Novel liposome composition for sustained release of steroidal drugs in lungs

Claim 2

A liposome composition delivered by inhalation consisting essentially of a nonphosphlipid lipid mixture of cholesterol and cholesterol salt, said salt being selected from the group consisting of sulphatae, phosphate, nitrate and maleate, the ratio of cholesterol salt to cholesterol to a drug being from 30 to 70 mole % of cholesterol salt, from 20 to 50 mole % of of a cholesterol and 0.01 to 20 mole % of the steroidal drug.

Claim 3

The composition of claim 1, wherein the cholesterol salt is cholesterol sulfate.

Claim 4

The composition of claim 1, wherein the ratio is 50:40:10.

Claim 5

The composition of claim 1, wherein the ratio is 55:40:5.

Claim 6

The composition of claim 3, wherein the steroidal drug is selected from the group consisting of aldosterone, beclomethasone, betamethasone, cholesterol, cloprednol, cortisone, cortivazol, deoxycortone, desonide, dexamethasone, difluorocortolone, fluclorolone, fluorocortisone, flumethasone, flunisolide, fluocinolone, fluocinonide, fluorocortolone, fluorometholone, flurandrenolone, halcinonide, hydrocortisone, meprednisone, methylprednisolone, paramethasone, prednisolone, prednisone and triamicinolone, or their respective pharmaceutically acceptable salts or esters.

Claim 7

The composition of claim 5 wherein the steroidal drug is beclomethasone dipropionate.

Claim 8

The composition of claim 5, wherein the steroidal drug is betamethasone.

Claim 9

The composition of claim 5, wherein the steroidal drug is dexamethasone.

Claim 12

The method of claim 9, wherein the composition comprises 30 to 70 mole % of cholesterol sulfate, 20 to 50 mole % of cholesterol and from 0.01 to 20 mole % of a steroidal drug.

Claim 13

The method of claim 10, wherein the drug is selected from the group consisting of aldosterone, beclomethasone, or its dipropionate, betamethasone, cholesterol, cloprednol, cortisone, cortivazol, deoxycortone, desonide, dexamethasone, difluorocortolone, fluclorolone, fluorocortisone, flumethasone, flunisolide, fluocinolone, fluocinonide, fluorocortolone, fluorometholone, flurandrenolone, halcinonide, hydrocortisone, meprednisone, methylprednisolone, paramethasone, prednisolone, prednisone and triamicinolone, or their respective pharmaceutically acceptable salts or esters.

Claim 14

The method of claim 11, wherein the composition is 50 mole % of cholesterol sulfate, 40 mole % of cholesterol and 10 mole % of beclomethasone dipropionate.

Claim 15

The method of claim 11, wherein the composition is 50 mole % of cholesterol sulfate, 40 mole % of cholesterol and 10 mole % of dexamethasone.

Claim 16

An inhalation method for treatment of lung diseases by administering through the inhalation route to the person in need of such treatment a therapeutically effective amount of liposome composition consisting essentially of a steroidal drug and a nonphospholipid lipid mixture of cholesterol and cholesterol salt, said salt being selected from the group consisting of sulphate, phosphate, nitrate and meleate.

Claim 17

An inhalation method for treatment of lung diseases by administering through the inhalation route to the person in need of such treatment a therapeutically effective amount of liposome composition consisting essentially of a steroidal drug and a nonphospholipid lipid mixture of cholesterol and cholesterol salt, said salt being selected from the group consisting of sulphate, phosphate, nitrate and meleate.

Claim 18

The method of claim 14, wherein the composition comprises 30 to 70 mole % of cholesterol sulfate, 20 to 50 mole % of cholesterol and 0.01 to 20 mole % of a steroidal drug.

Claim 19

The method of claim 15, wherein the drug is selected from the group consisting of aldosterone, beclomethasone, or its dipropionate, betamethasone, cholesterol, cloprednol, cortisone, cortivazol, deoxycortone, desonide, dexamethasone, difluorocortolone, fluclorolone, fluorocortisone, flumethasone, flunisolide, fluocinolone, fluocinonide, fluorocortolone, fluorometholone, flurandrenolone, halcinonide, hydrocortisone, meprednisone, methylprednisolone, paramethasone, prednisolone, prednisone and triamicinolone, or their respective pharmaceutically acceptable salts esters.

Claim 20

The method of claim 16, wherein the composition is 50 mole % of cholesterol sulfate, 40 mole % of cholesterol and 10 mole % of beclomethasone dipropionate.

Claim 21

The method of claim 16, wherein the composition is 50 mole % of cholesterol sulfate, 40 mole % of cholesterol and 10 mole % of dexamethasone.

Claim 22

A process of preparing a liposome steroidal composition comprising: (a) mixing a cholesterol salt, cholesterol and a steroid drug in an appropriate ratio; (b) dissolving the mixture in a solvent; (c) lyophilizing the mixture; (d) resuspending the residue in a buffer; (e) preparing the liposomes; and (f) optionally extruding the steroidal liposomes through the filter.

Claim 23

A process of preparing a liposome steroidal composition comprising: (a) mixing a cholesterol salt, cholesterol and a steroid drug in an appropriate ratio; (b) dissolving the mixture in a solvent; (c) lyophilizing the mixture; (d) resuspending the residue in a buffer; (e) preparing the liposomes; and (f) optionally extruding the steroidal liposomes through the filter.

Claim 24

The process of claim 19, wherein a cholesterol salt is cholesterol sulfate.

Claim 25

The process of claim 20, wherein the ratio of cholesterol sulfate to cholesterol to steroidal drug is from 30 to 70 mole % of cholesterol sulfate; from 20 to 50 mole % of cholesterol and from 0.01 to 20 mole % of the steroidal drug.

Claim 26

The process of claim 21, wherein the ratio of cholesterol sulfate to cholesterol to the drug is 50:40:10 mole % and drug is beclomethasone dipropionate.

Claim 27

The process of claim 22, wherein the solvent is chosen from the group consisting of alcohol, chloroform, dimethylsulfoxide, dimethylformamide or the mixture thereof.

Claim 28

The process of claim 23, wherein the solvent is dimethylsulfoxide.