US4914210A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Oligonucleotide functionalizing reagents
Assignee: Bayer Corp
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Filing: 1987-10-02
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Granted: 1990-04-03
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Expires: 2007-10-02
Abstract
Abstract Oligonucleotide functionalizing reagents are disclosed which are useful in introducing sulfhydryl, amino and additional hydroxyl groups into oligonucleotides. The reagents are substantially linear in structure, at one end provided with a phosphoramidite moiety, at an opposing end provided with a sulfhydryl, amino or hydroxyl moiety, the two ends linked through a hydrophilic spacer chain. Methods of using and synthesizing the novel reagents are disclosed as well.
Inventors
- 👤 Corey Levenson
- 👤 Chu-An Chang
- 👤 Fred T. Oakes
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 22:33
CPC Classifications
C; C07; C07D; C07D209/00; C07D209/02; C07D209/44; C07D209/48; C07C; C07C323/00; C07C323/10; C07C323/11; C07C323/12; C07C43/00; C07C43/02; C07C43/03; C07C43/04; C07C43/10; C07C43/11; C07C43/14; C07C43/178; C07D317/00; C07D317/08; C07D317/10; C07D317/14; C07D317/18; C07D317/22; C07F; C07F9/00; C07F9/02; C07F9/06; C07F9/22; C07F9/24; C07F9/2404; C07F9/2408; C07F9/2433; C07F9/2441; C07F9/2454; C07F9/2458; C07F9/2479; C07F9/2487; C07F9/26; C07F9/547; C07F9/553; C07F9/5537; C07H; C07H21/00; C08; C08G; C08G65/00; C08G65/02; C08G65/32; C08G65/329; C08G65/335; C08G65/3356; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
50 words
An oligonucleotide functionalizing reagent having the structure: ##STR34## wherein R is a protected amino, sulfhydryl, or hydroxyl moiety; R 1 is a protected hydroxymethyl or hydrogen; R 2 is methyl or β-cyanoethyl, R 3 is lower alkyl; and N is an integer in the range of 2 through 20 inclusive.
Claim 2
Dependent Claim
50 words
An oligonucleotide functionalizing reagent having the structure: ##STR34## wherein R is a protected amino, sulfhydryl, or hydroxyl moiety; R 1 is a protected hydroxymethyl or hydrogen; R 2 is methyl or β-cyanoethyl, R 3 is lower alkyl; and N is an integer in the range of 2 through 20 inclusive.
Claim 3
Dependent Claim
12 words
The compound of claim 1, wherein R is a protected amino moiety.
Claim 4
Dependent Claim
12 words
The compound of claim 1, wherein R is a protected sulfhydryl moiety.
Claim 5
Dependent Claim
12 words
The compound of claim 1, wherein R is a protected hydroxyl moiety.
Claim 6
Dependent Claim
8 words
The compound of claim 2 that is ##STR35##
Claim 7
Dependent Claim
8 words
The compound of claim 3 that is ##STR36##
Claim 8
Dependent Claim
8 words
The compound of claim 4 that is ##STR37##
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-04-02 | 1993-10-02 | 1994-04-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-04-02 | 1997-10-02 | 1998-04-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-04-02 | 2001-10-02 | 2002-04-03 | $7700.00 | Window Closed / Lapsed |