K alveolar surfactant proteins (ASP) are disclosed; clones for the 10K protein have also been obtained. Methods and vectors for obtaining these proteins in recombinant form are described. The availability of large amounts of these proteins through recombinant techniques permits the use of ASP in suitable pharmaceutical compositions in the treatment of respiratory deficiency syndromes.
Inventors
- 👤 James W. Schilling, Jr.
- 👤 Robert T. White
- 👤 Barbara Cordell
- 👤 Bradley J. Benson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:04
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; A; A61; A61P; A61P11/00; C07; C07K; C07K1/00; C07K1/14; C07K1/16; C07K14/00; C07K14/435; C07K14/785; C12P; C12P21/00; C12P21/02; A61K; A61K38/00
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
80 words
A recombinant DNA sequence or a naturally occurring mutant DNA sequence thereof, which DNA sequence consists essentially of a DNA sequence which encodes mammalian 10K alveolar surfactant protein (10K ASP), which 10K ASP is encoded by a DNA which hybridizes under stringency conditions corresponding to a wash of 2×SSC, 0.1% SDS at 50° C. to human SP18 cDNA as show for nucleotides 611-1156 in FIG. 6, said DNA being free from DNA encoding additional protein normally produced in lung cells.
Claim 2
Dependent Claim
80 words
A recombinant DNA sequence or a naturally occurring mutant DNA sequence thereof, which DNA sequence consists essentially of a DNA sequence which encodes mammalian 10K alveolar surfactant protein (10K ASP), which 10K ASP is encoded by a DNA which hybridizes under stringency conditions corresponding to a wash of 2×SSC, 0.1% SDS at 50° C. to human SP18 cDNA as show for nucleotides 611-1156 in FIG. 6, said DNA being free from DNA encoding additional protein normally produced in lung cells.
Claim 3
Dependent Claim
26 words
The recombinant DNA of claim 1 wherein the 10K ASP is encoded by the DNA of FIG. 2 or a naturally occurring mutant DNA sequence thereof.
Claim 4
Dependent Claim
26 words
The recombinant DNA of claim 1 wherein the 10K ASP is encoded by the DNA of FIG. 6 or a naturally occurring mutant DNA sequence thereof.
Claim 5
Dependent Claim
34 words
A recombinant expression system comprising the 10K ASP encoding DNA sequence of claim 1 which expression system is capable, when transformed into host cells, of effecting the expression of the DNA of claim 1.
Claim 6
Dependent Claim
12 words
The expression system of claim 4 which further comprises a viral enhancer.
Claim 7
Dependent Claim
14 words
The expression system of claim 5 wherein the viral enhancer is the SV40 enhancer.
Claim 8
Dependent Claim
11 words
Recombinant host cells transformed with the DNA sequence of claim 1.
Claim 9
Dependent Claim
11 words
Recombinant host cells transformed with the expression system of claim 4.
Claim 10
Dependent Claim
9 words
The cells of claim 8 which are mammalian cells.
Claim 11
Dependent Claim
9 words
The cells of claim 9 which are CHO cells.
Claim 12
Dependent Claim
32 words
A method to produce 10K ASP which comprises culturing the cells of claim 7 under conditions which effect the expression of the 10K ASP encoding DNA, and recovering the 10K ASP produced.
Claim 13
Dependent Claim
32 words
A method to produce 10K ASP which comprises culturing the cells of claim 8 under conditions which effect the expression of the 10K ASP encoding DNA, and recovering the 10K ASP produced.
Claim 14
Dependent Claim
25 words
A recombinant expression system of claim 4 wherein the recombinant DNA encoding 10K ASP is operably linked to control sequences compatible with mammalian host cells.
Claim 15
Dependent Claim
15 words
The expression system of claim 13 wherein said control sequences comprise the human metallothionein-2 promoter.
References Cited (Backward Citations)
US4312860A
Cited
US4562003A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-06-11 | 1993-12-11 | 1994-06-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-06-11 | 1997-12-11 | 1998-06-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-06-11 | 2001-12-11 | 2002-06-12 | $7700.00 | Window Closed / Lapsed |