US4917998A
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Method of detecting AIDS virus infection
Assignee: Epitope Diagnostics Inc
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Filing: 1986-05-06
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Granted: 1990-04-17
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Expires: 2006-05-06
Abstract
Abstract A method is disclosed for detecting the presence of HTLV III infected cells in a medium. The method comprises contacting the medium with monoclonal antibodies against an antigen produced as a result of the infection and detecting the binding of the antibodies to the antigen. The antigen may be a gene product of the HTLV III virus or may be bound to such gene product. On the other hand the antigen may not be a viral gene product by may be produced as a result of the infection and may further be bound to a lymphocyte. The medium may be a human body fluid or a culture medium. A particular embodiment of the present method involves a method for determining the presence of an AIDS virus in a person. The method comprises combining a sample of a body fluid from the person with a monoclonal antibody that binds to an antigen produced as a result of the infection and detecting the binding of the monoclonal antibody to the antigen. The presence of the binding indicates the presence of an AIDS virus infection. Also disclosed are novel monoclonal antibodies, novel compositions of matter, and novel diagnostic kits.
Inventors
- 👤 Denis R. Burger
- 👤 Andrew S. Goldstein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 13:49
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/08; C07K16/10; C07K16/112; C07K16/114; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; G01N33/56988; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/974; Y10S435/975
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
115 words
A method for detecting the presence of HTLV III infection, which comprises contacting a sample obtained from a mammalian subject suspected of being infected with HTLV III virus with monoclonal antibodies against an antigen produced as a result of said infection and detecting the binding of said antibodies to said antigen, said antigen being selected from the group consisting of a 28 kilodalton antigen, a 39 kilodalton antigen, a 49 kilodalton antigen, and a 52 kilodalton antigen, the binding thereof being related to the presence of HTLV III infection, said antigen being capable of binding to one of the antibodies secreted by a cell line having ATCC accession numbers HB9100, HB9101, HB9102, HB9114, or HB9115.
Claim 2
Independent Claim
115 words
A method for detecting the presence of HTLV III infection, which comprises contacting a sample obtained from a mammalian subject suspected of being infected with HTLV III virus with monoclonal antibodies against an antigen produced as a result of said infection and detecting the binding of said antibodies to said antigen, said antigen being selected from the group consisting of a 28 kilodalton antigen, a 39 kilodalton antigen, a 49 kilodalton antigen, and a 52 kilodalton antigen, the binding thereof being related to the presence of HTLV III infection, said antigen being capable of binding to one of the antibodies secreted by a cell line having ATCC accession numbers HB9100, HB9101, HB9102, HB9114, or HB9115.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein said sample is a human body fluid.
Claim 4
Dependent Claim
20 words
The method of claim 1 wherein said antigen is a viral gene product or bound to a viral gene product.
Claim 5
Dependent Claim
23 words
The method of claim 1 wherein said antigen is produced as a result of said infection and is not a viral gene product.
Claim 6
Dependent Claim
12 words
The method of claim 1 wherein said monoclonal antibodies are murine antibodies.
Claim 7
Dependent Claim
22 words
The method of claim 1 wherein said monoclonal antibodies are prepared by immunization with a lysate of HTLV-III infected H9 cell culture.
Claim 8
Dependent Claim
13 words
The method of claim 1 wherein said antibody is conjugated to a label.
Claim 9
Dependent Claim
28 words
The method of claim 2 wherein said body fluid is selected from the group consisting of whole blood, lymphatic fluid, serum, plasma, semen, saliva, and cerebral spinal fluid.
Claim 10
Dependent Claim
127 words
An immunoassay method for the detection of an AIDS virus related infection in a human host, which comprises: combining a body fluid from a human host with a monoclonal antibody that binds to an antigen that is present only when said infection is present, said antigen being selected from the group consisting of a 28 kilodalton antigen, a 39 kilodalton antigen, a 49 kilodalton antigen, and a 52 kilodalton antigen, and present, and examining the combination for the presence of immune complexes comprising said antigen and said monoclonal antibody, the presence thereof indicating the presence of an AIDS virus infection, said antigen being capable of binding to one of the antibodies secreted by a cell line having the ATCC accession numbers HB9100, HB9101, HB9102, HB9114, or HB9115.
Claim 11
Dependent Claim
127 words
An immunoassay method for the detection of an AIDS virus related infection in a human host, which comprises: combining a body fluid from a human host with a monoclonal antibody that binds to an antigen that is present only when said infection is present, said antigen being selected from the group consisting of a 28 kilodalton antigen, a 39 kilodalton antigen, a 49 kilodalton antigen, and a 52 kilodalton antigen, and present, and examining the combination for the presence of immune complexes comprising said antigen and said monoclonal antibody, the presence thereof indicating the presence of an AIDS virus infection, said antigen being capable of binding to one of the antibodies secreted by a cell line having the ATCC accession numbers HB9100, HB9101, HB9102, HB9114, or HB9115.
Claim 12
Dependent Claim
21 words
The method of claim 9 wherein said antigen is a viral gene product or is bound to a viral gene product.
Claim 13
Dependent Claim
20 words
The method of claim 9 wherein said antigen is not a viral gene product and is bound to a lymphocyte.
Claim 14
Dependent Claim
11 words
The method of claim 9 wherein said lymphocytes are T-helper cells.
Claim 15
Dependent Claim
14 words
The method of claim 9 wherein said monoclonal antibody binds to a soluble antigen.
Claim 16
Dependent Claim
14 words
The method of claim 9 wherein said monoclonal antibody is conjugated to a label.
Claim 17
Dependent Claim
28 words
The method of claim 9 wherein said body fluid is selected from the group consisting of whole blood, lymphatic fluid, serum, plasma, semen, saliva, and cerebral spinal fluid.
Claim 18
Dependent Claim
101 words
A diagnostic kit comprising in a packaged combination in separate containers a monoclonal antibody capable of binding to an antigen produced as a result of an infection by the viral causative agent of AIDS or a labeled derivative of said antibody, said antigen being selected from the group consisting of a 28 kilodalton antigen, a 39 kilodalton antigen, a 49 kilodalton antigen, and a 52 kilodalton antigen, and a member of a signal producing system, said antigen being capable of binding to one of the antibodies secreted by a cell line having ATCC accession numbers HB9100, HB9101, HB9102, HB9114, or HB9115.
Claim 19
Dependent Claim
101 words
A diagnostic kit comprising in a packaged combination in separate containers a monoclonal antibody capable of binding to an antigen produced as a result of an infection by the viral causative agent of AIDS or a labeled derivative of said antibody, said antigen being selected from the group consisting of a 28 kilodalton antigen, a 39 kilodalton antigen, a 49 kilodalton antigen, and a 52 kilodalton antigen, and a member of a signal producing system, said antigen being capable of binding to one of the antibodies secreted by a cell line having ATCC accession numbers HB9100, HB9101, HB9102, HB9114, or HB9115.
Claim 20
Dependent Claim
27 words
The kit of claim 16 wherein said label is selected from the group consisting of enzymes, radioisotopes, particles, supports, chromogens, fluorescers, chemiluminescers, coenzymes, free radicals, and bacteriophages.
Claim 21
Dependent Claim
26 words
The kit of claim 16 wherein said antibody is unlabeled and said kit further comprises a labeled derivative of a specific binding partner of said antibody.
References Cited (Backward Citations)
EP0136798A2
Cited
US4629783A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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