US4912038A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Recombinant DNA sequence encoding alveolar surfactant protein
Assignee: Takeda GmbH
•
Filing: 1985-12-13
•
Granted: 1990-03-27
•
Expires: 2005-12-13
Abstract
Abstract The complete coding sequences and amino acid sequences for both canine and human 32K alveolar surfactant proteins (ASP) are disclosed; clones for the 10K protein have also been obtained. Methods and vectors for obtaining these proteins in recombinant form are described. The availability of large amounts of these proteins through recombinant techniques permits the use of ASP in suitable pharmaceutical compositions in the treatment of respiratory deficiency syndromes.
Inventors
- 👤 James W. Schilling, Jr.
- 👤 Robert T. White
- 👤 Barbara Cordell
- 👤 Bradley J. Benson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:38
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/785; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12P; C12P21/00; C12P21/02; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
64 words
An isolated recombinant vector containing a DNA sequence or a naturally occurring mutant DNA sequence thereof, which encodes mammalian 32K alveolar surfactant protein (32K ASP), wherein said 32K ASP encoded by a DNA which hybridizes under conditions represented by a wash stringency of 0.45M NaCl, 0.045M sodium citrrate, 0.1% SDS at 50° C. to the DNA encoding canine 32K ASP shown in FIG. 1.
Claim 2
Dependent Claim
64 words
An isolated recombinant vector containing a DNA sequence or a naturally occurring mutant DNA sequence thereof, which encodes mammalian 32K alveolar surfactant protein (32K ASP), wherein said 32K ASP encoded by a DNA which hybridizes under conditions represented by a wash stringency of 0.45M NaCl, 0.045M sodium citrrate, 0.1% SDS at 50° C. to the DNA encoding canine 32K ASP shown in FIG. 1.
Claim 3
Dependent Claim
31 words
The vector of claim 1 wherein the DNA sequence consists essentially of a DNA sequence which encodes ASP selected from the group consisting of human 32K ASP and canine 32K ASP.
Claim 4
Dependent Claim
29 words
An expression system for mammalian 32K ASP which comprises the recombinant vector of claim 1 wherein the DNA is operably linked to control sequences functional in mammalian host cells.
Claim 5
Dependent Claim
16 words
The expression system of claim 3 wherein said control sequences comprise the human metallothionein II promoter.
Claim 6
Dependent Claim
34 words
The recombinant vector of claim 2 wherein the encoded ASP has the mature amino acid sequence shown in FIG. 1, or the amino acid sequence encoded by a naturally occurring mutant DNA sequence thereof.
Claim 7
Dependent Claim
34 words
The recombinant vector of claim 2 wherein the encoded ASP has the mature amino acid sequence shown in FIG. 3 or the amino acid sequence encoded by a naturally occurring mutant DNA sequence thereof.
Claim 8
Dependent Claim
40 words
An isolated recombinant vector containing a expression system comprising the DNA sequence encoding mammalian ASP of claim 1, wherein said expression system is capable, when transformed into host cells, of effecting the expression of the ASP-encoding DNA of claim 1.
Claim 9
Dependent Claim
12 words
The expression system of claim 3 which further comprises a viral enhancer.
Claim 10
Dependent Claim
14 words
The expression system of claim 8 wherein the viral enhancer is the SV40 enhancer.
Claim 11
Dependent Claim
11 words
Recombinant host cells transformed with the recombinant vector of claim 1.
Claim 12
Dependent Claim
11 words
Recombinant host cells transformed with the expression system of claim 7.
Claim 13
Dependent Claim
9 words
The cells of claim 11 which are mammalian cells.
Claim 14
Dependent Claim
9 words
The cells of claim 12 which are CHO cells.
Claim 15
Dependent Claim
45 words
A method to produce mammalian 32K ASP, which comprises culturing the cells of claim 10 in a culture medium and under conditions wherein the DNA sequence encoding 32K ASP is expressed and secreted in the medium, and recovering the 32K ASP produced from the medium.
Claim 16
Dependent Claim
45 words
A method to produce mammalian 32K ASP, which comprises culturing the cells of claim 11 in a culture medium and under conditions wherein the DNA sequence encoding 32K ASP is expressed and secreted in the medium, and recovering the 32K ASP produced from the medium.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-03-26 | 1993-09-25 | 1994-03-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-03-26 | 1997-09-25 | 1998-03-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-03-26 | 2001-09-25 | 2002-03-27 | $7700.00 | Window Closed / Lapsed |